LOCUS       sequence-495359-       16498 bp    DNA     circular SYN 07-JAN-2026
DEFINITION  Human eIF3k, eIF3l, eIF3e, eIF3f, eIF3h, eIF3d and eIF3m expression 
            in insect cells for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16498)
  AUTHORS   Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V
  TITLE     Purification and characterization of recombinant human translation 
            initiation factor eIF3.
  JOURNAL   Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388.
  PUBMED    41432235
REFERENCE   2  (bases 1 to 16498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 7, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2026-01"; volume: "35(1):e70388. doi"; pages: " 
            10.1002/pro.70388"
COMMENT     Sequence Label: pBIG2AB-eIF3klefhdm
FEATURES             Location/Qualifiers
     source          1..16498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(493..584)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    740..874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2143..2234)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    2329..2377
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     polyA_signal    2735..2869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4153..4244)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    4400..4534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6082..6173)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6329..6463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(8368..8459)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8615..8749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(9616..9707)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9802..9850
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    9948..9981
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(10052..10057,10058..10074,10075..10080)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             10269..10802
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  10869..11092
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(11227..11815)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11307..11326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11925..12027
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12028..12687
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12094..12113)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     mobile_element  12768..12933
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    13248..13382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(14717..14808)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    14964..15098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(15258..15284)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcactccaaa ctgtttctgg aacttcttct gcagcctgat cctctctctc tctttctgtt
       61 tagcgctctt agggaggatc tggaggttga attgcagcat gttcctcctg tccttgtctc
      121 tcctgagatt gcgctgggca aacctcatcc tgttgcgttg gtaagcggtc ttctgtgtcc
      181 tggcagtatc gacgagctgg aaagaggatt cgtcctcctc atgaaagtaa gcgtactggc
      241 ttcctcctcc aaactgggag ctgtacttgt tggtgtatct cttgtcttgg taggtggctc
      301 cagtccaatc tgcgacttta ccgagcctat cacccttgga gaatggttgg tagggcatgt
      361 ctctaaactg ttcaggcaca gcacaaggac cccaaccaga agggttgtct tggatcactg
      421 gagtcatgaa cttagccatg ttgactcgag ggatccgcgc ccgatggtgg gacggtatga
      481 ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac agtaaaatac
      541 ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta atattccgga
      601 gtaggtcgat ttaaacgtgc aatagtatcc agtttattta aatggttatg atagttattg
      661 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcttc
      721 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
