LOCUS       sequence-495454-       10599 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  A Tol2 expression vector containing the fli promoter driving FUCCI 
            to label cell cycle in all blood vessels followed by an 
            IRES-mTagBFP2. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 10599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)
FEATURES             Location/Qualifiers
     source          1..10599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3160..3184
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3215..3280
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(3293..3295,3296..3298,3299..3811)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(3338..3359)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3599..3618)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3949..3969
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4349..4405
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4406..5113
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4551..4569)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4819..4838
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(5744..5768)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5769..6343,6344..6346,6347..6355)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5936..5953)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6163..6182
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6359..6424
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6443..6463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6467..6487
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6491..6511
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6533..7243
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     polyA_signal    7300..7434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7496..7516)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7524..7540)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7556..7690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8129..8316,8317..8328)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8362..8378)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8362..8378)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8415..8435)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8415..8433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8454..8470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8467..8489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8478..8494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8502..8508,8509..8526,8527..8532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8547..8568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8685..8702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8856..9444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8936..8955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9615..10406,10407..10475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10238..10257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10476..10580)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggat ctcatcttga cccataaaca
     1141 tacactaaaa cccaacagtc aactttatca aatgaaatga gtgtaattaa ctcaaaattt
     1201 actgaatgtt gattctactc attcgaaaag agttttaaac tcagtgttga aggtagtgag
     1261 ttcattaaat acctcattac ttcaacttaa ctggagtaag ttcatggtac tcacatagat
     1321 tagtttagct caaatggttt gtagcagtcg gttccctcaa gcagtttgag ttgccttaac
     1381 tttttgggtt ttacagtact cagttggttt gagttcactt cacttattgg gttcaacatg
     1441 tgctcaaatt gcatcgttta atcaaataga ttaagttcac agtactcatt aggattagtt
     1501 tttgaactta aatggtttgt tgcagtcggt ttcctcaaac ggtttgagtt tccataactt
     1561 tatgggtttt tcagtgtagt aaacatatgc atccacagat tataatagta acctgcatgt
     1621 tttttccttc acaatttgac acagatacct cacatgattg acttttaact gtacacttta
     1681 gtcgacaatg agaagggtgt ctcaatctat gggtatttcc tgttccacac aatctggttt
     1741 cctgtcaggt ttccttttcc tcctgaaaca aaagacagga atgccctctg tataaaatag
     1801 cacagcgctt ttaatgggtc tctgtacatc ccgacctcat gacgcatctt ctgcgggaca
     1861 gccaatcacg atcaagttgg cttatgaaaa cagttttttg tccccctgtt aagttaaaag
     1921 cacggtagca tacatgcatt attcatcatg cagcatattc agcagtacaa agatgagctc
     1981 ttgacacacc tcagcgtaaa atctctattc cagcgtaatc ctgcggttct ggatggttta
     2041 agcgagcctt gcagacacgg ccaatttata gccatgttga aaatagcagc tttgtgagca
     2101 tttcgacctc aatgtaccaa gaatgcgtgc tcggtaactg aaaaagaggg ggtggagatt
     2161 ttggatttag agatggaatc acatgcacgc tgtatatgct atataaaaat agcaggcatt
     2221 taatgcatgc aaaatatgtt tcgacactaa acactacaag tcggaaatat atctattttt
     2281 ctttttttaa cgaaatgaga catttaatgc acagtaaatg tagtctaaca cgcacaatat
     2341 tcctattatt gtccatgaaa aatgtgaggt catatgctac tgatttaaag aaatgttgaa
     2401 cagcacagca cacaagaggt atcagcctct ctatatggtc catacagcac caggctcgct
     2461 gcgttcatgt gcattcagag gcgcgcacac gtgtggagca gacagactcg acgcagcggg
     2521 ctttgctttt tattcatgga agcccatttt agaagaagct cgggcagact cgtcacattc
     2581 ttcacttcac cataggtaga tcaaatcaaa gttattggat ataacggaac aatattcatc
     2641 atattatgag attattatta gaccgagtgc attagacata tttgtattcg atcgttgaaa
     2701 aagtatatat tttattttag ccttaaacta tggttagcct atattaatca gcataatctg
     2761 ttatttcaga cgttgtatta atgattattt ctagcacagc tttgatgcat caatgcaatg
     2821 tttctacaaa cccgccttgt ttaatgtcgg aaaaaaagcg caacaaatgt aaagagttgg
     2881 caggcattct gtaaatattt ccaggcaaaa acatgcacaa aggaaagaaa caccattgtt
     2941 ttggtgtctt atcttcgctg gacaaatatc ctgctttggg tgccgtaatg aaggcagggc
     3001 cagtgacaca cacacataca cacacacaca tacacgcata cacggcttcc ttctcaagcg
     3061 cagtgacttc acttcccaaa tttagaggaa aaaaaaccat ccggcgaaac tgtctctgtc
     3121 tctccgccac atatcggggg ctggaattaa ttcagacgcg caagtttgta caaaaaagca
     3181 ggctactgtc aagcttcagt caggatccgg tatggactac aaagaccatg acggtgatta
     3241 taaagatcat gacatcgact acaaggatga cgatgacaag ctcagatcca ccatggtgag
