LOCUS       sequence-49524-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  TITLE     NHEJ
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDR121
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..108)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(109..120)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttttgaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
       61 tctagtactt ctcgacaagc ttggtaccta gtgatggtga tggtgatggc gtccctcgat
      121 ctcgaggtcc tggaagtgct tggtgagggc ttccagcagc tcctgcttct tcagcccact
      181 cttcagcccg taagcccggc aggcctcttt cagcatgggc acagtgaact tgcccagcgt
      241 acccttgctg atgtgggtct tcagctcctc ttctgaatac tccaccttgg gccttttgct
      301 tccagaacct tcattatcgt gttttctctt ggtaactttc ccttcaggat tgtaatctgg
      361 tgggtaaaca agctccttaa actcatccac caaggagccc agtcttttat tcattgcttc
      421 aaccttgggc aatgtcaggt ccactgcttg ttccggctcc atcaaatcca aggccaaggc
      481 ctccaggttc ctgaagtgct gctgcagcac ggggttctca aagctgtcac ttctgtatgt
      541 gaagcgaagc ttctcaacga tagccttcat cttgcccacc tgctctggag ttgccatgat
      601 tttttcagta aagggcatct tccttttatc atcagcaaag ggtaaaaaga ccagctggaa
      661 gcctggagga gtcacctga
//