      781 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      841 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      901 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      961 tggtatggct gattatgatc ctctagtact tctcgacaag cttgtcgaga ctgcaggctc
     1021 tagaggtacc ttaattgttg tattcttgga gtgcctgtgc catgaacagc ttaccgaggt
     1081 tctgggcagt gaactccttg atgttctgac aataggtatt gatctggcct gcgatcagca
     1141 gagagtccat tctagcaggt ggctgaggtg gcttgaacag cttggacaga tcctcttcag
     1201 ggagtggagg ttcgcctctg ctctggcgtt gcatgttctc ctgttgtctt ctttgttggt
     1261 attgatgttt ctgctgctgc tgcttggagg tattcctcat gtaggtgttg tacttcacga
     1321 tgtcttggct catttcatca accctgtcca tgaggagctg caggttcttt ccgagatggt
     1381 tggaggaagc gaggctcagc agttcatgct tgtcagcaac agcagacttc ttctccagct
     1441 cccacatcag gacgttgatg aggtgggagt tcttgataac gataggcacc tcttcaaaca
     1501 tgtactcgaa tgtaatgttt gccttcttga gagcttcagg gctgaagtcc ttctctttgc
     1561 agacctccat cagcttagga gtcaggcgat atgccttgag ggagagagat ccctgagcag
     1621 tcttgatggg atcgtagatc agcaccacag attcctcaat agcgtgttgg taagagaact
     1681 gagagtccag gagagcccta gtaacaaagg agccatagta tgtggattga taccatccca
     1741 catgcagatg gtcgatgttg acatgcctca ggctgcgcat catctccatt tgatactgga
     1801 cctcatcgaa gtcggcatca tcttcggtat gctgagggaa agggaaacag ttggtgatct
     1861 ccagtctatc ctcgaccacc agacccagga gcacaccctg cacaacttca gtaccttgac
     1921 cctcttcctg gtaatgcttg atgatcttca gcaccaccag accgtcgatt tggacctgct
     1981 taacagcgct gtcaccagaa cctcccttgc ctttaccttt accagcagct ccagctgtgg
     2041 aagagctgga agtagcagta gatcctgtac cctcctttct ggaagccatg ttgactcgag
     2101 ggatccgcgc ccgatggtgg gacggtatga ataatccgga atatttatag gtttttttat
     2161 tacaaaactg ttacgaaaac agtaaaatac ttatttattt gcgagatggt tatcatttta
     2221 attatctcca tgatctatta atattccgga gtaggtcgat ttaaatcttt agaccataga
     2281 gcgttcactt gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
     2341 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     2401 attgtctcct tccgtgtttc agttagcctc gataaaaacc ccgattgaga tatagttttt
     2461 aattaacccg tagtggctat ggcagggctt gccgccccga cgttggctgc gagccctggg
     2521 ccttcacccg aacttggggg ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc
     2581 caatggggtc tcggtggggt atcgactaac cccgattgag atatagattt atttaaatgg
     2641 ttatgatagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     2701 agcagccgga tcttctaggc tcaagcagtg atcagatcca gacatgataa gatacattga
     2761 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2821 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2881 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2941 aaacctctac aaatgtggta tggctgatta tgatcctcta gtacttctcg acaagcttgt
     3001 cgagactgca ggctctagag gtaccttaga gattgaccag cttctcgttc agtgcgatct
     3061 gggattgagt gagattggcg aggtaagtaa ccatgagcag gtcgttgatg ttggagttca
     3121 gcatggtttc gaagtcgtct ggaacgatct ttgggacttg gttaaccagg gacatgagga
     3181 agcgtcccac agtgttatct gcggacacct taccggacag cacatcctcg gcatactgca
     3241 ggacggtaga gagtgcgtcc tggattctgg cagaggctcc accaacttgc tgcagatcag
     3301 aggagagtcc gatgactctg tttgggctga agcaagtctt catgatcagg tccacgccaa
     3361 tcctttcggt gtcatagtaa gcgtacttca ctgtcagtgg agtgaacatc acacccattg
     3421 tcctgcctgg aactcccatc agtgtagaca cgtaggcctt gatgctcatg cgaccgttct
     3481 gcagggaggt gtccactgtc aggtggatag gatttggagc ctctctggag tagtattcat
     3541 gaatgagcac ggagtgctca gtgatgtcgt gtccagtggc gtaccaaccg aggatcagtt
     3601 cattagggct aactttctta tgcagttcgt acatgttctt tgcgaactcc atgtccacgg
     3661 caacctcgtc ttcgctttca ttgtgtggca cagagaaaca gttagtcacc tcgacagaat
     3721 gcttgtcaac tgtaccgagg agagtaccaa tcaccctagc agcaccttcg tttcttctct
     3781 cgtagctatc gacaatggaa gccaggataa caggatgcag gcgaaccacc cttcctcctg
     3841 ggaaaggtcc tggcagagct ggtccaggca gtgctggagc tggtgtctgt gcaggagcct
     3901 gagcggaagc tggtgtctga cctggtgcag cagttgcagc agcagcagca gcagggtcgg
