LOCUS       sequence-495375-        7441 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  mScarlet-I fluorescent reporter for synthetic Mesorhizobium nifH 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7441)
  TITLE     Fluorescent reporters for rhizobium-legume symbiosis 
            [BIORXIV/2025/681774]
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNDMS239
FEATURES             Location/Qualifiers
     source          1..7441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            140..248
                     /note="incP origin of transfer"
     CDS             complement(259..921)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      922..1693
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(4202..4288)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5383..5477
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5486..6136)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             6242..7441
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 actacttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
       61 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
      121 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
      181 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
      241 gctggccgta ggccggccct accggcgcgg cagcgttacc cgtgtcggcg gctccaacgg
      301 ctcgccatcg tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg
      361 ttcccattcc tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg
      421 ctggctgggc ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt
      481 cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac
      541 caccacggcg gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac
      601 gcggtcattg accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat
      661 ccagcgctcg gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat
      721 gagcttggaa agagtctttt ggctgaccac cacggcgttc tggtggccca tctgcgcgac
      781 gaggtgatgc agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc
      841 atgcgctttg cgttccgttt ggacccagtg accgggcttg ttcttggctt gaatgccgat
      901 ttctctggac tgcgtggcca tgcttatctc catgcggtag gggtgccgca cggttgcggc
      961 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
     1021 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
     1081 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
     1141 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
     1201 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
     1261 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
     1321 aggctcgggg ggcaggcggg cgggcttcgc ccttcgactg cccccactcg cataggcttg
     1381 ggtcgttcca ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg
     1441 ccttcctctt ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc
     1501 cccagagaaa attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt tggagccggt
     1561 gggtatgtgg tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca
     1621 ggcgcagcct gtccatcagc ttgtccagca gggttgtcca cgggccgagc gaagcgagcc
     1681 agccggtggc cgctcgcggc catcgtccac atatccacgg gctggcaagg gagcgcagcg
     1741 accgcgcagg gcgaagcccg gagagcaagc ccgtaggggg ttactgcatg agcttgtgga
     1801 agtgtgctga gcataaatga ttctcctagc tgttctttgg gtaagtacgc catcaggacg
     1861 ttgtgagtgg cgcgattttt agcggctgaa atcagccctt gagcctgtcg gcaagtcgcg
     1921 tcatgaggtc catgcgctca tgcaggatcg ccacgaccaa cgcgggttcg cccgcacgcg
     1981 gcaggcaaaa aacgtagtgg tgttcgcagc gggccatccg cagcgcggga aagagttcgc
