LOCUS       sequence-495427-        9472 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  A CAGGS driven expression vector containing FastFUCCI to label cell 
            cycle state followed by an H2A-tdiRFP for nuclear labeling. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 9472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)
FEATURES             Location/Qualifiers
     source          1..9472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1745..1769
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1797..2447
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             2502..2774
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             2781..2834
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2841..3518
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             2841..3518
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             3549..3878
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             3894..3950
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6377..6401)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6493..6512)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6558..6613
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6612..6631)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6974..6990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6974..6990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6987..7009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6998..7014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7022..7028,7029..7046,7047..7052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7067..7088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7147..7342
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7193..7328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7348..7482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7385..7404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7439..7458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7550..7567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7721..8309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7801..7820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8480..9271,9272..9340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9103..9122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9341..9445)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggnsgcgc gccrggcggg gsggggsggg gsgrggggsg
      541 gggsggggsg aggcggagag gkgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga
     1741 tatcacaagt ttgtacaaaa aagcaggctc cttagattaa ttaagaattg ggatccatgg
     1801 tgtccgtgat caagcccgag atgaagatgc ggtactacat ggacggcagc gtgaacggcc
     1861 acgagttcac catcgagggc gagggcaccg gcagacctta cgagggccac caggaaatga
     1921 ccctgagagt caccatggcc gagggcggac ccatgccctt cgccttcgac ctggtgtccc
     1981 acgtgttctg ctacggccac cgggtgttca ccaagtaccc cgaggaaatc cccgactact
     2041 tcaagcaggc cttccccgag ggcctgagct gggagcggag cctggaattc gaggatggcg
     2101 gcagcgcctc tgtgtctgcc cacatcagcc tgcggggcaa caccttctac cacaagagca
     2161 agttcaccgg cgtgaacttc cccgccgacg gccccatcat gcagaaccag agcgtggact
     2221 gggagcccag caccgagaag atcaccgcca gcgacggcgt gctgaagggc gacgtgacca
     2281 tgtacctgaa gctggaaggc ggcggaaacc acaagtgcca gatgaaaacc acctacaagg
     2341 ccgccaaaga aatcctggaa atgcccggcg accactacat cggccaccgg ctggtgcgga
     2401 aaaccgaggg caacatcacc gagcaggtgg aggatgccgt ggcccactac agcatgctgc
     2461 ctagcctcgg cggctaccct agccattgga ggcccctgga accctctcct gccagacctg
     2521 ccctgagagc ccctgcctcc gccacctccg gcagcagaaa gagagccaga cctcctgccg
     2581 cccctggcag agatcaggcc aggccccctg cccgcagacg gctccggctg tccgtggatg
     2641 aggtgtccag ccctagcacc cccgaggccc ccgatatccc tgcctgtccc agccctggcc
     2701 agaaaatcaa gaagagcaca cctgccgccg gacagcctcc tcacctgacc agcgcccagg
     2761 accaggacac catcgtcgac gagggcaggg gcagcctgct gacctgcggc gacgtggagg
     2821 agaaccccgg ccccagatcc atggtgtccg tgatcaagcc cgagatgaag atcaagctgt
     2881 gcatgcgggg aaccgtgaac ggccacaact tcgtgatcga gggcgagggc aagggaaacc
     2941 cctacgaggg cacccagatc ctggacctga acgtgaccga aggcgcccct ctgcccttcg
     3001 cctacgacat cctgaccacc gtgttccagt acggcaaccg ggccttcacc aagtaccccg
     3061 ccgacatcca ggactacttc aagcagacct tccccgaggg ctaccactgg gagcggagca
     3121 tgacctacga ggaccagggc atctgcaccg ccacctccaa catcagcatg aggggcgact
     3181 gctttttcta cgacatcaga ttcgacggca ccaacttccc ccccaacggc cccgtgatgc
     3241 agaaaaagac cctgaagtgg gagcccagca ccgagaagat gtacgtggag gacggcgtgc
     3301 tgaagggcga cgtgaacatg cggctgctgc tggaaggcgg cggacactac agatgcgact
     3361 tcaagaccac ctacaaggcc aagaaagaag tgcggctgcc cgacgcccac aagatcgacc
     3421 accggatcga gatcctgaag cacgacaagg actacaacaa agtgaagctg tacgagaacg
     3481 ccgtggccag gtacagcatg ctgcccagcc aggccaaggg ctaccccagc cactggcggc
     3541 ctctggaaat gaaccccagc atgaagcaga agcaggaaga gatcaaagag aacatcaaga
     3601 acagcagcgt gcccaggcgg accctgaaga tgatccagcc cagcgccagc ggatctctgg
     3661 tgggccggga gaatgagctg tctgccggcc tgagcaagcg gaagcaccgg aacgaccacc
     3721 tgaccagcac cacctctagc ccaggcgtga tcgtgcccga gagcagcgag aacaagaacc
     3781 tgggcggcgt gacccaagaa agcttcgacc tgatgatcaa agaaaacccc agcagccagt
     3841 actggaaaga ggtggccgag aagcggcgga aggccctggg atctggcagc ggcgccacga
     3901 acttctctct gttaaagcaa gcaggcgacg tggaagaaaa ccccggtccc ggtaccatgg
     3961 caggtggaaa agcaggtaaa gacagtggaa aagccaagac gaaagccgtc tccaggtctc
     4021 agagagctgg tctgcagttc cctgtaggtc gtatccacag gcacctgaag acccgaacca
     4081 ccagccatgg ccgtgtcgga gctacagctg cggtttacag tgctgctatc ctggagtatc
