LOCUS       sequence-495273-       17057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct for C-terminal S 
            protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 
            D1315-146.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17057)
  AUTHORS   Zhang H, Xiong Y, Sun Y, Park JM, Su D, Feng X, Keast S, Tang M, 
            Huang M, Wang C, Srivastava M, Yang C, Zhu D, Chen Z, Li S, Yin L, 
            Pommier Y, Chen J
  TITLE     RAD54L2-mediated DNA damage avoidance pathway specifically preserves
            genome integrity in response to topoisomerase 2 poisons.
  JOURNAL   Sci Adv. 2023 Dec 8;9(49):eadi6681. doi: 10.1126/sciadv.adi6681. 
            Epub 2023 Dec 6.
  PUBMED    38055811
REFERENCE   2  (bases 1 to 17057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-12-8"; volume: "9(49):eadi6681. doi"; pages: " 
            10.1126/sciadv.adi6681. Epub 2023 Dec 6"
COMMENT     Sequence Label: pLEX307-RAD54L2-D1315-1467-SFB
FEATURES             Location/Qualifiers
     source          1..17057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(116..220)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(246..701)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(383..404)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     595..614
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     828..850
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     842..859
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     843..859
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     869..888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        869..887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      908..1043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1083..1204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1094..1113)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1148..1167
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             1282..1515
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1587..2175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1693..1704
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2102..2122
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2217..2330)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(2340..2363)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2367..2390)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(2424..2468)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    2506..2530
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6485..6509)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6529..7707)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6538..7476
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6561..6581)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7845..7962
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8005..8607)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     promoter        complement(8617..8946)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8631..8766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8931..8951
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     9013..9030
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    9213..9237
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10471..10495)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(10568..10681)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             complement(10691..10714)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(10718..10741)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(10775..10819)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        complement(10879..11082)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10908..10928)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11083..11386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(11464..11880)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(11480..11496)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(11512..11534)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    12081..12280
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             12343..12936
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(13035..13634)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(13666..13862)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(13674..13884)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13680..13815
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(14115..14159)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14344..14577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    15070..15195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             15242..15422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(15423..15649)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(15677..15697)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(15677..15695)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(15716..15732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15716..15732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15729..15751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15740..15756