     3961 aagagcttgc aggtgcagca gcagggacgg gagctgcagc aggagcagga gtaggagctg
     4021 gaacgctggc tggagctgct gcaggaacgg gagttggagt agctggtgga gcagacacag
     4081 gaacggctgg tgttgccatg ttgactcgag ggatccgcgc ccgatggtgg gacggtatga
     4141 ataatccgga atatttatag gtttttttat tacaaaactg ttacgaaaac agtaaaatac
     4201 ttatttattt gcgagatggt tatcatttta attatctcca tgatctatta atattccgga
     4261 gtaggtcgat ttaaactaag ctatgtgaac cgtttattta aatggttatg atagttattg
     4321 ctcagcggtg gcagcagcca actcagcttc ctttcgggct ttgttagcag ccggatcttc
     4381 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
     4441 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4501 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4561 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4621 tggtatggct gattatgatc ctctagtact tctcgacaag cttgtcgaga ctgcaggctc
     4681 tagaggtacc ttaatagaaa ccgctatctt gtgtagccca gttaggtgct tcggatctgg
     4741 agttctggtt cagcttcttc tcgatgttca tggcgagcat ctgagagcga aagctcaggg
     4801 acttggtctt ctcgataacc tgttggtatg gagacacggc attgttgccc atcaccacgt
     4861 gaccgagctt agagtcgatc ttagcgtcga gcctagcgtt gcgaatcaga ttcacgatcc
     4921 atctctcagc ctcttcaggt gtcatgttga gcttatcagc cagcatgttg atgctaatgc
     4981 actgatgaat cctgcagaag gtttcgaaga tgaagagcct ggcattctcg atgaagtcct
     5041 ccaggcaggc aaccaggaag aaatcgttca cgagaacaga ctcacactct ctcagcttct
     5101 tctgagcacc gtcgaagtcg aagttcacat acaggcattc cacaaactca gtgattgggt
     5161 ccttgtatgt ataggactcc tgctgaatca ccttgaccag gtccttgagg acctgtcttc
     5221 tcttcctcac gtccttgttg gtaatcacag cggttgtcag gtatctcagg atgtgtgggc
     5281 acatagtctg gatggcgttc aggtattgag gttggtacag aaacagatca atgatgttgt
     5341 cccttccctt gggatggttg aagaacacga acagggacca atggatgagc caggttcttt
     5401 gctggaggga ctgcagtgga gaagacacgc tattgttgtc aatggtttct ttgagtctgg
     5461 tgaggtcctc cattgcagca tcccagttct gcatcaggat ttcggaagcc agcttacccc
     5521 acagagaaga cagagcgttc ctgtctgtgg ctggcaccag cactctgaag aagtacaggt
     5581 attctgcagc tccagagtag ttgccacact cgtactggaa cttagcgtac ctgtacaggg
     5641 tgtcgaggta ctcctgcctg aagccgtgtt tgtctgccag atagtcaaac agcatgcgtc
     5701 cgtctctagt agactgcatc tgcctggttg tttcagggtc ttcaaacatc ttcacgatgg
     5761 gctcagtctc ggcttgcagt tgcttcagct gagcaacaac tgtagtcctc ttctccctga
     5821 gagcgtgagg aatgtcgtcg ctgtacaggt tcttgtacac atccattgcg aaatcgacca
     5881 tgttggtgtc gctcagcaga tccagtttac cctggaggag ttccttctca ttgtagatct
     5941 ccttcacgga gaggaactcc aggagaggaa acaccaggtg cctatccagg aaatgagcaa
     6001 tcctggttgt cagatcgtac tcagccatgt tgactcgagg gatccgcgcc cgatggtggg
     6061 acggtatgaa taatccggaa tatttatagg tttttttatt acaaaactgt tacgaaaaca
     6121 gtaaaatact tatttatttg cgagatggtt atcattttaa ttatctccat gatctattaa
     6181 tattccggag taggtcgatt taaacatcag gcatcattag gtttatttaa atggttatga
     6241 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     6301 cggatcttct aggctcaagc agtgatcaga tccagacatg ataagataca ttgatgagtt
     6361 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     6421 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     6481 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     6541 ctacaaatgt ggtatggctg attatgatcc tctagtactt ctcgacaagc ttgtcgagac
     6601 tgcaggctct agaggtacct tatggtcttt gtcccatctt cttgagtgtg cggttcagct
     6661 cttcaaactt gtgaatctgc ctgatgaaga agtctccgta cctcctggcc acctttgtat
     6721 cagcgatgtg gatcatgtcc ttgtcgatgt agaagtcaac ctcgcttgca gactgaaact
     6781 caccgtccag agcgctgatt ccggaagtcc acaccaggtt cttcatcttg tgcttgaaca
     6841 cgagcagctg aatgcgaaac tcctgttcag tcagatccag gaatcctgcc agcttagcca
     6901 caggcatagt tgtatagagt ttcaggaagc tgcggattgt agacagttga gcctgctgct
     6961 ggacctcgtc agagaacact ttcagctgtt gcaggaaagg ctctttgtga tagtttgggt
     7021 gcacgttgtc atagttaggg acgactggag agagaaactt tgggcaagaa tagctgaaca
