LOCUS       sequence-49531-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Ernst P, Wang J, Huang M, Goodman RH, Korsmeyer SJ
  TITLE     MLL and CREB bind cooperatively to the nuclear coactivator 
            CREB-binding protein.
  JOURNAL   Mol Cell Biol. 2001 Apr . 21(7):2249-58.
  PUBMED    11259575
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2001 Apr . 21(7):2249-58."
COMMENT     Sequence Label: 3695 pGEX4T-3 MLL AD mut.E
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(324..346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(491..509)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        577..681
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atccctgaaa tcagattcag acaataacaa cagtgatgac
       61 tgtgggaata tcctgcctgc ggacattatg gactttgtac taaagaatac tccatccatg
      121 caggctttgg gtgagagccc agagtcatct tcatcagaac tcctgaatct tggtgaagga
      181 ttgggtcttg aatccctgca agccgaattc cagcacactg gcggccgtta ctagtggatc
      241 cccgaattcc cgggtcgact cgagcggccg catcgtgact gactgacgat ctgcctcgcg
      301 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      361 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      421 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga
      481 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      541 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      601 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      661 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      721 tttgcggcat tttgccttcc tgtttttgct caccagaacg ctggtgaagt aaaatgtga
//
