LOCUS       sequence-495381-        3983 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Level 0 Golden Gate part pAt1: Tip Delta (AT3g16240) promoter, GGAG 
            - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3983)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12030
FEATURES             Location/Qualifiers
     source          1..3983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2610..2628)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2696..2800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3022..3813
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 tagacgtata tgacaattaa ttaatgatcc gatccaaacg acccttggcg tatttggtaa
      901 ggtagcagtg atgtgcttaa ttattaacca acgatgtact gagtttctag aattagtaag
      961 aaccataaga gttttaaaaa gtgtaaaacg taaaacatca acacatgtca ttccagttaa
     1021 catacatata tctatatata tgttaacgtg tttgcataat ttttttaatt agagaaaaac
     1081 agcacactat gcaaaagatc gttcatatat ctaacgtgtg atttaatcga tctaacaact
     1141 ttttattccc ttttatatta ccaaaatatt tatttccatt gatcatatat ttgaagtagc
     1201 aaaaattggt aaaattttag ttaaaaatga gggagatgaa aactgatata ttatgtaaat
     1261 ctcaagtttt tgtaatgcat aagaaaaatg ttatcaccat tgcaactatc catggtagca
     1321 accgtatatc tcagcatatg atacaaaaaa aaaacaagaa gaagctcata ttcaaatttc
     1381 ttacgttmaa acgaataact tgattgctaa acaacaaatc tagatagcat tggattcatc
     1441 agtaagagtt aaaatcgtac agcattgtga atcataaaat gttgtacaag attgtgaaat
     1501 aacaaataat attgtaaatt tatcaaaaaa aaaggggatt gtggatcaat gaatatgatg
     1561 gagaatgttc atagaagagg taaataatag agggacccaa aataacattg ttgaaatgga
     1621 atataatcat aaatgagtca catcttcaaa atctgatcaa atcacttgga aaaaaaaaga
     1681 tcaagtcaca tgcaaccagt aaccacaatc atattttggc cggtctatca ccatacacat
     1741 tgtttacagc tcacacttgt ccctccttat atttgttcgt atccatttgt tttcttctct
     1801 ctctactcta atctcatctt tatgtttatc cctttttgaa ttttgaagct actctgtcct
     1861 ttgggtccac cctccaccac ctctaatttc aatcacgtta aaccggccat attactttaa
     1921 accgaccgtt ttctactaat aggaaaatcc gacccaaatt tttgactaat tataagacat
     1981 accgccgcta taccatgata ttgtcaaaga aaaagtttct aaccaataac cactatagac
     2041 taaaagattt caaataattt gattgatact gcattgaaca ttctacaatt ctacttatat
     2101 tgtgtagttc aaataatgag aacatatcgg aaagactagc cgctattaga ttatgaatgc
     2161 gtatgaatac attatacatt atagtatata tatatatata cacatatatt aaaatgtatt
     2221 actatataat aatttgtctt agtatagtac tcaaagaaat agagttttct tacgtagatg
     2281 tcatatattt tagtcatttt aggcaccttc ctaactcaaa tcacgaatag aacttatgta
     2341 gcaaaatgta aacacatgaa atcatttatt aattcgggtc caacgtaacc caccacacca
     2401 cagaaactca ttaaatagta tttttttata aaacttaagt tatatgcatg gatacatgaa
     2461 cgatactcct atataaagag aacagcattc aaaaggtctt atcatcttct tcactaaaca
     2521 aaaaaaaacc cttcaaaaca tttccttatt ctttcttctt catctacaac aatgtgagac
     2581 cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2641 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     2821 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     2881 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     2941 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     3001 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     3061 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     3121 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     3181 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     3241 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     3301 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     3361 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     3421 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     3481 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     3541 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     3601 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     3661 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     3721 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     3781 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     3841 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     3901 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3961 gtagaaaaga tcaaaggatc ttc
//
