LOCUS       sequence-495379-        8396 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  A CAGGS driven expression vector containing FastFUCCI to label cell 
            cycle state followed by an IRES and a membrane targeted mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)
FEATURES             Location/Qualifiers
     source          1..8396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(96..115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      909..1497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1398..1417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1651..1668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1736..1870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1760..1779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1814..1833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(1876..2071)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1890..2025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2130..2151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2166..2171,2172..2189,2190..2196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2204..2220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2209..2231
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2228..2244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2228..2244
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2587..2606
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2605..2660
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2706..2725
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2811..3512)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    join(3573..3581,3582..3584,3585..4159)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3746..3765)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     3975..3992
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4171..4188
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4197..4221
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4251..4580)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(4611..5288)
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             complement(4611..5288)
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             complement(5295..5348)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5355..5627)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(5682..6332)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     protein_bind    complement(6374..6398)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6439..6458)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6466..7482
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6520..6542)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7483..7757)
                     /label=chicken beta-actin promoter
     enhancer        7759..8138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8169..8273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8274..8342,8343..8396,1..738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc
     1741 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1801 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1861 taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     1921 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     1981 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     2041 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     2101 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2161 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2221 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     2281 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     2341 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     2401 aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc
     2461 agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag
     2521 catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
     2581 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat
     2641 gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc
     2701 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccaggg cattggccac
     2761 accagccacc accttctgat aggcagcctg cacctgagga gtgcggccgc ttaattaagc
     2821 ttgtgcccca gtttgctagg gaggtcgcag tatctggcca ctgccacctc gtgctgctcg
     2881 acgtaggtct cgttgttggc ctccttgatt ctttccagtc tgtagtccac atagtagacg
     2941 ccaggcatct tgaggttctt agcgggtttc ttggatctat atgtggtctt gatgtttgcg
     3001 atcagatggc tcccgcccac gagcttcagg gccatgtcgt ttctgccttc caggccgccg
     3061 tcagcggggt acagcgtctc ggtgaaggcc tcccagccga gtgttttctt ctgcatcaca
     3121 gggccgttgg atgtgaagtt cacccctctg atcttgacgt tgtagatgag gcagccgtcc
     3181 tggaggctgg tgtcctgggt agcggtcagc acgcccccgt cttcgtatgt ggtgactctc
     3241 tcccatgtga agccctcagg gaaggactgc ttgaagaagt cggggatgcc ctgggtgtgg
     3301 ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca ggatgtcgaa ggcgaagggg
     3361 agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta gggcttgcct
     3421 tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg tgccctccat gtacagcttc
     3481 atgtgcatgt tctccttaat cagctcgctc atgatctttt ggtcctcatc attcttttca
     3541 acctgtttgg ttcttctcat acagcacagc atggttgtgg ccatattatc atcgtgtttt