     7081 gttcttcgta aacctgagga tcacccttct gcatcctgag catcttatct ccgtacttct
     7141 ctctcagttg gaggtggatg gattcatcaa ttctcatggg atacatagtc agggcaattg
     7201 ccaggagtgc gtgcatctgt tcgttctgct tgttgatcat ttcatacttg taagtggtcc
     7261 tttgaaacat ggatttggtc ctctggatgt acaggaggat gttagcgaag accctgatgg
     7321 catcctggta gcgtctcatc atgaggtagg cgaagcccac gtagtagtaa gtagtgacct
     7381 ggcactcagg gactctgcta tacatagact tcttgttgag ttcgatgttc tccagcactt
     7441 tgatagcctg gtagtagtct ccgagcagag agtggagcct gagcagaccc accagggaga
     7501 agtagcccag catcttgtag aggctatgtc taccgtactc accagcaacg ctctcaggat
     7561 caccaccgct tgtgtacact tccagctgcc tgttgatgtt ggatttgtcc accagggagt
     7621 gcagcacgtt gagcacagag tgaacattcc agatcttggg attgcttctc aggaaatcaa
     7681 tctcctcttc ggatttctta gcggtcttac atctgtactg agagaaggac tggaactgat
     7741 agatgaactc atcaatgatg tcccagagcc actggttggg cagttccaga ggtgcaggtc
     7801 catcggcatt cagaatgtag ttgaacagat tgcagtagtt gtagtagctc tcaaatcttt
     7861 gttccaggga gggtcctcca gaaaccttag cgtagatatg tctgtagtac agttccttgt
     7921 acagaatcag gaacacggca tcatttccca cctgtggagc gatagcttca gcctcaggcc
     7981 agggagtgtt cttgaagaat ctttcagtca gtttggtcca gctgttctca tagatgtcct
     8041 ggatctcata caccttctgg tcaatgacgt cggaagacac tctgcttgcc tgcagctcat
     8101 agaccttctg gtcgatgagg tcggacacgg tcttatggaa gtattgaatg aagttcttga
     8161 tcacctctgg gatcacttgg taggtttgct gttcgtattg tctctcgtat gccagatctt
     8221 gtttagggtc gcctgtgtgc atgtcgtaat cagaagggta ggcgtatgga tcataagcag
     8281 cctcagattc atagtcatcg gcaggatagg acatgttgac tcgagggatc cgcgcccgat
     8341 ggtgggacgg tatgaataat ccggaatatt tataggtttt tttattacaa aactgttacg
     8401 aaaacagtaa aatacttatt tatttgcgag atggttatca ttttaattat ctccatgatc
     8461 tattaatatt ccggagtagg tcgatttaaa cgtgcaatag tatccagttt atttaaatgg
     8521 ttatgatagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     8581 agcagccgga tcttctaggc tcaagcagtg atcagatcca gacatgataa gatacattga
     8641 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     8701 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     8761 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     8821 aaacctctac aaatgtggta tggctgatta tgatcctcta gtacttctcg acaagcttgt
     8881 cgagactgca ggctctagag gtaccttact ggctggaagc catgatggaa gacacgctat
     8941 cgaaatcaat cttctcgacg atgttctttg gtttgataga ctcttcttgg ctacagatga
     9001 agatctgacc gctctcatca gcggaccatc cgtacttgga catccacacc ttgagctggg
     9061 aatcgctcag atctcccagc atttcagcca gcagccagcg gtcgatgtgc tggtaggtga
     9121 ttcccaccac atggcagatg aacttgcgca cgctatcttc gaagccagtg ataccttcga
     9181 ggagatccat gttctcgtcc agggcttgcc agaaggcctg gaaatggcat gtttcgagga
     9241 gatcacccag gtagaggatt tgtctgattg gcctttcttc ttggtgggct tggtcgatca
     9301 tgcacttgca gagagtgaag tcggtgtgag gcaggtttgt cagggctttc aggaggatct
     9361 gagcagtcac ggttgtctga aagaatgcag ggttgaattg gtagagtttc agcacagcca
     9421 gattagcctc caggtcgtaa gcattctcct tggcttgtgt ttccacatag cgttcgagag
     9481 tggccagatt ctcgggattg tacctgtcaa tgcccttcag cagctttcca acattggctc
     9541 tcatttgctc aaacatagcc atgttgactc gagggatccg cgcccgatgg tgggacggta
     9601 tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa aacagtaaaa
     9661 tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatcta ttaatattcc
     9721 ggagtaggtc gatttaaatc tttagaccat agagcgttca cttgacagag tgccagccct
     9781 gggaccgaac cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt attctgtctt
     9841 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc
     9901 ctcgataaaa actttagacc atagagcgtt taattaacct aggccagata acttcgtata
     9961 atgtatgcta tacgaagtta tggcgacttc gctgctgccc aaggttgccg ggtgacgcac
    10021 accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg ttcgtaaact
    10081 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
