LOCUS       sequence-495278-       11102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human FCRL3 with 3xFLAG tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Bianchi N, Foli E, Mostanfar M, Marzi R, Spinella MC, Polletti S, 
            Pecoraro M, Cassotta A, Thakur R, Jarrossay D, Sallusto F, Natoli G,
            Monticelli S
  TITLE     FCRL3 is an immunoregulatory receptor that restrains the activation 
            of human memory T lymphocytes
  JOURNAL   J Exp Med (2026) 223 (1): e20242474.
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med 
            (2026) 223 (1): e20242474."
COMMENT     Sequence Label: pLVX-EF1_-FCRL3-3xFLAG-puro
FEATURES             Location/Qualifiers
     source          1..11102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             71..148
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
     misc_feature    173..745
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(340..357)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             753..1352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(753..772)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1249..1269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1369..1957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1422..1442)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1840..1851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2164..2797
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2925..2941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2925..2941)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2938..2960)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2949..2965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2973..2979,2980..2997,2998..3003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3018..3039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3156..3173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3327..3915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3407..3426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4086..4877,4878..4946))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4709..4728
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4947..5051)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5099..5233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5136..5155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5190..5209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5401..6034
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6081..6206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6703..6936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7121..7165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7428..7543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7737..8919
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7968..8910
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8867..8887
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agaagatgat gaagaaaact atgagaatgt accacgtgta ttactggcct cagaccaccg
       61 gccgctcgag gattacaagg atgacgacga taagggagat tacaaggatg acgacgataa
      121 gatcgattac aaggatgacg acgataaggt ttaaacgcgg ccgcggatcc cgcccctctc
      181 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
      241 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      301 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
      361 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
      421 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
      481 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
      541 gttggatagt tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac aaggggctga
      601 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
      661 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      721 tttcctttga aaaacacgat gataagctta gcatgaccga gtacaagccc acggtgcgcc
      781 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
      841 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
      901 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
      961 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     1021 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     1081 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     1141 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     1201 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     1261 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     1321 cctggtgcat gacccgcaag cccggtgcct gagcgatcgc tctggaacaa tcaacctctg
     1381 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     1441 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     1501 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     1561 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     1621 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     1681 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     1741 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     1801 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     1861 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     1921 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag
     1981 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     2041 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     2101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg
     2161 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     2221 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     2281 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     2341 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     2401 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     2461 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     2521 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     2581 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     2641 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     2701 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     2761 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     2821 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc
     2881 tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     2941 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3001 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3061 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3121 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa
     5101 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5161 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5221 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     5281 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     5341 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     5401 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     5461 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     5521 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     5581 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5641 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     5701 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     5761 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     5821 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     5881 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5941 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6001 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     6061 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6121 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     6181 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6241 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6301 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6361 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6421 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     6481 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     6541 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6601 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     6661 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     6721 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     6781 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     6841 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     6901 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     6961 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7021 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7081 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7141 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7201 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7261 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7321 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     7381 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     7441 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     7501 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     7561 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     7621 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     7681 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     7741 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     7801 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     7861 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     7921 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     7981 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     8041 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     8101 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     8161 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     8221 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     8281 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     8341 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     8401 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     8461 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     8521 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     8581 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     8641 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     8701 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     8761 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     8821 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     8881 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     8941 ctcgagacta gtgccaccat gcttctgtgg ctgctgctgc tgatcctgac tcctggaaga
     9001 gaacaatcag gggtggcccc aaaagctgta cttctcctcg atcctccatg gtccacagcc
     9061 ttcaaaggag aaaaagtggc tctcatatgc agcagcatat cacattccct agcccaggga
     9121 gacacatatt ggtatcacga tgagaagttg ttgaaaataa aacatgacaa gatccaaatt
     9181 acagagcctg gaaattacca atgtaagacc cgaggatcct ccctcagtga tgccgtgcat
     9241 gtggaatttt cacctgactg gctgatcctg caggctttac atcctgtctt tgaaggagac
     9301 aatgtcattc tgagatgtca ggggaaagac aacaaaaaca ctcatcaaaa ggtttactac
     9361 aaggatggaa aacagcttcc taatagttat aatttagaga agatcacagt gaattcagtc
     9421 tccagggata atagcaaata tcattgtact gcttatagga agttttacat acttgacatt
     9481 gaagtaactt caaaacccct aaatatccaa gttcaagagc tgtttctaca tcctgtgctg
     9541 agagccagct cttccacgcc catagagggg agtcccatga ccctgacctg tgagacccag
     9601 ctctctccac agaggccaga tgtccagctg caattctccc tcttcagaga tagccagacc
     9661 ctcggattgg gctggagcag gtcccccaga ctccagatcc ctgccatgtg gactgaagac
     9721 tcagggtctt actggtgtga ggtggagaca gtgactcaca gcatcaaaaa aaggagcctg
     9781 agatctcaga tacgtgtaca gagagtccct gtgtctaatg tgaatctaga gatccggccc
     9841 accggagggc agctgattga aggagaaaat atggtcctta tttgctcagt agcccagggt
     9901 tcagggactg tcacattctc ctggcacaaa gaaggaagag taagaagcct gggtagaaag
     9961 acccagcgtt ccctgttggc agagctgcat gttctcaccg tgaaggagag tgatgcaggg
    10021 agatactact gtgcagctga taacgttcac agccccatcc tcagcacgtg gattcgagtc
    10081 accgtgagaa ttccggtatc tcaccctgtc ctcaccttca gggctcccag ggcccacact
    10141 gtggtggggg acctgctgga gcttcactgt gagtccctga gaggctctcc cccgatcctg
    10201 taccgatttt atcatgagga tgtcaccctg gggaacagct cagccccctc tggaggagga
    10261 gcctccttca acctctctct gactgcagaa cattctggaa actactcctg tgatgcagac
    10321 aatggcctgg gagctcagca cagtcatgga gtgagtctca gggtcacagt tccggtgtct
    10381 cgccccgtcc tcaccctcag ggctcccggg gcccaggctg tggtggggga cctgctggag
    10441 cttcactgtg agtccctgag aggctccttc ccgatcctgt actggtttta tcacgaggat
    10501 gacaccttgg ggaacatctc ggcccactct ggaggagggg catccttcaa cctctctctg
    10561 actacagaac attctggaaa ctactcatgt gaggctgaca atggcctggg ggcccagcac
    10621 agtaaagtgg tgacactcaa tgttacagga acttccagga acagaacagg ccttaccgct
    10681 gcgggaatca cggggctggt gctcagcatc ctcgtccttg ctgctgctgc tgctctgctg
    10741 cattacgcca gggcccgaag gaaaccagga ggactttctg ccactggaac atctagtcac
    10801 agtcctagtg agtgtcagga gccttcctcg tccaggcctt ccaggataga ccctcaagag
    10861 cccactcact ctaaaccact agccccaatg gagctggagc caatgtacag caatgtaaat
    10921 cctggagata gcaacccgat ttattcccag atctggagca tccagcatac aaaagaaaac
    10981 tcagctaatt gtccaatgat gcatcaagag catgaggaac ttacagtcct ctattcagaa
    11041 ctgaagaaga cacacccaga cgactctgca ggggaggcta gcagcagagg cagggcccat
    11101 ga
//
