LOCUS       sequence-495390-        3103 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Level 0 Golden Gate part Act2 +SSU301 Leader promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3103)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12063
FEATURES             Location/Qualifiers
     source          1..3103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1730..1748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1816..1920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2142..2933
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 tcgacaaaat ttagaacgaa cttaattatg atctcaaata cattgataca tatctcatct
      901 agatctaggt tatcattatg taagaaagtt ttgacgaata tggcacgaca aaatggctag
      961 actcgatgta attggtatct caactcaaca ttatacttat accaaacatt agttagacaa
     1021 aatttaaaca actatttttt atgtatgcaa gagtcagcat atgtataatt gattcagaat
     1081 cgttttgacg agttcggatg tagtagtagc cattatttaa tgtacatact aatcgtgaat
     1141 agtgaatatg atgaaacatt gtatcttatt gtataaatat ccataaacac atcatgaaag
     1201 acactttctt tcacggtctg aattaattat gatacaattc taatagaaaa cgaattaaat
     1261 tacgttgaat tgtatgaaat ctaattgaac aagccaacca cgacgacgac taacgttgcc
     1321 tggattgact cggtttaagt taaccactaa aaaaacggag ctgtcatgta acacgcggat
     1381 cgagcaggtc acagtcatga agccatcaaa gcaaaagaac taatccaagg gctgagatga
     1441 ttaattagtt taaaaattag ttaacacgag ggaaaaggct gtctgacagc caggtcacgt
     1501 tatctttacc tgtggtcgaa atgattcgtg tctgtcgatt ttaattattt ttttgaaagg
     1561 ccgaaaataa agttgtaaga gataaacccg cctatataaa ttcatatatt ttcctctccg
     1621 ctttgaatac taaagtcagt gtgaagtgtt taaaggaaaa agctttggaa gaagcaaaaa
     1681 tcttctaact aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata
     1741 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1801 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1861 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1921 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     1981 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat
     2041 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2101 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     2161 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2221 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg
     2281 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2341 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2401 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2461 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2521 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     2581 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     2641 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     2701 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     2761 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     2821 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     2881 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag
     2941 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