     3601 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     3661 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     3721 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     3781 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     3841 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     3901 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     3961 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     4021 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     4081 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     4141 ggggggggag ggagaggggg gggcggaatt atttaggtga cactatagga tatcgaacca
     4201 ctttgtacaa gaaagctggg ttgactggcg gccgctcagt cagatctcta cagggccttc
     4261 cgccgcttct cggccacctc tttccagtac tggctgctgg ggttttcttt gatcatcagg
     4321 tcgaagcttt cttgggtcac gccgcccagg ttcttgttct cgctgctctc gggcacgatc
     4381 acgcctgggc tagaggtggt gctggtcagg tggtcgttcc ggtgcttccg cttgctcagg
     4441 ccggcagaca gctcattctc ccggcccacc agagatccgc tggcgctggg ctggatcatc
     4501 ttcagggtcc gcctgggcac gctgctgttc ttgatgttct ctttgatctc ttcctgcttc
     4561 tgcttcatgc tggggttcat ttccagaggc cgccagtggc tggggtagcc cttggcctgg
     4621 ctgggcagca tgctgtacct ggccacggcg ttctcgtaca gcttcacttt gttgtagtcc
     4681 ttgtcgtgct tcaggatctc gatccggtgg tcgatcttgt gggcgtcggg cagccgcact
     4741 tctttcttgg ccttgtaggt ggtcttgaag tcgcatctgt agtgtccgcc gccttccagc
     4801 agcagccgca tgttcacgtc gcccttcagc acgccgtcct ccacgtacat cttctcggtg
     4861 ctgggctccc acttcagggt ctttttctgc atcacggggc cgttgggggg gaagttggtg
     4921 ccgtcgaatc tgatgtcgta gaaaaagcag tcgcccctca tgctgatgtt ggaggtggcg
     4981 gtgcagatgc cctggtcctc gtaggtcatg ctccgctccc agtggtagcc ctcggggaag
     5041 gtctgcttga agtagtcctg gatgtcggcg gggtacttgg tgaaggcccg gttgccgtac
     5101 tggaacacgg tggtcaggat gtcgtaggcg aagggcagag gggcgccttc ggtcacgttc
     5161 aggtccagga tctgggtgcc ctcgtagggg tttcccttgc cctcgccctc gatcacgaag
     5221 ttgtggccgt tcacggttcc ccgcatgcac agcttgatct tcatctcggg cttgatcacg
     5281 gacaccatgg atctggggcc ggggttctcc tccacgtcgc cgcaggtcag caggctgccc
     5341 ctgccctcgt cgacgatggt gtcctggtcc tgggcgctgg tcaggtgagg aggctgtccg
     5401 gcggcaggtg tgctcttctt gattttctgg ccagggctgg gacaggcagg gatatcgggg
     5461 gcctcggggg tgctagggct ggacacctca tccacggaca gccggagccg tctgcgggca
     5521 gggggcctgg cctgatctct gccaggggcg gcaggaggtc tggctctctt tctgctgccg
     5581 gaggtggcgg aggcaggggc tctcagggca ggtctggcag gagagggttc caggggcctc
     5641 caatggctag ggtagccgcc gaggctaggc agcatgctgt agtgggccac ggcatcctcc
     5701 acctgctcgg tgatgttgcc ctcggttttc cgcaccagcc ggtggccgat gtagtggtcg
     5761 ccgggcattt ccaggatttc tttggcggcc ttgtaggtgg ttttcatctg gcacttgtgg
     5821 tttccgccgc cttccagctt caggtacatg gtcacgtcgc ccttcagcac gccgtcgctg
     5881 gcggtgatct tctcggtgct gggctcccag tccacgctct ggttctgcat gatggggccg
     5941 tcggcgggga agttcacgcc ggtgaacttg ctcttgtggt agaaggtgtt gccccgcagg
     6001 ctgatgtggg cagacacaga ggcgctgccg ccatcctcga attccaggct ccgctcccag
     6061 ctcaggccct cggggaaggc ctgcttgaag tagtcgggga tttcctcggg gtacttggtg
     6121 aacacccggt ggccgtagca gaacacgtgg gacaccaggt cgaaggcgaa gggcatgggt
     6181 ccgccctcgg ccatggtgac tctcagggtc atttcctggt ggccctcgta aggtctgccg
     6241 gtgccctcgc cctcgatggt gaactcgtgg ccgttcacgc tgccgtccat gtagtaccgc
     6301 atcttcatct cgggcttgat cacggacacc atggatccca attcttaatt aatcacgagg
     6361 taccagtcag accagcctgc ttttttgtac aaacttgttt gatatctcga ggaattcttt
     6421 gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta ggaaaaagaa
     6481 gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc agaagccgaa
     6541 ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc cccgaggctg
     6601 gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt
     6661 cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc
     6721 agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct cgcacgatta
     6781 ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc tccgggggcc
     6841 gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc ccggcacccc
     6901 cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg tacggagccc
     6961 cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg ggggttgcag
     7021 cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc gcacgcagcc
     7081 tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg cgctcccctc
     7141 gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac agccgccggg
     7201 cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac cccccgagcc
     7261 gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca gccacagaaa
     7321 agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag ggccgtcccg
     7381 cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc gcgaggcggc
     7441 ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc gcgcgcttcg
     7501 ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc
     7561 gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct cgccccgccc
     7621 cgccccgcct ggcgcgcgcc cccccccccc cgcccccatc gctgcacaaa ataattaaaa
     7681 aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag tgaagcagaa
     7741 cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     7801 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     7861 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     7921 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     7981 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     8041 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     8101 aattgattac tattaataac tagtcaataa tcaatgtcga cccaggtggc acttttcggg
     8161 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8221 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8281 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8341 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacga
//
