LOCUS       sequence-495438-       12741 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  LuxCDABE reporter vector for Ralstonia pseudosolanacearum 
            filamentous..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12741)
  TITLE     A filamentous phage-based versatile toolkit for molecular studies in
            Ralstonia pseudosolanacearum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSCqlux
FEATURES             Location/Qualifiers
     source          1..12741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(183..1166)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(1185..2267)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(2316..3239)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(3252..4694)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             complement(5302..6117)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5302..6117)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6025..6044
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6109..6128
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    7016..7034
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(join(11633..12741,1..4))
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
ORIGIN
        1 tcatacctgt tttactcctc aagataatat tagaaagtat ggcagcactg ctgtcatact
       61 cttttatacc cttcatcttt caagctgctg ctttgttggc tgctttcact caccccagtc
      121 acatagttat ctatgctcct ggggattcgt tcacttgccg cctcgctgca actcgaaatc
      181 tattaggtat attccatgtg gtacttctta atattatcat caacaatatt gattacattt
      241 ttttggctca tcaaatcatt cattggttca aaggacagca atacactttt cgcaccacac
      301 ttttcaattg ccaacttagc cgcagttata cactccgtat aatttccgac agcgttttct
      361 gcaattattt cttcaagttt attttcgaaa ttttcattag ggtgcatttc aagaacataa
      421 tcactaataa atgcacgcgt ctcttgttta gctttattac tatcttcgtt atagttaact
      481 aatatcatta actgatggtc tatctctgat aggtcaacgt catatttatc cgcaacggct
      541 ttatatcttt cagcatattc atatctaaca tcattagaat catcccactt aaagatgaga
      601 ggaatacctt ttttggccgc ccactcaaca atatgatgac tggttgctgt tacatatttc
      661 cgaggtccgc ctggcgtata agcatgggga tttacagata ttttagggaa gctataaaaa
      721 tcgttatctg gattacaata gcctgttgtt aaagcatcgt taatgatttc ataacactct
      781 tcaaatagtt gctgttgata ttcaaccggg cgattaaaaa aatgcatttc atcttttttt
      841 tcgcaatcac taaaccctaa aataaatctc ccttcactta actgatccaa taagcaagct
      901 tcctccgcta tgcggacagg atgatgagtt gtaatgatgt gatttaatga accaatttta
      961 attttctctg ttaaaccgag cagaaaacca gaaacagtca gaggagcgcc gacaacacca
     1021 ttatctgaaa aatgattttc atacactaaa atctgttcaa aattcaactt atcaacatac
     1081 tccgttattt cctgcatgcg aactatactt tgttcttgaa cagttgttga attgatgaag
     1141 ttaaggaaga acaatccaaa tttcatttct ttctccttag ccttctaata taatagcgaa
     1201 cgttgttttt ctttaagaaa tggcatgaca tcagactgga agagcttcat ggaagcaata
     1261 atttcgtcta ctgttccatt agcttcaaat ccacaacaaa tatttgatat tcctgtagca
     1321 tcaatgtctt tttgaattat gtcaatacat tcctgcggcg ttcccacggg attgatttcg
     1381 taactgtaat caatacggcg attagtatct ttatgtcctt ttaatacaaa gtcacgccac
     1441 tgccctttat tgaaatcata acctcttgtt tggtctgaat catcaaaaat agtcgtagca
     1501 ttcacataag aatcatacca atgccccaga aatttccggc aaatctcttt cgctttaatt
     1561 gagtcatgat ctacagatgt tatatatgat aagcaatggt cgatattatg aatatcgtgc
     1621 ccatattctt gagccacttc attataaagc tcaagttgtg ctttcttttc gttagtattt
