LOCUS       sequence-495464-        2667 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2667)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30013
FEATURES             Location/Qualifiers
     source          1..2667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(508..525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(679..1267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(759..778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1438..2229)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2451..2555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2623..2641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggcggatgt gaacctggtg gaaagcggcg gcgcgctggt gaacccgggc
       61 ggcagcctgc gcctgagctg cgcggcgagc ggctttccgg tgcagcgcta tagcatgcgc
      121 tggtggcgca acgcgccggg caaagaacgc gaatgggtgg cgggcatgag cagcgcgggc
      181 gatcgcagca gctatgaaga tagcgtgaaa ggccgcttta ccattagccg cgatgatgcg
      241 cgcaacaccg tgtatctgaa catgaacagc ctgaaaccgg aagataccgc ggtgtattat
      301 tgcaacgtga acgtgggctt tgaatattgg ggcaacggca ccaacgtgac cgtgagcagc
      361 cgcaacagca ttagcagcct gagcattccg agcaccgtgc cgcgcgcgcg cgatccgccg
      421 gtggattcaa tgtgagacca cgagtgatta atgaatcggc caacgcgcgg ggagaggcgg
      481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
      601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
      661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1441 tttgccgact accttggtga tctcgccttt tacgtaatgg acaaattctt ccaactgatc
     1501 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
     1561 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
     1621 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
     1681 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
     1741 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
     1801 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
     1861 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
     1921 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
     1981 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
     2041 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cgctgtgtgg
     2101 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
     2161 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
     2221 ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
     2281 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
     2341 catagactgt accccaaaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     2401 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat actcttcctt
     2461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2581 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2641 ccctttcgtc cactgaagag ccactgt
//