    10141 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
    10201 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
    10261 cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
    10321 agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct gaccaagtca
    10381 aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta gccacctact
    10441 cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag acattcatcg
    10501 cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac gttctgccca
    10561 agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc ggcgagcacc
    10621 ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc aacgcgcttg
    10681 gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg gctctctata
    10741 caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt accgccacct
    10801 aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt aaattgtcac
    10861 aacgccgcga atatagtctt tacatgccct tggccacgcc cctctttaat acgacgggca
    10921 atttgcactt cagaaaatga agagtttgct ttagccataa caaaagtcca gtatgctttt
    10981 tcacagcata actggactga tttcagttta caactattct gtctagttta agactttatt
    11041 gtcatagttt agatctattt tgttcagttt aagactttat tgtccgccca cacccgctta
    11101 cgcagggcat ctgcgcatcc acaggaagag cgacccaagt caatcagggg ataacgcagg
    11161 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    11221 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    11281 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    11341 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    11401 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    11461 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    11521 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    11581 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    11641 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    11701 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    11761 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    11821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    11881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttgatcg gcacgtaaga
    11941 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
    12001 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
    12061 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
    12121 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
    12181 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
    12241 gagttccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
    12301 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
    12361 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
    12421 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
    12481 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
    12541 actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
    12601 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
    12661 tgcgatgagt ggcagggcgg ggcgtaataa attaaaaatg aagttttaaa tcaatctaaa
    12721 gtatatatga gtaaacttgg tctgacagtt gacccaagtc aaccggttgt gggcggacaa
    12781 aatagttggg aactgggagg ggtggaaatg gagtttttaa ggattattta gggaagagtg
    12841 acaaaataga tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta aaaatgacaa
    12901 aatagtttgg aactagattt cacttatctg gttcggatct cctgattgaa acgtgcaata
    12961 gtatccagtt tttaattaac ccgtagtggc tatggcaggg cttgccgccc cgacgttggc
    13021 tgcgagccct gggccttcac ccgaacttgg gggttggggt ggggaaaagg aagaaacgcg
    13081 ggcgtattgg tcccaatggg gtctcggtgg ggtatcgact aaccccgatt gagatataga
    13141 tttatttaaa tggttatgat agttattgct cagcggtggc agcagccaac tcagcttcct
    13201 ttcgggcttt gttagcagcc ggatcttcta ggctcaagca gtgatcagat ccagacatga
    13261 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
    13321 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
    13381 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