     2041 tcatgtcctt aaacgggcct tcgccggcgg caagcctggc tatgccctgt tccagcttag
     2101 cgatatagcg gcgcacctgc gccgcgcccc actcccggcg cgtgtagcgg atgatgccgc
     2161 gtagatcggc ttcggcctca gccgtgagga tgtaggccgt caagcgcgat ccccgctgag
     2221 ttcttcatca agaatttcgc cgacgctctt ggtggacacc ttgccggcaa gcccatcgtt
     2281 gatgcggttc cccagcatgg ttttcagttc ctgccatgcc tgatcggcat cagcgtcacc
     2341 ggggaacaga cgttcgaggg cgtattgctt aatggtcttg ccctgcaagg cggccagggc
     2401 tttcaggctc tggtgctgct ggtccgtcat gtcgattgtc aggcggctca ttggataacc
     2461 tccataaaat acacgtaacc acattagcac atatgtgggc gtgaggctac agcgcgaggc
     2521 gcattaaggt cgggaaaatg cgctaggcgc atttaaattg cgtattgctg taatgcgcca
     2581 tgccggctag actaggccca aatgggtata cccaatttga ccaaggggga cgcgatgagg
     2641 gcggccaagc actaccgaca acttctatcc atcgacttca acatcgaggc gctggccttc
     2701 gtgcctggac ccgacggcac acgcggccgg cgcatccacg tcctggggcg cgaggtccgc
     2761 gaccggcccg gcctggtcga gtacctttcg ccggcgttcg gctcgcgggt ggcgctggac
     2821 ggctactgca aggccaattt cgatgcagtg ctgcacctgg cgtaccccga tcatcagcaa
     2881 tggggccacg catgaagcgc cgaagctacg ccatgctgcg cgccgctgcc gcgctggccg
     2941 tcctggtcgt tgcctcgccg gcatgggccg agctgcgcgg cgaggtcgtg cgcatcatcg
     3001 acggcgacac catcgacgtg ctggtagaca agcagccggt gcgcgtgcgc ctggtggaca
     3061 ttgacgcgcc ggaaaagcgg caagccttcg gcgaacgtgc gcgccaggcg ctggccggca
     3121 tggtgttccg ccggcacgtc ctggtcgacg agaaggacac cgaccgttac ggccgcacgc
     3181 tgggcaccgt ctgggtcaac atggagctgg ccagccggcc gccgcagccg cgcaacgtca
     3241 acgccgcgat ggttcaccag ggcatggcgt gggcctatcg cttccacggc cgcgcggccg
     3301 accctgaaat gctgcggctc gaacaggagg cgcgaggcaa gcgcgtcggc ctctggtccg
     3361 atccgcacgc cgtcgagccg tggaaatggc gacgcgagag caacaaccgg agggacgaag
     3421 gttgaaggtc gcccgcatct acctgcgcgc cagtacggac gagcagaatc ttgaacgcca
     3481 ggagagcctt gtagcggcca cgcgggccgc cgggtactac gtcgccggca tctaccgcga
     3541 gaaggcgtcc ggcgcacgcg ccgaccggcc cgagctgctg cgcatgatcg cggacctgca
     3601 acctggtgaa gtcgtcgttg cggagaagat cgaccgcatc agccgcttgc cgttggccga
     3661 ggccgagcgc ctggttgcgt cgatccgggc caaaggggcc aagctggccg tgcctggcgt
     3721 ggtggacctg tcggagctgg ccgccgaggc gaacggagtg gcgaaaatcg ttctggaatc
     3781 cgtccaggac atgcttttga agctcgcctt gcagatggcc cgcgacgact acgaggatcg
     3841 gcgcgagcgt caacgtcagg gtgtccagtt ggcgaaggcc gccggccgct acaccggccg
     3901 caaacgtgac gccggcatgc acgaccgcat catcacgctt cgctccggcg gatcgagcat
     3961 tgccaagacg gccaagctgg tcggatgcag cccgagccag gtcaaacgag tgtgggcggc
     4021 ctggaacgcg cagcagcaaa aataaagccg ggcagtgccc ggcttttctc accttttcgc
     4081 gtcccgcagg gccgctgcga gcgccctacc tagatcctcg ctttccccct cggtgtagtc
     4141 cggccagggc acgaagggcg cggatgcgaa cctgttgagc aggtcagatc tagggcggcg
     4201 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4261 ctttcgactg agcctttcgt tttatttgat gcctgccgaa ttcggatccg gagcgcagcc
     4321 aaaaacctgg tctgacttag gaccatccgc ttgagctcga ctctcgctgg atggcacccg
     4381 acgttttgtg tcggctttgt cgggtccgct acacaggctt ttatgggccc gatcacttgt
     4441 tttgccacaa gctaactagc tggaaagtaa tgaaaaaatt tccttttggt atcggccggt
     4501 caagttggca cgagtcttga gatccttggt gcgaggcggc aagagctgcc catcggcatt
     4561 tatgtcgcgg gcagccgcga tgataagaac aaggaaggaa aaactataat ggtcagtaaa
     4621 ggcgaagcag ttatcaaaga gttcatgcgc ttcaaagttc atatggaagg gtcgatgaac
     4681 gggcacgaat ttgaaattga aggcgaaggc gaaggccgcc catatgaagg gacccaaacc
