LOCUS       sequence-49538-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, 
            Uney JB
  TITLE     Increased utility in the CNS of a powerful neuron-specific 
            tetracycline-regulatable adenoviral system developed using a 
            post-transcriptional enhancer.
  JOURNAL   J Gene Med. 2005 May;7(5):576-83.
  PUBMED    15580589
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene 
            Med."; date: "2005-05"; volume: "7(5)"; pages: "576-83"
COMMENT     Sequence Label: pXCX.CMV.tTA.WPRE
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..108
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     506..526
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(685..918)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag
       61 agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca
      121 gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccatg gaaaggacgt
      181 cagcttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca
      241 gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt
      301 cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa
      361 cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag
      421 caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata
      481 caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc
      541 agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacggatc ctctagatgc
      601 atgctcgagc ggccgccagt gtgatggaat ctgcagaatt ccagcacact ggcggccgtt
      661 actagtggat cctcgcgccc cctacccacc gtactcgtca attccaaggg catcggtaaa
      721 catctgctca aactcgaagt cggccatatc cagagcgccg tagggggcgg agtcgtgggg
      781 ggtaaatccc ggacccgggg aatccccgtc ccccaacatg tccagatcga aatcgtctag
      841 cgcgtcggca tgcgccatcg ccacgtcctc gccgtctaag tggagctcgt cccccaggct
      901 gacatcggtc gggggggccg tcgacagtct gcgcgt
//