     1681 ataatccaac ttaatatcat cggtaggcca aattgagcag cccactcagt cgtcgaagct
     1741 gattcagcca ccacataaac cggtgcgcca cctctgctat acgccgcggg gtttactttt
     1801 accttatgga acttgatatg ttcattatca gcttccatat atccctctgt catgccattc
     1861 tttatcagcc cgtaccagca ttccgctaag gcgcgactgt tattcatatc tgtgccgaat
     1921 acgcgaaagt ccttgttgta aagccctcgg caaataccaa accgaaatcg tccttttgac
     1981 atttgatcca ataaattcac atcttcaagt tggcgtactg gatgggctgt gggaagaaca
     2041 atagcggcag ttcctacatt caatttttta gtcgcgccaa gtaaatatgc agcagcgaca
     2101 taagggttac caagcaaacc aaactccgtg aaatgatgct ccagtaacca tacggtatca
     2161 aaaccacact cctcagagat gcgacctaat ttaaccaaac gtttcattac ctctgtttga
     2221 gaaaattggg gaggttggta tgtaagcaaa aagtttccaa atttcataga gagtccttat
     2281 attgctattt gagtgataga atatctcaat agattttaag acagagaaat tgcttgattt
     2341 tcaatctcaa ttctcattcg gcgttcattg actgtcgcaa tagttaaatg ttcaaatgac
     2401 ggttcagtaa tatcaacatc aatatccaga tgatcattat ccatcgcgat agcggctttc
     2461 gtaaccgatt gataaaaatt gcgcaggacc actaaatttt cactcaagtc atgcgaactt
     2521 cctaacaaag aatatatctt gcatcgatta ctacgaatat ttgataacaa tgtgataact
     2581 tcatcttgct tgacccaatt atcgttattt gcagtaaaag caataaacgg tatatcaaga
     2641 tacatcatgt tattaattgt agaagctaaa tcttcccaac caaaatcaag acaatctctc
     2701 gcaaagactt cagcacccaa tttatggcct tcaaaatcta gattattcgg caattcatta
     2761 atgggtagac tgagataatc aaaccctaaa gctctttcaa gagaatatct taagttaaca
     2821 acaccgactg cggtgattaa aaacgaagca ttgatttcag ataggcttgc ataagctatc
     2881 cgcgcagata agcttgaagc caacataccg aagttattta tttttcgtgt agttaaccaa
     2941 tcaaccactg ctaacaagct ctgctttcct atagacattg taaattcatc aattgtccct
     3001 gaactcaatc caacgtggtg aagcgaatca tagcggatca catgaaatcc attccgcgat
     3061 aaatattccg ccagaccagc aaaatgatcc atcctgcggg caaaaccaga cgcaataata
     3121 atggcattct ttctctttgg gctgttttct tctggcagcg tttcccaaac atgaattttt
     3181 ttatttcctt caacacaaat aacgtggtcg atggttttat attttgattc attttccata
     3241 ctttttacct attatgggac aaatacaagg aacttatctt cttccaggaa tcgagtctgt
     3301 tctatttcaa ccgcaacatc cttagccgta tagttagatg gcctttcatg agaaatatat
     3361 gtcactaatc gttgcaacgg tcgcattccg tcatgagatc caccaactcg aaatatgtta
     3421 ttcattcctg cttctacaat cctttccgca ccttttaatg ctaacgcatc tcgatattta
     3481 aatgatgact cccaaggaaa aatagatatg gtttgcgtct tatttttttg aacataaggc
     3541 aatatttgct caatattatc gacgtgatga aggtacacac atctgccaag tggttgatta
     3601 aattccacac ctgcatttga ctcaataatc atccaacgtt gatgaatatc cacctctact
     3661 tttaatccag caaacaagct ttctttttga actaaagaat aggccgcctt ttcatcaaaa
     3721 tcttttttgg cattcggtaa tatatgcgca tatagattaa gtttttctat caacgctaac
     3781 ttaaattcct cataatgatt tcccatgtaa tatatgtttt gggcagaaaa acaagctcgc
     3841 tgatcgtaaa aacaaacatc atgagccgca cctgtcgctg cggacgtcaa atcaacagga
     3901 ttatcgataa tgcaaagact ctttttagaa ccaaatttaa tcacatcagc ataagatggc
     3961 gcatgctcta ccgcccaatt aatcgcatct ggccctcccc aagcgacaat aacatccgca
     4021 tgtcgcataa tttcttttgc gagtgatgta tcaccttggt ggggccaata tataacagat
     4081 aaagagcgcg ttatcggatg attagggtct acatcaataa aacttaacgc taatgcatta
     4141 gcggtaaaag gatcggttga cgatgttttt ataatacact gattcttagt taaaattgcg
     4201 cgtaatatag acatgatccc agataatgga acattacctg ccaacagatg tacagattta
     4261 cctttcggaa aagcccgaac ataactttca tcctgaggta gccattcatc catgatatgg
     4321 cgagaaccaa gttcattttc tacaacatca taaaggccgc ctttagaaca taaaatcata
