LOCUS       sequence-495461-        2733 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2733)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30001
FEATURES             Location/Qualifiers
     source          1..2733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3..5,6..8,9..467)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(48..69)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(309..328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(567..584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(738..1326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(818..837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1497..2288)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2510..2614)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2682..2700
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
       61 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      121 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
      181 ccctcgtgac caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
      241 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      301 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      361 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      421 acaagctgga gtacaactac aacagccaca acgtctatat catggccggt aacgcggcta
      481 ttagatcaat gtgagaccac gagtgattaa tgaatcggcc aacgcgcggg gagaggcggt
      541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat
     1501 ttgccgacta ccttggtgat ctcgcctttt acgtaatgga caaattcttc caactgatct
     1561 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1621 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1681 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1741 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1801 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1861 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1921 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1981 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     2041 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     2101 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc gctgtgtggc
     2161 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     2221 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     2281 tccctcatga tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     2341 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     2401 atagactgta ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     2461 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata ctcttccttt
     2521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2641 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2701 cctttcgtcc actgaagagc cactgtggtc tca
//