    13441 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagtacttc
    13501 tcgacaagct tgtcgagact gcaggctcta gaggtacctt aggtgtcaga gaggctcagg
    13561 agagagttct tcaccttgtt gaggttctgc ttccaagcgt tcagagtgtc atacagctgt
    13621 tgccactgct gcttaccgaa tgttctgtga gtgctgtgag acacgacgac tttgcgctga
    13681 gtctgatcaa tcttgcagta aaccatctta gttctaactg cgtcgatgac gaaagcctcc
    13741 acatcatctg ctccgatctg gagttcctgt tgcatagtgt cgaagctaat ctccttgttc
    13801 tcaacagcca ttcccatgaa ggtcagcagt ctcatcttgg ccatgttctg ttcatgcagc
    13861 agtcccaggg agtcgatgaa gtccttgttg ttctggtaga acttgacgta agaggcgagc
    13921 ttagcgctca cgaagatggt caggagatca tggatcagtt cgccttccag aaacttcacg
    13981 ggtttgaggg tcaggaggtg gtcgaacagg aaagcatttg ggtccttcag ggctctaaca
    14041 atgcacctat gagcatcgac cctggcctgg gaagcattgt cctcagtgta ggaacccagc
    14101 agctccacca tcactttaga ggcagcgtcg ctcttcttgc agtccaccag agcttcatac
    14161 agcagcctca gcagggtgtg tttcttctca gtagtgaggt tccagtcaga gatccatttg
    14221 cggacttggt ccagttcggt tggaatgtac tggatagcac cgcagcttgc agcaaccttg
    14281 atgagagaac agtaaacggt gtacctgact ggagtgttct tgtccattcc atgaaacagg
    14341 ttggagagga gctggagcct gaggctaggt ctttctccct ccctgaactt cacgagcttc
    14401 tcgcacaggg actcgatgag agcttcttgc ttatcgggtt cgaggatcag gaggagggac
    14461 acaacgctat tcataacgct ttccacatcc ttgtcgtcct ccttcaggca cacatcgcat
    14521 gcctcgatga tctgagcgag atcgacgtgc aggcctccct cgctgttctc ctcagagatt
    14581 tcagctccct tgctcttcag ataagccctc agttcggctg cttggtcttc ttcagagatg
    14641 tcgatgaaag cagggacgct catgttgact cgagggatcc gcgcccgatg gtgggacggt
    14701 atgaataatc cggaatattt ataggttttt ttattacaaa actgttacga aaacagtaaa
    14761 atacttattt atttgcgaga tggttatcat tttaattatc tccatgatct attaatattc
    14821 cggagtaggt cgatttaaac atcaggcatc attaggttta tttaaatggt tatgatagtt
    14881 attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat
    14941 cttctaggct caagcagtga tcagatccag acatgataag atacattgat gagtttggac
    15001 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
    15061 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
    15121 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
    15181 aatgtggtat ggctgattat gatcctctag tacttctcga caagcttgtc gagactgcag
    15241 gctctagagg taccttaggc gtaatcaggg acgtcataag ggtaggagga gcctgtctcc
    15301 tcttcctcct cctcttcttc ctcttcttct tcctcctctt cgtcctcatc ggaagagaag
    15361 gtgccgtctg ggagagagta caccctaatc acctgtttgt ttggatcttt caggatcagg
    15421 tacttgcctt cttcgagttt catgcagatg tcgataacgc atctcaggat accccaagcg
    15481 ttctcgacgc tgaggttgat ctggctggcg aactcgttag gcttgaactg ctgggtgccg
    15541 aggatcacat gtctgctgct atccttcaca tggtagcgag aaacataacc cagcttcagg
    15601 tattcgcttc cagcgagcag agcacaacaa gtccagcgag ccagtttgta ggagttgttc
    15661 ttcagctctg tagcgataac agcacctctc tggctatcga gtttctgtct ccagtccaca
    15721 ccattgcagt gcctagaatc ccactcgttc agtgtcttga tgttgatgaa gctaacctca
    15781 ccgtttgcac ctgtcatcac tccgtcatgt tcgcacctaa cgatcaggtc aatgtcgtct
    15841 cccagcttcc agcgtctata gcgataagca acggaggcga tctcgttctt gtccatatcg
    15901 tcttcaacga aaggattggg atttggaaag ttgtacctct ccttacccat gcggagacac
    15961 tgttgggaga agttgtggtt gatgtaagta gcctccatag ccagattgcg tgggctgttg
    16021 aagctgttgc cttcatcttg aggtggttcg ttggcagtct cggagacggt caggagatcg
    16081 aaatcggaat tgtccctctt atcgaagaac agtttagatc ccactctctg caccacaatg
    16141 tcccaggaat agacagacct ggtgcagctc atcagagtgg ccaggatagc atcagtagcg
    16201 aagacgtttc cttgagtctt agccagcttg cgaatgactg ggtcgtcagt tgttgtcaca
    16261 gtgtggaaga ttctcttgat ggagcgcagt ggcttctcgg agcgagtagt aatcctgtcg
    16321 aaggccttat cgtagtactc cagagcacca caacactcga tgtcttgtgg ttcggacact
    16381 tccaggtatc tcatcttcat cagctgaggg aagtccattt cttccttaac ctcccaatcg
    16441 ctgcggacct caacggagga atcccttggc ttctgggact tctgatccca tttctgcc
//