     4141 tcaccgctga ggtgctggag ttggctggaa acgcttctaa ggatctgaag gtgaagcgaa
     4201 tcactcccag acatttacag ctggccatcc gcggagacga ggagctggac tcgcttatca
     4261 aggccaccat tgctggagga ggagtcattc ctcacatcca caagtctctg attggtaaga
     4321 agggccagca gaaaaccgca gatccaccgg tcgacaccat ggccgaaggg agcgtggcaa
     4381 gacagcccga tctgctgaca tgcgacgacg aaccaatcca cattccagga gctatccagc
     4441 ctcatggact gctgctggct ctggccgctg acatgactat cgtggcaggc tctgataacc
     4501 tgccagagct gaccgggctg gctatcggag cactgattgg ccggtccgca gccgacgtgt
     4561 tcgattctga gactcacaat agactgacca tcgccctggc tgaacctggg gctgcagtgg
     4621 gagccccaat taccgtcggc ttcacaatgc gaaaggacgc tggctttatc gggtcctggc
     4681 accgccatga tcagctgatt tttctggagc tggaaccacc tcagagagac gtggcagagc
     4741 cacaggcttt ctttcggaga acaaacagcg ccatcaggcg cctgcaggcc gctgagactc
     4801 tggaatccgc ttgcgcagcc gctgcacagg aagtgaggaa gatcaccggg ttcgatcgcg
     4861 tcatgatcta cagattcgcc tcagacttta gcggagaagt gatcgcagaa gatcgctgtg
     4921 ccgaggtcga aagtaaactg ggcctgcatt acccagcctc aaccgtgcct gcacaggcac
     4981 gacggctgta tacaatcaac cccgtgcgga tcattcccga cattaattac agaccagtgc
     5041 ccgtcacacc tgacctgaac ccagtgactg ggaggcccat tgatctgagc ttcgccattc
     5101 tgcgcagcgt gtcccctgtc cacctggagt ttatgaggaa tatcggaatg catggcacca
     5161 tgtctatcag tattctgaga ggcgaaaggc tgtgggggct gatcgtgtgc caccatcgga
     5221 caccttacta tgtggacctg gacggcaggc aggcttgtga actggtggct caggtgctgg
     5281 cttggcagat tggcgtgatg aaggaggggc atggcaccgg cagcaccggc agcggcagca
     5341 gcggccgctc cggcaccgcc tcctccgagg acaacaacat ggccgccgaa gggagcgtgg
     5401 caagacagcc cgatctgctg acatgcgacg acgaaccaat ccacattcca ggagctatcc
     5461 agcctcatgg actgctgctg gctctggccg ctgacatgac tatcgtggca ggctctgata
     5521 acctgccaga gctgaccggg ctggctatcg gagcactgat tggccggtcc gcagccgacg
     5581 tgttcgattc tgagactcac aatagactga ccatcgccct ggctgaacct ggggctgcag
     5641 tgggagcccc aattaccgtc ggcttcacaa tgcgaaagga cgctggcttt atcgggtcct
     5701 ggcaccgcca tgatcagctg atttttctgg agctggaacc acctcagaga gacgtggcag
     5761 agccacaggc tttctttcgg agaacaaaca gcgccatcag gcgcctgcag gccgctgaga
     5821 ctctggaatc cgcttgcgca gccgctgcac aggaagtgag gaagatcacc gggttcgatc
     5881 gcgtcatgat ctacagattc gcctcagact ttagcggaga agtgatcgca gaagatcgct
     5941 gtgccgaggt cgaaagtaaa ctgggcctgc attacccagc ctcaaccgtg cctgcacagg
     6001 cacgacggct gtatacaatc aaccccgtgc ggatcattcc cgacattaat tacagaccag
     6061 tgcccgtcac acctgacctg aacccagtga ctgggaggcc cattgatctg agcttcgcca
     6121 ttctgcgcag cgtgtcccct gtccacctgg agtttatgag gaatatcgga atgcatggca
     6181 ccatgtctat cagtattctg agaggcgaaa ggctgtgggg gctgatcgtg tgccaccatc
     6241 ggacacctta ctatgtggac ctggacggca ggcaggcttg tgaactggtg gctcaggtgc
     6301 tggcttggca gattggcgtg atgaaggagg aataactcga gtaatgataa actagtgttt
     6361 aaactacgta actaggaccc agctttcttg tacaaagtgg tgatatcgcg gccgcactcc
     6421 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     6481 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     6541 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     6601 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     6661 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     6721 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     6781 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     6841 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     6901 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     6961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7141 ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7261 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7321 tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg
     7501 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7561 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7621 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7681 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7741 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7801 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7861 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7921 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7981 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8041 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8101 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8161 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8221 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8281 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8341 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8401 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8461 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8521 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8581 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8641 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8701 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8761 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8821 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8881 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8941 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9001 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9061 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9121 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9181 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9241 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9301 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9361 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9421 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gg
//