     3301 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3361 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3421 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3481 caccctgacc tggggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     3541 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3601 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3661 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3721 gtacaacgcc atcagcgaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     3781 ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3841 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3901 cacccagtcc aagctgtcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     3961 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaag gatatccatc
     4021 acactggcgg ccgctcgaga tgagttccat cagaagacca aagaatgcag aaaacccgtc
     4081 tgaaaacatc aagaaattcc tggtagcgcc aacatctgga gcaggaatgg ggaggaggac
     4141 gcttcaggtg cttcagccct ctgctgtaaa caaaaacctt ggtcgtataa ttgagaatgg
     4201 taaagcagtg gccaaaagga agatgtggag cgccgagcaa gttaagggtt caaagagagt
     4261 caaagcagag gtggctgtca aatccacaaa tgcagaaaat gaaaaccagc ctgagggggt
     4321 cacacaagag gcctatgagg gatctggagc cacgaacttc tctctgttaa agcaagcagg
     4381 agacgtggaa gaaaaccccg gtcctatggt gagcaagggc gaggaggata acatggccat
     4441 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     4501 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     4561 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     4621 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     4681 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     4741 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     4801 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     4861 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     4921 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     4981 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     5041 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     5101 cgagctgtac aagggatatc catcacactg gcggccgctc gagatggctc aagctcgtgt
     5161 tactgattat tttgcacaga gtaaaaaagc tggtgtttct cgatctttac ggtctaaggg
     5221 acaaaaggtg tccggggatg tagtagaatc agcggtgatc aataaaccga ggagttcgag
     5281 cagagcgtcc ggttcttcac gcaaagcgac gcacagtgag atcaccacag cagagccgca
     5341 gaaacaaact cagctggagt ttctcaaagt catcgacgag gctttatcga cggagactgc
     5401 agataccgtc gcggattcgc gtgatgtgaa gattgagggc ttaacggcga gtcccaggac
     5461 ccctaagcgg agttcaccgg agttcgacgt gtgttcggtg ttgtttccgt ccacagccga
     5521 gctgcatagc agcgccaaga agcggcagag actcaacgcg ggccacaact gcaggagctc
     5581 accggaggag cgcgctggac agaaaactgc gaggaaaaaa ctctatctac tggcaagcga
     5641 cgacaaagta aagaccactg agcccttggc cagcagcagc cctcaggcac ctcaacaaac
     5701 tgcccgcaaa gagtaaaccg gtacctgagt catcgatcag actacccagc tttcttgtac
     5761 aaagtggccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5821 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5881 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5941 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     6001 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     6061 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     6121 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     6181 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     6241 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6301 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgga
     6361 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
     6421 caaaggatcc accggtgaat tcccgaagaa gaaaagaaaa gtaggaccca agaagaaacg
     6481 caaagtcgga ccgaagaaga aacgaaaagt cagatctggc ggtgctggtg gtatggtgtc
     6541 taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     6601 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     6661 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     6721 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     6781 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     6841 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     6901 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     6961 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     7021 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag
     7081 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     7141 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     7201 cagatactgc gacctcccta gcaaactggg gcacaagctc aatgctagcg gctgatatct
     7261 ataatgaggc gcgccgtcga cacgcgttaa tgatacgtag atccagacat gataagatac
     7321 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7381 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     7441 atctagacat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttccaact
     7501 ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc
     7561 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     7621 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     7681 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     7741 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     7801 agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     7861 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     7921 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     7981 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     8041 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     8101 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     8161 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     8221 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     8281 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     8341 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     8401 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     8461 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8521 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8581 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8761 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9481 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     9541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10561 aaacaaatag gggttccgcg cacatttccc cgaaaagtg
//
