LOCUS       sequence-495274-       11007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding human FCRL3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, 
            Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G,
            Monticelli S
  TITLE     FCRL3 is an immunoregulatory receptor that restrains the activation 
            of human memory T lymphocytes
  JOURNAL   J Exp Med (2026) 223 (1): e20242474.
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med 
            (2026) 223 (1): e20242474."
COMMENT     Sequence Label: pLVX-EF1_-FCRL3-IRES-puro
FEATURES             Location/Qualifiers
     source          1..11007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    634..749
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(1012..1056)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1241..1474
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1971..2096
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2143..2776
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2944..3078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2968..2987)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3022..3041
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3126..3230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3231..3299,3300..4091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3449..3468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4262..4850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4751..4770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5004..5021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5138..5159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5174..5179,5180..5197,5198..5204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5212..5228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5217..5239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5236..5252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5236..5252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             5380..6013
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6220..6808
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6326..6337
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6735..6755
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6825..7424)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6908..6928)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7405..7424
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    7432..8004
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7820..7837
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(join(10265..11007,1..440))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(10274..11007,1..209)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(10297..10317)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggccccagcg cccaagccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc
       61 aaggcctcga actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact
      121 gcagccaggg gcgtggaagt aattcaaggc acgcaagggc cataacccgt aaagaggcca
      181 ggcccgcggg aaccacacac ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa
      241 agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg
      301 cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg
      361 gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc
      421 cactgacggg caccggagcc tcacgcatgc tcttctccac ctcagtgatg acgagagcgg
      481 gcgggtgagg gggcgggaac gcagcgatct ctgggttcta cgttagtggg agtttaacga
      541 cggtccctgg gattccccaa ggcaggggcg agtccttttg tatgaattac tcatcgataa
      601 actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt aatttgtttt
      661 tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc ccctgcactg
      721 taccccccaa tccccccttt tcttttaaag gcgataccgt cgagatccgt tcactaatcg
      781 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
      841 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
      901 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
      961 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     1021 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     1081 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     1141 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     1201 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     1261 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     1321 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     1381 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     1441 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     1501 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     1561 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     1621 cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg
     1681 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
     1741 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
     1801 cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt
     1861 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     1921 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     1981 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     2041 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     2101 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     2161 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     2221 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     2281 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     2341 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     2401 cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag
     2461 tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac
     2521 tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg
     2581 gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag
     2641 caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca
     2701 gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct tctttgggag
     2761 tgaattagcc cttccaacta ctaagtttgt agtacatatt taacaaatac aatttcttta
     2821 aaatgaaaat aattcagagg aatcacaggt ttagagtaaa tgaaaccaca ggtaattggc
     2881 agtggtaata gggtatgggg tgggaagttt gggatgattt tggttagctt gagttatcca
     2941 gttgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3001 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3061 agctgcaata aacaagttga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa
     3121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     3241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     3301 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     3361 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     3421 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     3481 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     3541 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     3601 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     3661 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3721 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3781 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3841 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3901 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3961 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4021 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4081 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4141 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4201 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4261 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4321 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4381 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     4441 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4501 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4561 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4621 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4681 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4741 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4801 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4861 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4921 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4981 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     5101 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     5161 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     5221 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg
     5281 tcgacggtaa acgctagcaa tgtcaaggcc tctcactctc tgatattcat ttctttgcaa
     5341 gttataaata ctgaataata agatgacatg aactactact gctagagatt ttccacactg
     5401 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5461 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5521 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     5581 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct
     5641 ggaaagtccc cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac
     5701 tccggatgca gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct
     5761 aacacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg
     5821 ttctctcctt tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac
     5881 catccaaagg tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg
     5941 aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg ttgggagtga
     6001 attagccctt ccagtcccct cttttctttt aaaaagtggc taagatctac agctgccttg
     6061 taagtcattg gtcttaaagg tacctgaggt gtgactggaa aacccacctc ctcctcctct
     6121 tgtgcttcta gccaggcaca atcagcattg gtagctgctg tattgctact tgtgattgct
     6181 ccatgttttt ctaggtctcg actgcagaat taattccagg cggggaggcg gcccaaaggg
     6241 agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca
     6301 ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc
     6361 cgcgcagaat ccaggtggca acacaggcga gcagccatgg aaaggacgtc agcttccccg
     6421 acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc
     6481 aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga
     6541 gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca
     6601 ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt
     6661 tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta
     6721 aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt
     6781 cacaaatttt gtaatccaga ggttgattgt tccagagcga tcgctcaggc accgggcttg
     6841 cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag
     6901 ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc
     6961 ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc
     7021 tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc
     7081 ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc
     7141 atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag
     7201 accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc
     7261 gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg gtcgacggtg
     7321 tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc ccgggggacg
     7381 tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatgctaag cttatcatcg
     7441 tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt ttttaacctc
     7501 gactaaacac atgtaaagca tgtgcaccga ggccccagat cagatcccat acaatggggt
     7561 accttctggg catccttcag ccccttgttg aatacgcttg aggtgagcca tttgactctt
     7621 tccacaacta tccaactcac aacgtggcac tggggttgtg ccgcctttgc aggtgtatct
     7681 tatacacgtg gcttttggcc gcagaggcac ctgtcgccag gtggggggtt ccgctgcctg
     7741 caaagggtcg ctacagacgt tgtttgtctt caagaagctt ccagaggaac tgcttccttc
     7801 acgacattca acagaccttg cattcctttg gcgagagggg aaagacccct aggaatgctc
     7861 gtcaagaaga cagggccagg tttccgggcc ctcacattgc caaaagacgg caatatggtg
     7921 gaaaataaca tatagacaaa cgcacaccgg ccttattcca agcggcttcg gccagtaacg
     7981 ttaggggggg gggagggaga ggggcgggat ccgcggccgc ctagtggtct gaggccagta
     8041 atacacgtgg tacattctca tagttttctt catcatcttc ttcatgggcc ctgcctctgc
     8101 tgctagcctc ccctgcagag tcgtctgggt gtgtcttctt cagttctgaa tagaggactg
     8161 taagttcctc atgctcttga tgcatcattg gacaattagc tgagttttct tttgtatgct
     8221 ggatgctcca gatctgggaa taaatcgggt tgctatctcc aggatttaca ttgctgtaca
     8281 ttggctccag ctccattggg gctagtggtt tagagtgagt gggctcttga gggtctatcc
     8341 tggaaggcct ggacgaggaa ggctcctgac actcactagg actgtgacta gatgttccag
     8401 tggcagaaag tcctcctggt ttccttcggg ccctggcgta atgcagcaga gcagcagcag
     8461 cagcaaggac gaggatgctg agcaccagcc ccgtgattcc cgcagcggta aggcctgttc
     8521 tgttcctgga agttcctgta acattgagtg tcaccacttt actgtgctgg gcccccaggc
     8581 cattgtcagc ctcacatgag tagtttccag aatgttctgt agtcagagag aggttgaagg
     8641 atgcccctcc tccagagtgg gccgagatgt tccccaaggt gtcatcctcg tgataaaacc
     8701 agtacaggat cgggaaggag cctctcaggg actcacagtg aagctccagc aggtccccca
     8761 ccacagcctg ggccccggga gccctgaggg tgaggacggg gcgagacacc ggaactgtga
     8821 ccctgagact cactccatga ctgtgctgag ctcccaggcc attgtctgca tcacaggagt
     8881 agtttccaga atgttctgca gtcagagaga ggttgaagga ggctcctcct ccagaggggg
     8941 ctgagctgtt ccccagggtg acatcctcat gataaaatcg gtacaggatc gggggagagc
     9001 ctctcaggga ctcacagtga agctccagca ggtcccccac cacagtgtgg gccctgggag
     9061 ccctgaaggt gaggacaggg tgagataccg gaattctcac ggtgactcga atccacgtgc
     9121 tgaggatggg gctgtgaacg ttatcagctg cacagtagta tctccctgca tcactctcct
     9181 tcacggtgag aacatgcagc tctgccaaca gggaacgctg ggtctttcta cccaggcttc
     9241 ttactcttcc ttctttgtgc caggagaatg tgacagtccc tgaaccctgg gctactgagc
     9301 aaataaggac catattttct ccttcaatca gctgccctcc ggtgggccgg atctctagat
     9361 tcacattaga cacagggact ctctgtacac gtatctgaga tctcaggctc ctttttttga
     9421 tgctgtgagt cactgtctcc acctcacacc agtaagaccc tgagtcttca gtccacatgg
     9481 cagggatctg gagtctgggg gacctgctcc agcccaatcc gagggtctgg ctatctctga
     9541 agagggagaa ttgcagctgg acatctggcc tctgtggaga gagctgggtc tcacaggtca
     9601 gggtcatggg actcccctct atgggcgtgg aagagctggc tctcagcaca ggatgtagaa
     9661 acagctcttg aacttggata tttaggggtt ttgaagttac ttcaatgtca agtatgtaaa
     9721 acttcctata agcagtacaa tgatatttgc tattatccct ggagactgaa ttcactgtga
     9781 tcttctctaa attataacta ttaggaagct gttttccatc cttgtagtaa accttttgat
     9841 gagtgttttt gttgtctttc ccctgacatc tcagaatgac attgtctcct tcaaagacag
     9901 gatgtaaagc ctgcaggatc agccagtcag gtgaaaattc cacatgcacg gcatcactga
     9961 gggaggatcc tcgggtctta cattggtaat ttccaggctc tgtaatttgg atcttgtcat
    10021 gttttatttt caacaacttc tcatcgtgat accaatatgt gtctccctgg gctagggaat
    10081 gtgatatgct gctgcatatg agagccactt tttctccttt gaaggctgtg gaccatggag
    10141 gatcgaggag aagtacagct tttggggcca cccctgattg ttctcttcca ggagtcagga
    10201 tcagcagcag cagccacaga agcatggtgg cactagtctc gaggaattca ccggaaatag
    10261 atcctcacga cacctgaaat ggaagaaaaa aactttgaac cactgtctga ggcttgagaa
    10321 tgaaccaaga tccaaactca aaaagggcaa attccaagga gaattacatc aagtgccaag
    10381 ctggcctaac ttcagtctcc acccactcag tgtggggaaa ctccatcgca taaaacccct
    10441 ccccccaacc taaagacgac gtactccaaa agctcgagaa ctaatcgagg tgcctggacg
    10501 gcgcccggta ctccgtggag tcacatgaag cgacggctga ggacggaaag gcccttttcc
    10561 tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg cgtcctccat tttgagctcc
    10621 ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc aactggtgcc gaccgggcca
    10681 gccttgccgc ccagggcggg gcgatacacg gcggcgcgag gccaggcacc agagcaggcc
    10741 ggccagcttg agactacccc cgtccgattc tcggtggccg cgctcgcagg ccccgcctcg
    10801 ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa
    10861 taccagtgtg cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg
    10921 cagcaggtca tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc
    10981 gaaggtgcca ccagattcgc acgcggc
//