     4381 gatatccaat tggcctctag cttagccatt tcttctgaat atcccatata tttttttaag
     4441 tcacgaatgt atgtcctgcg tcttgagtat tcttcatttt tccatctttg ccctaccgta
     4501 tagagaaaat tgacaatgtt atgcaaccgt aattcgttat ttccattaca atcaataatg
     4561 ttttttacat gagagtcatt caatattggc aggtaaacac tattatcacc aaaattaatg
     4621 gattgcacta aatcatcact ttcgggaaag atttcaacct ggccgttaat aatgaatgaa
     4681 atttttttag tcatggtacc catgcgaaac gatcctcatc ctgtctcttg atcagatctt
     4741 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     4801 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
     4861 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
     4921 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt
     4981 ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc
     5041 ttgcggcaga attctggctg aagcttgcat gcctgcaggt cgactctaga ggatccccgc
     5101 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg
     5161 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag
     5221 ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
     5281 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     5341 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     5401 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     5461 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     5521 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt
     5581 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     5641 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     5701 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     5761 attttcacct gaatcaggat attcttctaa tacctggaat gctgtttttc cggggatcgc
     5821 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     5881 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     5941 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     6001 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     6061 catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac
     6121 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     6181 atcttgtgca atgtaacatc agagattttg agacaccctg tgaatgcgca aaccaaccct
     6241 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc
     6301 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
     6361 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
     6421 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
     6481 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc
     6541 tatgtcgggt gcggagaaag aggtaatgaa atggcagatc cctggcttgt tgtccacaac
     6601 cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     6661 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     6721 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     6781 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     6841 aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttcg gatctatgtc
     6901 gggtgcggag aaagaggtaa tgaaatggca tccggatctg catcgcagga tgctgctggc
     6961 taccctgtgg aacacctaca tctgtattaa cgaagcaatt catcgatgat ggttgagatg
     7021 tgtataagag acagacaata atcgttgagc tgtcgaatgg ctcaacggcg cggctttggc
     7081 gggagagggc gcaacaaaat ttgttgcgct cgcgaccagt accaggagga gggcggctgt
     7141 cgcgttactg gctgccatcc tcgtcgaaaa aacttcccag agagagtgct ggccagcacg
     7201 ttcggcgcgg tccatgtcgg ccgccatgat gatcggcagc gggttctcca tctcgagcag
     7261 ctgcgccagg cggaggcaca cttcgttgtc cgccgtgcgg cggccggtgc ggtagtggct
     7321 gaccgcgctt tgcaccacac ctagcatctc tgccagcttg ctgtcgttct tcaggccgtg
