LOCUS       sequence-495277-       10953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding a chimeric protein composed of the 
            transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa
            595-644; first 50 residues), fused to the extracellular domain of 
            EGFR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, 
            Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G,
            Monticelli S
  TITLE     FCRL3 is an immunoregulatory receptor that restrains the activation 
            of human memory T lymphocytes
  JOURNAL   J Exp Med (2026) 223 (1): e20242474.
REFERENCE   2  (bases 1 to 10953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med 
            (2026) 223 (1): e20242474."
COMMENT     Sequence Label: pLVX-EF1_-EGFR- FCRL3-50aa-Chimera-puro
FEATURES             Location/Qualifiers
     source          1..10953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    607..722
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(985..1029)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1214..1447
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1944..2069
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2116..2749
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    2917..3051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2941..2960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2995..3014
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3099..3203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3204..3272,3273..4064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3422..3441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4235..4823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4724..4743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4977..4994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5111..5132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5147..5152,5153..5170,5171..5177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5185..5201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5190..5212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5209..5225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5209..5225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             5353..5986
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6193..6781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6299..6310
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6708..6728
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6798..7397)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6881..6901)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7378..7397
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    7405..7977
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     7793..7810
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     promoter        complement(join(10184..10953,1..413))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(10193..10953,1..182)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(10216..10236)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc
       61 caacccgaag ctcgggatca agaatcacgt actgcagcca ggggcgtgga agtaattcaa
      121 ggcacgcaag ggccataacc cgtaaagagg ccaggcccgc gggaaccaca cacggcactt
      181 acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt
      241 atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc
      301 cagtttaccc cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac
      361 ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga gcctcacgca
      421 tgctcttctc cacctcagtg atgacgagag cgggcgggtg agggggcggg aacgcagcga
      481 tctctgggtt ctacgttagt gggagtttaa cgacggtccc tgggattccc caaggcaggg
      541 gcgagtcctt ttgtatgaat tactcatcga taaactggat ctctgctgtc cctgtaataa
      601 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
      661 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
      721 aaggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
      781 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
      841 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
      901 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
      961 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     1021 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     1081 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     1141 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     1201 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     1261 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     1321 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     1381 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     1441 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     1501 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     1561 gtccctcata tctcctcctc caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg
     1621 gtcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc
     1681 tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct
     1741 tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat
     1801 gtttctaaca ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata
     1861 tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat
     1921 ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg
     1981 ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct
     2041 tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc
     2101 ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg agggatctct
     2161 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     2221 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     2281 ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct
     2341 cgccactccc cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc
     2401 ttgtagcaag ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg
     2461 tgatgaaatg ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc
     2521 atcccatgca ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct
     2581 accttatctg gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg
     2641 acccctggcc ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc
     2701 cacagatcaa ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt
     2761 tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca
     2821 ggtttagagt aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag
     2881 tttgggatga ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt
     2941 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     3001 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tgatctcccg
     3061 atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     3181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     3241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     3301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     3361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     3421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     3481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     3541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     3601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     3661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     5161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     5221 atgaccatga ttacgccaag ctctagctag aggtcgacgg taaacgctag caatgtcaag