     4741 gcaaagctta aggttactaa aggcggtcca ttaccctttt cgtgggacat tttaagccca
     4801 cagtttatgt acgggagtcg cgctttcatc aagcaccctg cggacatccc agattactac
     4861 aaacagtctt tccccgaggg gttcaagtgg gagcgcgtga tgaacttcga ggatggcgga
     4921 gccgtgacgg tcacccaaga tacctctttg gaggacggta cgttgatcta caaagtgaaa
     4981 ttgcgtggca cgaattttcc acctgatggg cctgtcatgc agaaaaagac aatgggatgg
     5041 gaagcttcca cggagcgcct ttacccagag gacggtgttc ttaaagggga tatcaaaatg
     5101 gcgctgcgtc ttaaagatgg aggccgctac ctggcggact tcaagactac ttacaaggcc
     5161 aaaaaaccag tgcagatgcc gggtgcgtac aatgtagatc gtaaattaga tattacaagt
     5221 cacaatgaag attacacggt cgtagagcag tatgagcgca gtgaggggcg tcactctacg
     5281 ggcggtatgg acgagttata caagtaaagg tctaacgcat gagaaagccc ccggaagatc
     5341 accttccggg ggctttttta ttgcgccctt gagacgctct tggactcctg ttgatagatc
     5401 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
     5461 tttttattgg tgagaatcca ggcaatcaat cgtcaccctt tctcggtcct tcaacgttcc
     5521 tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc tgctcgaacg
     5581 ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc ggcgagagcg
     5641 gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc tgctcctcaa
     5701 gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg tccccggccg
     5761 aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc tgcggtgcgc
     5821 ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc gcctgcctga
     5881 agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc ggcaccgaat
     5941 gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc cgcttgttcc
     6001 tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt ttgcgtgtcg
     6061 tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc actgtattcg
     6121 gctgcaactt tgtcatgctt gacactttat cactgataaa cataatatgt ccaccaactt
     6181 atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc ggaggccaga
     6241 cgtgaaaccc aacatacccc tgatcgtaat tctgagcact gtcgcgctcg acgctgtcgg
     6301 catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc actcgaacga
     6361 cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttggtgcaat ttgcctgcgc
     6421 acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc tcgtgtcgct
     6481 ggccggcgcc actgtcgact acgccatcat ggcgacagcg cctttccttt gggttctcta
     6541 tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg gcgcttatat
     6601 tgccgatatc actgatggcg atgagcgcgc gcggcacttc ggcttcatga gcgcctgttt
     6661 cgggttcggg atggtcgcgg gacctgtgct cggtgggctg atgggcggtt tctcccccca
     6721 cgctccgttc ttcgccgcgg cagccttgaa cggcctcaat ttcctgacgg gctgtttcct
     6781 tttgccggag tcgcacaaag gcgaacgccg gccgttacgc cgggaggctc tcaacccgct
     6841 cgcttcgttc cggtgggccc ggggcatgac cgtcgtcgcc gccctgatgg cggtgttctt
     6901 catcatgcaa cttgtcggac aggtgccggc cgcgctttgg gtcattttcg gcgaggatcg
     6961 ctttcactgg gacgcgacca cgatcggcat ttcgcttgcc gcatttggca ttctgcattc
     7021 actcgcccag gcaatgatca ccggccctgt agccgcccgg ctcggcgaaa ggcgggcact
     7081 catgctcgga atgattgccg acggcacagg ctacatcctg cttgccttcg cgacacgggg
     7141 atggatggcg ttcccgatca tggtcctgct tgcttcgggt ggcatcggaa tgccggcgct
     7201 gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag gggcagctgc aaggctcact
     7261 ggcggcgctc accagcctga cctcgatcgt cggacccctc ctcttcacgg cgatctatgc
     7321 ggcttctata acaacgtgga acgggtgggc atggattgca ggcgctgccc tctacttgct
     7381 ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca gggcaacgag ccgatcgctg
     7441 a
//