     7381 gcgttcgatc gcctgatcta agtatttctc aattctcatc taagcacctc tttgcttgac
     7441 ggcatgaagg atgtaattaa tattatgtcc gtgatgaatt acgcccgtga ttctggcgta
     7501 aatatagccg gttaacccgg gtaaacgatg cagcagagag atcataggca gctggacctg
     7561 attggcgacg tgcttcaccc gcacggtgcg cagctggcca tcgtgatggc tgccgttctg
     7621 cgcggcgcct gctgcctgca atcgcgtcct gcgatgcatc gcattgacga cctcctgaag
     7681 aactcgcgcc aggccgatct ggcgaactgg tatcgctgcg agggcgcacg ctgatgcgcg
     7741 tccgtcccac cccctctcgt catcttgatc ccacaggcat tgcgtatgga gttcatgggc
     7801 cggcgctcgc acgcgagaac ggcatggcgg ccgaccgggc cgagcggagc gaggctgggc
     7861 ggccgacagg ccgttccccg ggtccggtaa aacccgggga aagtccagaa cgcaaggcag
     7921 gtgcgatcgt ggactggttc aagttcacgt tcctgcccga cggcagcatc agcgacgccc
     7981 tggagcagct gcgcaggtac ttccagctgg tgttctcggt gccggtgacg atgaagcctg
     8041 cgggcaaagg gttccgccgg tatgagttca gctacgacct gctggcgttc atcaacggcg
     8101 agacgatgaa gctcgggatc gttgcctgcg gcggcgagca cgttggcggc acgatcctgg
     8161 tggactggcc ggggcagggc tgcaccgcga ttggcgactg gcaagccgtc tacgcgatgg
     8221 tgcaggacct cgacgcgcgg atcacgcggt gcgacctggc gatggacttc tgccagggcg
     8281 aggtgagcat cgcgcagatg gaagagctgt actacgccgg cgacttcaac gcgggcgggc
     8341 gtatcccgac gtatcgcaag atcgagagcg gcgtggcggg cagcaagggc tgtcgcggta
     8401 cgacgttcga gattggccgt cgggcgaacg gcaagatgct gcgggcttac gagaagggcc
     8461 gccagctcgg caatcaggat agcgagtggg tgcgtctcga gatcgagttc ggcgccaagg
     8521 atcgcgtgat tccgcacgaa atccttatca agcgcgacca gtacttcgct ggtgcgtaca
     8581 aggccctgga ggccttcatg gctgcggatc cgcagcgcgt tcctactgac caggtcaagg
     8641 ccttggagct tcaggacgaa accatccgcg aacgcaagct caagcacatc cagacgcagt
     8701 tcggccctac ggtcgattac gagctgcgct gcacagagga agacatcgct gcgctggttg
     8761 tcgcgatacg ccgtcagggc gtaccggctc agctgcacaa atccgccctg gcgaggcacg
     8821 tgtacggcac gcacgatcca gtgccgaaac ctgaggagtg aaaccatgga aatgattgca
     8881 cgcgtgacca tccggggcgc caagacgtgg gttggcaaca tggatggcaa gcagctggac
     8941 accgggaaga tctacacgga tgtggagctg cgtggcgaag actcgaaggg cacctgcacg
     9001 caggagctga agtgcgagag ctctgcggtt gtgaaggcga tcatccacaa cccgttcccg
     9061 ttcatcgcgg aagtctcgat ggtcgagacg agcaacggca aggataaggg cggtcagaag
     9121 gtcgttacga gcatcaagcc gctgcagcgt gctgaggctg agcccaagaa ggcgtgatgt
     9181 tccctggctc tgggagttga acggcccgga gcttttctca acctccatct caaggagaaa
     9241 accgcgcacg gtgctttgcc gattggctgc tgacggcgcg ttggtgggcg cacggtgctt
     9301 tgccgatgtg ggcatggcca cggatgccta ttacagcgcc gtcgctccgt cgcagacacc
     9361 tggggcagtc acctatctgt ctgagttcgt caagaccacg ggcgggtggg tgctgcggcg
     9421 gtatcaggtg ggcagtgatg ggagcgtcgg cacgttgacc gatgcgtcgt tgccgtcgct
     9481 gtcgtttcct gcatgtgacc cgcaagagag cttcaaggat ggcatgacga tggggtgggg
     9541 cgtcgttgcc gcgatggtgg cggcctgggc gctggtggtg atgaaaaagg ggctgtgatg
     9601 ctcgcgatcg atttctggct ctgggccgga ttcctgctgc cggttctgcc tagcgtgacg
     9661 taacggatta ggttcctcgc gcaacgcaac cgatcacgct gatcacggcc acgcctggtg
     9721 gcgggaagac agcgttggcg gtccagatga tgaaggcagc cgtcgaccag gggcgtccgc
     9781 tcttcgtcat gggcatcccg gagctgaagc tgccgtatat cccgacgccg gcggtttcgg
     9841 actggacgga gctgcgcgaa gaccctgaaa acccagggat gatgctgccg tacttcacct
     9901 tcccgccgaa ctcgctgatc gtgttggatg aggcgcagcg ggtgtttcgc gtccgcacgg
     9961 ccgggtccaa ggtgcctgac cacgtggcgg cgtttgagac ggtgcggcat accggcgtga
    10021 cgtttgttct gatcacgcag aacccgacgt tcttggacag tcatatccgc aagctggtgg