     5281 gcctctcact ctctgatatt catttctttg caagttataa atactgaata ataagatgac
     5341 atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     5401 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     5461 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     5521 accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc
     5581 cactccccag tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg
     5641 tagcaagctc gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga
     5701 tgaaatgcta ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc
     5761 ccatgcaggc tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc
     5821 ttatctggct caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc
     5881 cctggccctg gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac
     5941 agatcaagga tatcttgtct tcgttgggag tgaattagcc cttccagtcc cctcttttct
     6001 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtacctga
     6061 ggtgtgactg gaaaacccac ctcctcctcc tcttgtgctt ctagccaggc acaatcagca
     6121 ttggtagctg ctgtattgct acttgtgatt gctccatgtt tttctaggtc tcgactgcag
     6181 aattaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     6241 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     6301 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     6361 cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca
     6421 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     6481 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     6541 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     6601 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     6661 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     6721 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     6781 tgttccagag cgatcgctca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct
     6841 tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg
     6901 cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg
     6961 gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg
     7021 gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc
     7081 tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc
     7141 gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg
     7201 acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg
     7261 acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac
     7321 gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg
     7381 ggcttgtact cggtcatgct aagcttatca tcgtgttttt caaaggaaaa ccacgtcccc
     7441 gtggttcggg gggcctagac gttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     7501 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     7561 ttgaatacgc ttgaggtgag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     7621 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     7681 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
     7741 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
     7801 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
     7861 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
     7921 cggccttatt ccaagcggct tcggccagta acgttagggg ggggggaggg agaggggcgg
     7981 gatccgcggc cgcctacatt ggggctagtg gtttagagtg agtgggctct tgagggtcta
     8041 tcctggaagg cctggacgag gaaggctcct gacactcact aggactgtga ctagatgttc
     8101 cagtggcaga aagtcctcct ggtttccttc gggccctggc gtaatgcagc agagcagcag
     8161 cagcagcaag gacgaggatg ctgagcacca gccccgtgat tcccgcagcg gacgggatct
     8221 taggcccatt cgttggacag ccttcaagac ctggcccagt gcatccgtag gtgcagtttg
     8281 gatggcacag gtggcacaca tggccggcgt ctgcgtactt ccagaccagg gtgttgtttt
     8341 ctcccatgac tcctgccggg caggtcttga cgcagtgggg gccgtcaatg tagtgggcac
     8401 actggataca gttgtctggt ccccgtcctg tgcaggtgat gttcatggcc tgaggcaggc
     8461 actctgggtg gcactgtatg cactcagagt tctccacaaa ctcccttggc tcaccctcca
     8521 gaaggttgca cttgtccacg cattccctgc ctcggctgac attccggcaa gagacgcagt
     8581 ccctgggctc cgggccccag cagccctcgg gggagcacaa ggcatggcag acctggcctg
     8641 tggccttgca gctgttttca cctctgttgc ttataatttt ggttttctga ccggaggtcc
     8701 caaacagttt tttccagttt attgtatttg catagcacaa atttttgttt cctgaaatta
     8761 tcacatctcc atcacttatc tccttgaggg agcgtaatcc caaggatgtt atgttcaggc
     8821 tgacgactgc aagagaaaac tgaccatgtt gcttggtcct gccgcgtatg atttctaggt
     8881 tctcaaaggc atggaggtcc gtcctgtttt caggccaagc ctgaatcagc aaaaaccctg
     8941 tgatttcctt tacggttttc agaatatcca gttcctgtgg atccagagga ggagtatgtg
     9001 tgaaggagtc acccctaaat gccaccggca ggatgtggag atcgccactg atggaggtgc
     9061 agtttttgaa gtgtttaata ttcgtagcat ttatggagag tgagtcttta aattcaccaa
     9121 tacctattcc gttacacact ttgcggcaag gcccttcgca cttcttacac ttgcggacgc
     9181 cgtcttcctc catctcatag ctgtcggccc cacaggctcg gacgcacgag ccgtgatctg
     9241 tcaccacata attacgggga cacttcttca cgcaggtggc accaaagctg tatttgccct
     9301 cggggttcac atccatctgg tacgtggtgg ggttgtagag catgagtggg gggcaggtgt
     9361 ccttgcacgt ggcttcgtct cggaatttgc ggcagaccag gcagtcgctc tcccgggggc
     9421 ctgtgcagcc tgcagcacac tggttgtggc agcagtcact gggggacttg ccacggcagc
     9481 gcccggagca ctgctgggca cagatgattt tggtcagttt ctggcagttc tcctctcctg
     9541 caccccagca gctcccattg ggacagcttg gatcacactt ttggcagctg cccaggtggt
     9601 tctggaagtc catcgacatg ttgctgagaa agtcactgct gactatgtcc cgccactgga
     9661 tgctctccac gttgcacagg gcagggttgt tgctgaaccg cacggcgcca tgcaggattt
     9721 cctgtaaatt tctcatgggc agctccttca gtccggtttt atttgcatca tagttagata
     9781 agactgctaa ggcataggaa ttttcgtagt acatatttcc tctgatgatc tgcaggtttt
     9841 ccaaaggaat tcgctccact gtgttgaggg caatgaggac ataaccagcc acctcctgga
     9901 tggtctttaa gaaggaaaga tcataattcc tctgcacata ggtaatttcc aaattcccaa
     9961 ggaccacctc acagttattg aacatcctct ggaggctgag aaaatgatct tcaaaagtgc
    10021 ccaactgcgt gagcttgtta ctcgtgcctt ggcaaacttt cttttcctcc agagcccgac
    10081 tcgccgggca gagcgcagcc agcagcgcca ggagcgctgc cccggccgtc ccggagggtc
    10141 gcattctaga actagtctcg aggaattcac cggaaataga tcctcacgac acctgaaatg
    10201 gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa
    10261 aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca
    10321 cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg
    10381 tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt
    10441 cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg
    10501 cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg
    10561 gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg
    10621 cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc
    10681 gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg
    10741 cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc
    10801 ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt
    10861 aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca
    10921 cgcggcggcc ccagcgccca agccaggcct caa
//
