LOCUS       sequence-495399-        9379 bp    DNA     circular SYN 16-DEC-2025
DEFINITION  Constructs used for LplA-catalyzed labeling in mammalian cells. LplA
            is expressed as a fusion with mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  TITLE     Ting Lab LplA Plasmids 2025
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: His6-mCherry-Flag-LplAW37V and L172A
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    143..264
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    273..861
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             379..390
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     788..808
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1901..1924)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1934..2641)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2209..2228)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2478..2496
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(2654..2671)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      2683..2692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2711..2730)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2712..2730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2775..2978)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2804..2824)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2979..3358
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3530..3549
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3624..3728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3729..3797,3798..4589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3947..3966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4760..5348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5249..5268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5502..5519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5636..5657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5672..5677,5678..5695,5696..5702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5710..5726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5715..5737
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5734..5750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5734..5750
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5799..5920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6094..6888)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6205..6224)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6815..6834
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6955..7284)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6969..7104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7023..7042)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7269..7289
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7298..7726)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7408..7429)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7620..7639
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7772..7996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7985..8002
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8022..8040
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8022..8039
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        8068..8568
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8487..8505
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      8577..8586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    join(9308..9379,1..136)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
       61 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
      121 gcaggcatgc tggggacaat tgtaagatac attgatgagt ttggacaaac cacaactaga
      181 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      241 attataagct gcaataaaca agttttaatt aagcggggag gcggcccaaa gggagatccg
      301 actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg
      361 ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag
      421 aatccaggtg gcaacatagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac
      481 cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc
      541 gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca
      601 ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc
      661 acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa
      721 ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc
      781 gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat
      841 tttgtaatcc agaggttgat ttcgcgagat atctgcagaa ttcttatctc acggcgccgg
      901 ccatccaggc gctcagctct ctcagctcct tctcctgctc ggggaagtcc accagcaggg
      961 cctcgcactc ctgctgcagc atgtcggctc tgtacaggca gccctgcagt ctgccggcca
     1021 gggcctccag gggggcgggg ttcaggctgt cggtgaacac ctgggctctg gtgatgtggc
     1081 ccttctccac gtcgaagtgc agctccacgc cgccccaggt gaatctctcg tccagcaggt
     1141 ggctgaaggc gggggcctgg ccgaagttcc actcccagct gctctgtctg gcgaaggtct
     1201 cggcgaagtt gggcaggtcg ggggtcttgt tggggctgat gatctcggcc tccactctct
     1261 cgccgtagtg ggcgaagaag gcctcggtga tggcctcgca cacctgctcg tgggtgatgc
     1321 cgggcagcag ctcggtcagg ttggtcactc tgcttctcac gctggtgatg cccttggcgg
     1381 ctgccttctt cttgtcgggg ttcaggtagt tggccagtct gctcaggtcg gcgttcagca
     1441 gcagggtgcc gtggtggaag cctctgtcct tggtctctct gtaggcgctg ccgctcacct
     1501 ttctgtcgcc ctccacggtc ttcaccacca ggtcgtttct gccgctggcc tcggcgctca
     1561 cgcccagggc gttcagggcg ttcagcacga tgctggtgct gatggtcttg tcgtactcgg
     1621 gcttgccggc catgaaggtg aagcaggtgt tgcccaggtc gtggaacacg gcgccgccgc
     1681 cgctgcttct tctggccagt ctcacgttgt cctcctccat tcttctggtg ttgcactcct
     1741 tccaggggtt ctgggctctg ccgatcacca cggtgtcggc gtttctcacc aggaacagca
     1801 ctctctgggt ggcgggcatc tgtctgaaga tgcactcctc cacggccagg ttgaaccagg
     1861 ggtcgtagct gtcgctgatc agcagtctca gggtgctcat cttatcgtcg tcatccttgt
     1921 agtctggatc cttcttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     1981 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     2041 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     2101 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     2161 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     2221 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     2281 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     2341 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     2401 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     2461 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     2521 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     2581 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     2641 tgctagccat accatgatga tgatgatgat gagaaccccg caccatggtg gcggccgcaa
     2701 gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct
     2761 ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     2821 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     2881 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     2941 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     3001 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     3061 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3121 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3181 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3241 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3301 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     3361 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     3421 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     3481 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     3541 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     3601 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3661 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3721 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3781 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3841 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3901 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3961 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4021 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4081 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4141 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4201 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4261 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4321 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4381 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4441 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4501 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4561 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4621 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4681 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4741 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4801 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4861 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4921 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4981 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5041 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5101 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5161 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5221 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5281 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5341 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5401 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5461 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5521 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5581 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5641 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5701 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     5761 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     5821 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5881 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     5941 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     6001 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg
     6061 cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
     6121 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     6181 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     6241 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     6301 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     6361 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     6421 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     6481 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     6541 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     6601 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     6661 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     6721 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     6781 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     6841 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     6901 tcatcctgtc tcttgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca
     6961 aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag aggcagaggc
     7021 ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga
     7081 actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta
     7141 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct
     7201 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     7261 ctttccacac cctaactgac acacattcca cagaattaat tcgcgttaaa tttttgttaa
     7321 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7381 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7441 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7501 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7561 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7621 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7681 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg atacccccta
     7741 gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
     7801 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
     7861 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
     7921 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
     7981 caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg acactataga
     8041 atagggccct ctagatgcat gctcgagttg cgccttttcc aaagcagccc tgggtttgcg
     8101 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     8161 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     8221 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     8281 tgccggacgt gacaaacgga agccacacgt ctcactagta ccctcgcaga cggacagcgc
     8341 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     8401 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     8461 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     8521 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccgcca
     8581 ccatggtgtc taagggcgag gagctgatca aggagaatat gcacatgaaa ttgtatatgg
     8641 aggggacggt agataaccat cacttcaaat gtaccagtga gggagaaggc aagccctacg
     8701 agggcactca gaccatgcgg ataaaggttg tcgagggtgg tcctctgccg ttcgcctttg
     8761 acatactcgc tacatccttt ctgtacggtt ccaagacttt tattaaccac acgcaaggca
     8821 taccggattt ctttaaacag agttttcccg aaggtttcac atgggagcgc gtgactacgt
     8881 atgaggacgg aggagtgctc accgctacac aagacactag cttgcaagat ggttgtttga
     8941 tctataacgt caagattagg ggagtaaact ttaccagcaa tggcccagtc atgcagaaga
     9001 agacgttggg gtgggaagca ttcacggaga cattgtaccc ggcagacggc ggcctcgaag
     9061 gtaggaacga catggcactg aaacttgtgg gaggaagtca tttgattgcg aatgccaaga
     9121 ccacctatcg gagtaaaaag cccgcgaaaa atctgaagat gccaggtgtt tattatgtcg
     9181 attacaggtt ggagagaata aaagaggcta acaatgagac ctatgttgaa caacacgaag
     9241 ttgcggtagc ccggtactgc gatcttccaa gtaagttggg gcataaactt aattgatagg
     9301 cggccgcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9361 ttgaccctgg aaggtgcca
//