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15764..15770,15771..15788,15789..15794))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15809..15830
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15947..15964)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16118..16706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16198..16217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
       61 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
      121 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
      181 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
      241 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
      301 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      361 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      421 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      481 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      541 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      601 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      661 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      721 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      781 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      841 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
      901 gtccctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg
      961 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     1021 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggatagctag agccagacat
     1081 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     1141 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     1201 agttcctctc actctctgat attcatttct ttgcaagtta taaatactga ataataagat
     1261 gacatgaact actatacgta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     1321 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1381 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1441 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt
     1501 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     1561 tgtaagtcat tggtcttaaa gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt
     1621 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
     1681 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
     1741 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga
     1801 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     1861 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
     1921 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
     1981 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     2041 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     2101 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     2161 atccagaggt tgattgtcga cttaacgcgt tgattgtcga cttaaccggt actttagggc
     2221 tcccgctggc cctgagggtg gtgctccagc ctggcccgca gctgctccag ctcgccggcc
     2281 aggccctcca ccacgtggcc gccccgccag ccggtggtct tctcgtccat cgacgcgcct
     2341 ttgtcatcgt catccttgta atctcccttg tcgtcatcgt ctttgtagtc aggcgcgtca
     2401 gcgccggctc ctgcgccggc tccgctgtcc atgtgctggc gttcgaattt agcagcagcg
     2461 gtttctttac cagcactacc agcactacca gcactatcca tatcaaccac tttgtacaag
     2521 aaagctgggt ccaactcccc agcctgggag gtgaaggggg tggtgaggtt atggtttaag
     2581 gagacgggtg gcatggcaaa cccgccagag actggatacc cgtgttcata cggctgcacg
     2641 ggggctggca gatgaccctt cctggaccgc cggcgggatg actcctgggc agcaggtggt
     2701 gtggccaact ttcgcttgtg gccccttaag tccagcactg ggtgcctgtc accacagggc
     2761 tggccagtca ccaagaggga actattgagg caatgacccc ctagtcgagg ctcagttccg
     2821 agagctgtcc cgggaacagc actgctgtcc ataagttggg ccggggggcc aggcagggcg
     2881 gcattggtgc ttggggagct ctgacgggat tcccgggcag cagccacatc tgcatactgc
     2941 tgaagctcac tgatgatctc tgggctgtca ggagagacgg gcctggccag cccctcaggg
     3001 tcgggggctt tgggtgatga ggcactgtgt ctgccgttgc ttgtggactc gcaagaaggt
     3061 ccctgggaac ctgaggcagc catccgacca tcttcaggaa cctttggttt gccagttgcc
     3121 cggacagcaa agatccgtcc atcactggta cggatgtacg tcccttttgt cccacggatg
     3181 atgtggatgc tctcaccagc attaattctt gcctgtacat ctgtggagct gttgagtcca
     3241 ggaataacaa tatctgtcgt ggtgaccacc ttctgcacaa ggactcctgc acgctgcaag
     3301 tagttgatgg gaaagttcat ggatggattt gtgctggagg cagagcttac acttcctccc
     3361 aatgggacat gccggggcat catggggatg ggggtggact gcacaggacg aacactggcc
     3421 acaggctttt catcaccctt caaagttggc tgccagtttc tctggctgaa ggcggggatg
     3481 ctggtcaggc tctgatcgct ggcagggtaa tactgcgcat acgatgggcg ggtatagggg
     3541 actgatgtgc gtttgtcttc ctcatagctt ttctttgctg cttttttctc agccttggtt
     3601 agcttgtgat cctttcggtt caagagcaat gactcatgct cgaaaggctc cttggtgatg
     3661 aggtgagggt acttcagaca ggccagttgc aggactgact ccttgatccc ctttacgttc
     3721 aaggaaactt ggggagctgg ctccttctca acaaagtgca gtaggttttc cacctctttc
     3781 cgtgtgaagt tcagcattgg atttagatca tccaccaccc gatctgacat gccctgcttg
     3841 gaaatctgac ggtcatagat cttcttttct agagtgtaat cagccacaag gcgatagatg
     3901 taacagggct ttttctggcc ataacggtat acccgacata ctgcctgggc atcatggcaa
     3961 gggttccagg aagcatcaaa caccaccact cggttggcac caatcagatt cacacccaag
