LOCUS       sequence-495275-       11082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding a chimeric protein composed of the 
            transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa
            595-687; first 93 residues), fused to the extracellular domain of 
            EGFR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, 
            Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G,
            Monticelli S
  TITLE     FCRL3 is an immunoregulatory receptor that restrains the activation 
            of human memory T lymphocytes
  JOURNAL   J Exp Med (2026) 223 (1): e20242474.
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med 
            (2026) 223 (1): e20242474."
COMMENT     Sequence Label: pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro
FEATURES             Location/Qualifiers
     source          1..11082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    163..735
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(330..347)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             743..1342
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(743..762)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1239..1259
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1359..1947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1412..1432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1830..1841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2154..2787
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2915..2931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2915..2931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2928..2950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2939..2955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2963..2969,2970..2987,2988..2993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3008..3029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3146..3163)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3317..3905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3397..3416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4076..4867,4868..4936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4699..4718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4937..5041)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5089..5223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5126..5145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5180..5199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5391..6024
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6071..6196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6693..6926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7111..7155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7418..7533
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7727..8909
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7958..8900
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8857..8877
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cactagcccc aatggagctg gagccaatgt acagcaatgt aaatcctgga gatagcaacc
       61 cgatttattc ccagatctgg agcatccagc atacaaaaga aaactcagct aattgtccaa
      121 tgatgcatca agagcatgag gaataggcgg ccgcggatcc cgcccctctc cctccccccc
      181 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
      241 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
      301 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
      361 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
      421 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
      481 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
      541 tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac aaggggctga aggatgccca
      601 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
      661 ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
      721 aaaacacgat gataagctta gcatgaccga gtacaagccc acggtgcgcc tcgccacccg
      781 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
      841 gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt
      901 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
      961 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     1021 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     1081 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     1141 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     1201 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     1261 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     1321 gacccgcaag cccggtgcct gagcgatcgc tctggaacaa tcaacctctg gattacaaaa
     1381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     1441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     1501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     1561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     1621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     1801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     1861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     1921 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     1981 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     2041 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     2101 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     2161 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     2221 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     2281 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     2341 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     2401 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     2461 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     2521 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     2581 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2641 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2701 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2761 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     2821 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac
     2881 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     2941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     3061 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3481 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3721 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     4081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     4141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     4201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     4261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     4321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     4381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     4441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     4501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     4561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     4621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     4681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     5041 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
     5101 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5161 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5221 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     5281 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     5341 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     5401 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     5461 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     5521 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     5581 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     5641 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     5701 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     5761 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     5821 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5881 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5941 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6001 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6061 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6121 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6181 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6241 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6301 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6361 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6421 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     6481 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     6541 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     6601 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     6661 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     6721 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     6781 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     6841 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     6901 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6961 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7021 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7081 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7141 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     7201 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     7261 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     7321 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     7381 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
     7441 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     7501 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     7561 tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg ggaatcccag
     7621 ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc ccgccccctc
     7681 acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct ccggtgcccg
     7741 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     7801 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     7861 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     7921 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     7981 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacgcccct
     8041 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     8101 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cttgggcgct
     8161 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     8221 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     8281 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     8341 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     8401 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     8461 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     8521 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     8581 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     8641 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     8701 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     8761 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     8821 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     8881 gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc ctcgagacta
     8941 gttctagaat gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc
     9001 tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc
     9061 tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact
     9121 gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct
     9181 tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc
     9241 gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg
     9301 ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga
     9361 gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca
     9421 acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga
     9481 tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga
     9541 gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc
     9601 agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg
     9661 caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag
     9721 ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg
     9781 atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta
     9841 attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga
     9901 tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta
     9961 acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac
    10021 acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg
    10081 gtgactcctt cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg
    10141 taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc
    10201 atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc
    10261 ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg
    10321 atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga
    10381 aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca
    10441 gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc
    10501 cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca
    10561 agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt
    10621 gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca
    10681 actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag
    10741 gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc
    10801 acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa
    10861 cgaatgggcc taagatcccg tccgctgcgg gaatcacggg gctggtgctc agcatcctcg
    10921 tccttgctgc tgctgctgct ctgctgcatt acgccagggc ccgaaggaaa ccaggaggac
    10981 tttctgccac tggaacatct agtcacagtc ctagtgagtg tcaggagcct tcctcgtcca
    11041 ggccttccag gatagaccct caagagccca ctcactctaa ac
//
