LOCUS       sequence-495282-        7837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7837)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..7837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3007..3016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3013..4443
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    4565..4686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4602..4621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4656..4675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4693..5148)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4830..4851)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5042..5061
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5175..5279
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5281..5638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5281..5472
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5304..5324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5489..5624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5673..6467
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5673..6467
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5727..5746)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6337..6356
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6655..6674)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6699..6746
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7075..7663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7564..7583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagac cgccatgaat ttacaaccaa ttttctggat tggactgatc
      601 agttcagttt gctgtgtgtt tgctcaaaca gatgaaaata gatgtttaaa agcaaatgcc
      661 aaatcatgtg gagaatgtat acaagcaggg ccaaattgtg ggtggtgcac aaattcaaca
      721 tttttacagg aaggaatgcc tacttctgca cgatgtgatg atttagaagc cttaaaaaag
      781 aagggttgcc ctccagatga catagaaaat cccagaggct ccaaagatat aaagaaaaat
      841 aaaaatgtaa ccaaccgtag caaaggaaca gcagagaagc tcaagccaga ggatattact
      901 cagatccaac cacagcagtt ggttttgcga ttaagatcag gggagccaca gacatttaca
      961 ttaaaattca agagagctga agactatccc attgacctct actaccttat ggacctgtct
     1021 tactcaatga aagacgattt ggagaatgta aaaagtcttg gaacagatct gatgaatgaa
     1081 atgaggagga ttacttcgga cttcagaatt ggatttggct catttgtgga aaagactgtg
     1141 atgccttaca ttagcacaac accagctaag ctcaggaacc cttgcacaag tgaacagaac
     1201 tgcaccagcc catttagcta caaaaatgtg ctcagtctta ctaataaagg agaagtattt
     1261 aatgaacttg ttggaaaaca gcgcatatct ggaaatttgg attctccaga aggtggtttc
     1321 gatgccatca tgcaagttgc agtttgtgga tcactgattg gctggaggaa tgttacacgg
     1381 ctgctggtgt tttccacaga tgccgggttt cactttgctg gagatgggaa acttggtggc
     1441 attgttttac caaatgatgg acaatgtcac ctggaaaata atatgtacac aatgagccat
     1501 tattatgatt atccttctat tgctcacctt gtccagaaac tgagtgaaaa taatattcag
     1561 acaatttttg cagttactga agaatttcag cctgtttaca aggagctgaa aaacttgatc
     1621 cctaagtcag cagtaggaac attatctgca aattctagca atgtaattca gttgatcatt
     1681 gatgcataca attccctttc ctcagaagtc attttggaaa acggcaaatt gtcagaagga
     1741 gtaacaataa gttacaaatc ttactgcaag aacggggtga atggaacagg ggaaaatgga
     1801 agaaaatgtt ccaatatttc cattggagat gaggttcaat ttgaaattag cataacttca
     1861 aataagtgtc caaaaaagga ttctgacagc tttaaaatta ggcctctggg ctttacggag
     1921 gaagtagagg ttattcttca gtacatctgt gaatgtgaat gccaaagcga aggcatccct
     1981 gaaagtccca agtgtcatga aggaaatggg acatttgagt gtggcgcgtg caggtgcaat
     2041 gaagggcgtg ttggtagaca ttgtgaatgc agcacagatg aagttaacag tgaagacatg
     2101 gatgcttact gcaggaaaga aaacagttca gaaatctgca gtaacaatgg agagtgcgtc
     2161 tgcggacagt gtgtttgtag gaagagggat aatacaaatg aaatttattc tggcaaattc
     2221 tgcgagtgtg ataatttcaa ctgtgataga tccaatggct taatttgtgg aggaaatggt
     2281 gtttgcaagt gtcgtgtgtg tgagtgcaac cccaactaca ctggcagtgc atgtgactgt
     2341 tctttggata ctagtacttg tgaagccagc aacggacaga tctgcaatgg ccggggcatc
     2401 tgcgagtgtg gtgtctgtaa gtgtacagat ccgaagtttc aagggcaaac gtgtgagatg
     2461 tgtcagacct gccttggtgt ctgtgctgag cataaagaat gtgttcagtg cagagccttc
     2521 aataaaggag aaaagaaaga cacatgcaca caggaatgtt cctattttaa cattaccaag
     2581 gtagaaagtc gggacaaatt accccagccg gtccaacctg atcctgtgtc ccattgtaag
     2641 gagaaggatg ttgacgactg ttggttctat tttacgtatt cagtgaatgg gaacaacgag
     2701 gtcatggttc atgttgtgga gaatccagag tgtcccactg gtccagacat cattccaatt
     2761 gtagctggtg tggttgctgg aattgttctt attggccttg cattactgct gatatggaag
     2821 cttttaatga taattcatga cagaagggag tttgctaaat ttgaaaagga gaaaatgaat
     2881 gccaaatggg acacgggtga aaatcctatt tataagagtg ccgtaacaac tgtggtcaat
     2941 ccgaagtatg agggaaaagg tagcgcatcc gggggtgcgt caggcggcag cggatctgga
     3001 ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     3061 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     3121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     3181 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     3241 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     3301 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     3361 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     3421 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     3481 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     3541 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     3601 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     3661 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     3721 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     3781 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     3841 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     3901 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     3961 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     4021 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     4081 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     4141 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     4201 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     4261 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     4321 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4381 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     4441 taagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     4501 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     4561 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4621 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4681 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     4741 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     4801 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     4861 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     4921 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     4981 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5041 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5101 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     5161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     5281 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5341 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5401 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5461 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5521 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5581 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     5641 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     5701 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5761 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5821 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     5881 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5941 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6001 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6061 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6121 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6181 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6241 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6301 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6361 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6421 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6481 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     6541 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6601 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     6661 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     6721 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     6781 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     6841 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     6901 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     6961 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7021 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7741 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     7801 cggggtcatt agttcatagc ccatatatgg agttccg
//