     4021 catccggccc ttgtagagag aaggaacagc caggtggtga ggttgctggg atcattgaac
     4081 tgattaataa gccgctccct ctcaaaggca ggggtgctac catctagccg gaagtagctg
     4141 atgtttcgaa cccacttctg tgctccttgc ccctcggtac caggtggaca gggtacttct
     4201 cgttttccaa ggaattcctc gatgagagcc aaggtggaaa gactctggct aaacacaagg
     4261 atcttgtccc caagcttcac actttcctca atcaggtgga aaagcagtac catcttggga
     4321 gagttttcta ggactccagt ctggtaatta gtcagaaggt ccttggccca ttcatatgtg
     4381 acaatgttgt tgcctcgctc ctggaaaggg ttgaagccaa cgccctgtag gaacttgcta
     4441 ttggttgcct ctcccatcga ggaagccaag gtgctatcct ctcccttgcc ttttgttccc
     4501 tgtggtggac agcgggcact ggtccctgca gagccaagtt cttccacgtc taggtcctgc
     4561 tcattggcca agctctcctt ctgaagggct tcatacagca catcagggtg attccagatc
     4621 ttgcaacaca cacagaatgc cttaaggggg ttcagcccca gccaaccgct gctaccacag
     4681 tcccggaagc gatccatgaa ctgtgtatac aaatctcgct ggatcttgga gagccgcaca
     4741 aggatcacat tttcttcctt ggcagggaga tgaatcttca gcacagtgtg gcctctcctc
     4801 tgcacaaagc cctccagaag actgtgcagg acatggctcc ggtaccgcat gaggcggacg
     4861 tcctgaggtg tgctgtcaat acattgccca ttcaggatag ggcgttcaaa catgttgctg
     4921 aactcctgcc gggtgccaag gaagtctggg cgcacaaagt ccaccatgca ccagtactca
     4981 atgaggttgt tttgcagagg gtacccagtc agcaccaccc ggcggcgaga gcggatattc
     5041 ttcagagcct gtgaggtgct ggcctggcag tttttgatgc ggtgtccctc atcacagatt
     5101 actacatcag ggccagggcg gcataaagcc ttctcaaact ctctccgaaa ctcctgctgc
     5161 cgatcttcct catctagatc aatgatgact gggtgagaac gcttcttggt tttcttcggt
     5221 ctacctgtgg caaatgattt cttcagagtg aggagtctgt acatctcgta ccccatcagc
     5281 agcacgccac cctctgacac ccaatcagcc atcactttag cacgagatgc catcgtcttg
     5341 tgctcatcat tcaagatgtg aactttaaag aaccgaggct ggacttcttc aggcttgttg
     5401 tcagccggga gggcttcagg aggtggaagc cacatgttga actctgccag ccaattctga
     5461 agagtattaa ccggcacaat ggcaaggact gttttggctg gcgtgtggcg gaagaggacg
     5521 tcgatgaaag agatcacttg caaagttttc cccagaccca tgctgtgggc cagaatacag
     5581 ccaaagccac tgctggtctt aaacctctcc agagactcca ctaggttatc gtaaaggaac
     5641 cggatcccgc caatctgatg aggtttcaca gcccgtgcca actgtggggc aaggaagaca
     5701 ttttcctcct ctggagggtg gtttaggttg acaaggaccc gcccaagggc gtcacgctgg
     5761 tttaagacat cattgacatg ggtgccaccc ttctcttctt cctcatcatc ttcactgaca
     5821 gattcactgc tgtccacaat gtgcagagtg tcctcctctc cagagctcag ttcaatcacc
     5881 tcatctcgac tgcttttttc atcctcactg ccactgctac tgtccaaaca aatgacttcc
     5941 tgggccaaga cccgaggagg cagttgggga ccgtcacttg cccttaaagc aatttcctca
     6001 gggaggaact ccagcggaac agtaggaata ggggctgcat aatctttcct ctgctgctcc
     6061 aggcgcttcc ttctttccaa ctcttcttgc tgtgctgctt tggtaacagg ctccaattga
     6121 tcctcccgga gtagctttcg tatgtttctt ctcatgtggg agggcttctg agctctcttc
     6181 tttttggggt cctcggaggc taggttcttg ccttggtggc gcctaagctg ctctgaaggc
     6241 tcagactgag atgaggtagt tgaagtgcac cgcggcggct gctgcccatc ttctcccaac
     6301 tgggcatgct cagtgccaca catgccttcc agggatgggt catccagcag gtcttcttca
     6361 tcatccctgt cacactcctc cactgccacc tcctcctcct cctcctcctc ttccgcatcc
     6421 tccagctcca cgtccgggtc caggtctgga tcgctccctg aggcagattc gtctgacatg
     6481 gtgaagcctg cttttttgta caaacttgtt gatcaatcga tgctagcctc acgacacctg
     6541 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
     6601 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
     6661 ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga
     6721 cgacgtactc caaaagctcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
     6781 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
     6841 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg
     6901 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg
     6961 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta
     7021 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg
     7081 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc
     7141 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa
     7201 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat
     7261 tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag gcgaaggggc
     7321 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag
     7381 aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat aacccgtaaa
     7441 gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg caaacccgtt
     7501 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7561 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7621 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7681 ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct agaaggtcca
     7741 ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcacct gcaggcctcg
     7801 agccggcgcc aaagtggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     7861 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     7921 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     7981 atttgtctca agatctagaa ttcgttaact ggcaccgggc ttgcgggtca tgcaccaggt
     8041 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
     8101 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
     8161 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
     8221 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
