LOCUS       sequence-495465-        2929 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2929)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50069
FEATURES             Location/Qualifiers
     source          1..2929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..689
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTL
                     QYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHL"
     primer_bind     complement(729..747)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        815..919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1141..1932
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2103..2691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2592..2611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2845..2862
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggtgtct accggtgagg aacttttcac tggtgtggtg cctttcaagt
       61 tccagcttaa gggcaccatc aacggcaagt ctttcaccgt tgaaggtgaa ggtgagggca
      121 attctcatga gggttctcac aagggaaagt acgtgtgtac ctctggcaag ctgcctatgt
      181 cttgggctgc tcttggtact tctttcggct acggcatgaa gtactacact aagtacccaa
      241 gcggcctgaa gaactggttc catgaagtta tgcctgaggg cttcacctac gataggcaca
      301 tccagtacaa aggcgacggt agcattcatg ctaagcacca gcatttcatg aagaacggca
      361 cctaccacaa catcgttgag ttcaccggcc aggacttcaa agagaactct cctgttctga
      421 ccggcgatat ggatgtgtct ttgcctaatg aggtgcagca catccctatc gatgatggtg
      481 ttgagtgcac tgtgaccctt cagtaccctc tgctttccga tgagtctaag tgcgttgagg
      541 cttaccagaa caccattatc aagccgcttc acaaccagcc tgctccagat gttccattcc
      601 actggattag gaagcagtac acccagagca aggatgacac tgaagagagg gatcacatca
      661 tccagagcga gactcttgag gctcaccttt aggctttgag accacgtggc tcttcagtgg
      721 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      781 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      901 atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      961 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta
     1021 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     1081 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     1141 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     1201 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     1261 ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     1321 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     1381 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     1441 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     1501 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     1561 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     1621 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     1681 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     1741 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     1801 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     1861 tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta
     1921 gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     2881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactgt
//
