LOCUS       sequence-495355-        2523 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2523)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30002
FEATURES             Location/Qualifiers
     source          1..2523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..264
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     201..222
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(364..381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(535..1123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(615..634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1294..2085)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2307..2411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2479..2497
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
       61 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      121 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
      181 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      241 ctcggcatgg acgagctgta caagggtaac gcggctatta gatcaatgtg agaccacgag
      301 tgattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      361 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      421 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      481 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      541 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      601 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      661 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      721 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      781 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      841 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      901 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      961 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1021 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1081 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1141 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1201 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1261 ctaaagtata tatgagtaaa cttggtctga cagttatttg ccgactacct tggtgatctc
     1321 gccttttacg taatggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     1381 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     1441 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     1501 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     1561 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     1621 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     1681 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     1741 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     1801 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     1861 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     1921 ggtcaccgta accagcaaat caatatcgct gtgtggcttc aggccgccat ccactgcgga
     1981 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     2041 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt
     2101 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     2161 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaaa
     2221 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     2281 gttctggacc agttgcgtga gcgcatactc ttcctttttc aatattattg aagcatttat
     2341 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2401 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2461 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtccact gaagagccac
     2521 tgt
//