     8281 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
     8341 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
     8401 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
     8461 cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg tggcggggta
     8521 gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc gggtggcgag
     8581 gcgcaccgtg ggcttgtact cggtcatggt aagctttttg caaaagccta ggcctccaaa
     8641 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     8701 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     8761 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     8821 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     8881 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     8941 acacacattc cacagggtcg accactgtgc tggcgaattc ctacgcttcc ttcacgacat
     9001 tcaacagacc ttgcattcct ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga
     9061 agacagggcc aggtttccgg gccctcacat tgccaaaaga cggcaatatg gtggaaaata
     9121 acatatagac aaacgcacac cggccttatt ccaagcggct tcggccagta acgttagggg
     9181 ggggggaggg agaggggcgg atccctgctc gagccacttt gtacaagaaa gctgggtttt
     9241 attaatggcc tctttcgtat ttctttcttt tcttggttgg gctgttctga attgtagata
     9301 atctttcagt gtttgtggct aagccggttt gctgtgcctg ccctgggtta tttttcataa
     9361 gatgtctgga ggagagactt tccaaaacat gcaactcttc cttagacgtg gtttttgcca
     9421 aatcttgttt caaaagtaac cctggatctg acaactctga agctggcaaa accctgacag
     9481 ctttgctttt ttccactatt atttctctcg ggactaatcg atcacttcca agagaaactc
     9541 tcccgtggtg gtcgaagtct tccgcagagc tgcagttcct gcgtttctgt tgggttttgg
     9601 ccactcgtgg actttgtttt ggacatggtg atgctgactc acagctacag acggggctcc
     9661 tgcttagaag ccctgtcttt aaccgctccc caggatgagg tttttgctgg ccactagtgt
     9721 cttctggctg ggtctgatta actttttcca gcttttgaac aggaagcccc cagtggctct
     9781 cagctgcctt tatggaatcc tttgcttgtc ccatggattc tgatggggga ctggaggaag
     9841 cctgaccatc attcctcctc cttgctatct ctgcaatcac tgagtcacta gatgtttcca
     9901 cagtgtctct tctggtctgc gatttgctcg tgacactgct ttttgtttca gttcttttca
     9961 caatttcttt cagagcattt ctccggagct ttgcactggg aggaagtgaa ctctctgcac
    10021 actcagcaaa gtaatcagac actccatgga gaactctttc tgtcaaatct tcattctgac
    10081 ttgctaaaag atcacgactg aatgcaagct gactgacaca aaccacaaat ctgtcaggga
    10141 atacacagag ttcagcattc tgtgtgctcc tgatgcagcg aaggcgtatc cctctcttga
    10201 ggaaataggc tgtgatttga aagccataac cttttaagga caagggattg gatcttgtga
    10261 attctgcatt tgatggcctg tgctgtttgc gaacttccaa gtactcctga actcttgaat
    10321 caatggtttc ttttaacagt aaacagactt cctctttctt ctgttcagaa aaccagcttg
    10381 tatctttgtt tgaaccttgt ggcaagatat gaagatccac gaggacagca aaattcccac
    10441 atttaacagc aaatttctta gatgccatga agcctgcttt tttgtacaaa cttgtctctc
    10501 gagcgatggg cccgcggtac cgtcgactgc agaattcagg cgcgccgccc agcttgcagc
    10561 cgccggaggg ctcccgctgg ccctgagggt ggtgctccag cctggcccgc agctgctcca
    10621 gctcgccggc caggccctcc accacgtggc cgccccgcca gccggtggtc ttctcgtcca
    10681 tcgacgcgcc tttgtcatcg tcatccttgt aatctccctt gtcgtcatcg tctttgtagt
    10741 caggcgcgtc agcgccggct cctgcgccgg ctccgctgtc catgtgctgg cgttcgaatt
    10801 tagcagcagc ggtttctttc atggtggcct cgagatctga gtccggtagc gctagcggat
    10861 ctgacggttc actaaaccag ctctgcttat atagacctcc caccgtacac gcctaccgcc
    10921 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
    10981 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
    11041 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
    11101 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
    11161 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
    11221 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
    11281 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
    11341 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
    11401 tgggctatga actaatgacc ccgtaattga ttactattag taccaccaca ctgggatccg
    11461 gccggcgcct agagaaggag tgagggctgg ataaagggag gatcgaggcg gggtcgaacg
    11521 aggaggttca agggggagag acggggcgga tggaggaaga ggaggcggag gcttagggtg
    11581 tacaaagggc ttgacccagg gaggggggtc aaaagccaag gcttcccagg tcacgatgta
    11641 ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag accttgatct taacctgggt
    11701 gatgaggtct cggttaaagg tgccgtctcg cggccatccg acgttaaagg ttggccattc
    11761 tgcagagcag aaggtaaccc aacgtctctt cttgacatct accgactggt tgtgagcgat
    11821 ccgctcgaca tctttccagt gacctaaggt caaacttaag ggagtggtaa cagtctggcc
    11881 ctaattttca gacaaataca gaaacacagt cagacagaga caacacagaa cgatgctgca
    11941 gcgctgcagc agacaagacg cgcggcttcg gttccaaacc gaaagcaaaa attcagacgg
    12001 aggcgggaac tgttttaggt tctcgtctcc taccagaacc acatatcctg acggggtcgg
    12061 attccacatc gactcccttc ctcaggtcgg gccacaaaaa cggcccccaa agtccctggg
    12121 acgtctccca gggttgcggc cgggtgttca gaactcgtca gttccaccac gggtccgcca
    12181 gatacagagc tagttagcta actagtaccg acgcaggcgc ataaaatcag tcatagacac
    12241 tagacaatcg gacagacaca gataagttgc tggccagctt gcctcccggt ggtgggtcgg
    12301 tggtccctgg gcaggggtct cccgatcccg gacgagcccc caaatgaaag acccccgctg
    12361 acgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca caagtcggat
    12421 gcaactgcaa gagggtttat tggatacacg ggtacccggg cgactcagtc aatcggagga