    10081 ggcagcatgt ccacctgcgg gacgctggct tgctcggacg ctggtactac gaatggccgg
    10141 agtgcgcgaa cccggagacg ttcaacaccg cgccgatcaa gaagaagtgg agcctgccga
    10201 agtccagttt cggcctgtac aagtcctcca gtctgcacat caagcgcaag tacaccgtac
    10261 cgccggtgct gatgctgttc atcgcctgcg tgttgatcgc ggcgttcttg gcgtaccggg
    10321 tctattaccg taccggccag ttgacgacgg cgactccggc taagcctgtt gcggccgagc
    10381 agggcagggg cggtgtggct tcagctctag cggtagcggt caaggcgggt ccgtccgccg
    10441 cggcgacgga cggggcggag atcctcgcgg cgttcgtccc tgctgtctct ggccggcctg
    10501 agacggcgcc ggcctatgcg cagttgcgcc agattcgctc gatgccgacg gtgatcggcg
    10561 gtgcctgcac gtcgaccagg tgcacgtgct acacggctca aggcacggat gccggcctcg
    10621 atgacatgca gtgtcgggag tggatcagga ggccgccgtt tgacccctac cgcgagccac
    10681 aagcggcgca ggagccaatt tcggcgcagc tgacccctgc gaccgcccag tcagaaaaaa
    10741 cggctccaaa gcccgctgag ggcgtctagc gccggggctg ctcgcgaaat ttccgttacg
    10801 aactaaattg taacgataat tgaattgccg ttaccagtaa cgtaaaatgg aatcatcaaa
    10861 tcgggagccg aatatgcgtg acgtaacgga taatgtgacg gtcgatctgc cggggatgga
    10921 gcaaaggcgt ggccgcggtc gtccgcgtaa ggcgcacgct atgaccaacg cggagcgtca
    10981 agccgcatat cgagctcgcc gtaaggctca gcagcctgtt gaggctaaat ccgttactaa
    11041 gcgtatgacc gtaacggaaa tgctttccga cgtggatgct tacgatgaat gccgcctgga
    11101 ggtcgagaag tttcgcgctg atctggtgag catgcgtcgc caggtggagc tggcggaggc
    11161 tgaacgcaat aaggcgtttg ctgagaatag gcggctgagg gaagagatca gcgatgccgc
    11221 caaatccgtt acgctgagta acggaaatct tgcggagatt gaggcgttgc gccggcagct
    11281 tatgacgtgc gaggacgggc gtcaggaagc gttgcgttat gttggcgtct tggaagagaa
    11341 ggttgcgatg tttgagtcgc agcaaaaatc cgttacgccg agtaacggaa atcccgtttc
    11401 gttcgacgca atgctggact tgctcgcgtt ggctgcgaag gcgaatacgt tcgaacagcg
    11461 ccagaaggtg cgggagtccg agttgtggcg cgcttcgttt gtccgttctg tggccgtgag
    11521 tgaggcgcag atgcaagcgg ctggcgaggc aatttatggt gatcgcaaag tcgttacgcg
    11581 caaggcgtaa cgcgaaacgt ctaaaatgat ccggttattg gagggagcgc gttcaactat
    11641 caaacgcttc ggttaagctt aaagcacacc ctttctgcgt cctcgtattg acgcgacgta
    11701 aaatttcaac gagcacgccg ggatacttac catattctct gctaattatc ccgacatcat
    11761 cggtaacaat aaatgctgga taactggttg ctgacgcatc catataactc atcaaccccg
    11821 gcgttccatc aggtacaggt ttcaacgttt caggatcaag cgctcgcgca tatacccacg
    11881 gcggaacatg tttacgctgc atttcatcct caaagaaaca agtgttgagt tcaacttgat
    11941 taaatatatc tcggatctga ctaatatcac tgagattgaa agtatcaaat aaaagatgat
    12001 tgaaatcatc acgtttcaga gattcttttt cgtaactttt ccagccgcct ccggttatga
    12061 tataaaggct tttatctcca gaaaatgaga tttttttatc tttcatataa tggcagagta
    12121 aataaataaa gtatggcgaa ccaataagac aaagatcttt cccttgattt tttattcgtt
    12181 caagactatt caatgtttta acaaaatcta ttcgttcttc tgttacggta aatgtcgtag
    12241 gatataacaa ttccaccaaa ctcataacat atttaaacca aatattatga gcattaaatc
    12301 tatctggtcc caaattgact aattctattt gatgatcaaa ccaactacca acatatttca
    12361 tgccataact cacagagcct aagagtctct caatacttaa tctgtcacgc gccacctgac
    12421 tttttaaacc attcgtgccg ctactggtaa accaactttc aatctcgttt tcctgagaag
    12481 ttaataagcg agtaaactta aaaaccgatg ttgggaatac aggtatgtca tcaatttccg
    12541 taatattgtc atctactttg tgtgcctgac agtagtgacg atattctcga caatgtttat
    12601 aatgattacg aaatgcatca agcacaagtt tctttctgat tttttcctgc tcgtcgtaag
    12661 accacactaa tggatcgctc gaaaaaatca aatcatcaat ttctgagctt gctgtaattt
    12721 cttgtttatc aacatatgaa g
//
