LOCUS       sequence-495387-        3328 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Level 0 Golden Gate part 35Sx2+OsMac3 5' UTR promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3328)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12040
FEATURES             Location/Qualifiers
     source          1..3328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1245..1590
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1533..1553
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1955..1973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2041..2145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2367..3158
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca
      901 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga
      961 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
     1021 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt
     1081 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc
     1141 acgtctacaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctggtctac
     1201 tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa
     1261 aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
     1321 aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct
     1381 atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     1441 atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc
     1501 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1561 taaggaagtt catttcattt ggagaggaca cgctactgaa gcgccagaca aattcgtctc
     1621 cctcgttgga agtggaggcg cggaggcgga agcttttcct cctcctctcc tctcctctct
     1681 accctaggcg cgatctgctc gtccgcctcg gcgatccaca gggaaggagc agcatgctcc
     1741 acaaagacgc actacagcag actaaagaga gctttttcat accaaagaag tacaacaaaa
     1801 gatttgctcc tcattttctg aatcctggga ctctctagcc tgtagaagaa gaaaggcagg
     1861 aatttcagct caagagaaca gatcacaata tttacccacg gcactgtctc gcaataatgt
     1921 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     1981 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     2041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     2161 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     2221 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2281 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2341 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     2401 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2461 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     2521 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2581 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2641 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2701 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2761 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2821 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2881 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2941 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     3001 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     3061 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     3121 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     3181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3301 accccgtaga aaagatcaaa ggatcttc
//