    12481 ctggcgcccc gagtgagggg ttgtgggctc ttttattgag ctcggggagc agaagcgcgc
    12541 gaacagaagc gagaagcgaa ctgattggtt agttcaaata aggcacaggg tcatttcagg
    12601 tccttggggc accctggaaa catctgatgg ttctctagaa actgctgagg gctggaccgc
    12661 atctggggac catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc
    12721 ctgtttggcc catattcagc tgttccatct gttcttggcc ctgagccggg gcaggaactg
    12781 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttcc tgaccttgat
    12841 ctgaacttct ctattctcag ttatgtattt ttccatgcct tgcaaaatgg cgttacttaa
    12901 gctagcttgc caaacctaca ggtggggtct ttcattcccc cctttttctg gagactaaat
    12961 aaaatctttt attttatcga tgcatggggt cgtgcgctcc tttcggtcgg gcgctgcggg
    13021 tcgtggggcg ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
    13081 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
    13141 ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg
    13201 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc
    13261 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
    13321 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
    13381 cccgcttcga cgctctccgg cgtggtccag accgccacsg cggcgccgtc gtccgcgacc
    13441 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
    13501 cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
    13561 gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc
    13621 ttgtactcgg tcatggtaag ctccggtggt acctctagaa agctttttgc aaaagcctag
    13681 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
    13741 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
    13801 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagacg
    13861 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
    13921 ctaattgaga tgcaatgcat gggtcccctc ggggttggga ggtgggtctg aaacgataat
    13981 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
    14041 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
    14101 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
    14161 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
    14221 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
    14281 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
    14341 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
    14401 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
    14461 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
    14521 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
    14581 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
    14641 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
    14701 tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct tacttttgtt
    14761 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
    14821 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
    14881 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
    14941 aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
    15001 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
    15061 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
    15121 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
    15181 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
    15241 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
    15301 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
    15361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
    15421 ccgtttatgt atcgagctag gcacttaaat acaatatctc tgcaatgcgg caattcagtg
    15481 gttcgtccaa tccatgtcag acccgtctgt tgccttccta ataaggcacg atcgtaccac
    15541 cttacttcca ccaatcggca tgcacggtgc tttttctctc cttgtaaggc atgttgctaa
    15601 ctcatcgtta ccatgttgca agactacaag agtattgcat aagactacat taagcttgca
    15661 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
    15721 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    15781 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    15841 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    15901 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    15961 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    16021 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    16081 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    16141 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    16201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    16261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    16321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    16381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    16441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    16501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    16561 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    16621 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    16681 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    16741 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    16801 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    16861 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    16921 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    16981 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    17041 atttatcagc aataaac
//
