LOCUS       sequence-495469-        2848 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part YAO promoter, GGAG - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2848)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL12039
FEATURES             Location/Qualifiers
     source          1..2848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1475..1493)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1561..1665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1887..2678
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 tcgacaaaga ctggtcggtc ggttttggta gacaattgaa attagatgga tggtccggtt
      901 cggtatacta taagattaaa aacagtttta aattcagcta aaccgaactc atttgatttt
      961 attaaaccgg aatcatccga ttcgagtttg taaaaaatac cgaaattgaa aacactaaac
     1021 aaaaactgta ttaaactgtt actgaaataa gagaatctcc caattcggtt tacgtactac
     1081 tcttcagaaa tcagaaccaa aaattcagaa atcggattga accaaactta aattgacggt
     1141 ccggttagtt ttcggctcta caaattaaag gcccaagttt ctgctttaaa agaacgaaat
     1201 agttaatggg ctcaaaccat agaccaggta agtcatgggc ttggttagtc cgggtcaacc
     1261 cggtagaccc gattcctgaa gaaaacctag tggaaggttt aaagttgtaa actttccgac
     1321 caaataaaca aaatcgtttt ccagcttctt ccgtcgccac taaaccctga ggctaaacct
     1381 agacgagtca aagtgtaaaa tcgttaaacc ctaagaggga gtgagagaga gaagaaatgt
     1441 gagaccacga agtggctctt cagtggacga aagggcctcg tgatacgcct atttttatag
     1501 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1561 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1621 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgcg ctcacgcaac
     1681 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     1741 tgttatgact gttttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     1801 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     1861 gtcgccctaa aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc
     1921 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     1981 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagcgatatt gatttgctgg
     2041 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2101 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2161 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2221 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2281 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2341 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2401 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2461 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2521 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2581 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccatt
     2641 acgtaaaagg cgagatcacc aaggtagtcg gcaaataact gtcagaccaa gtttactcat
     2701 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2761 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2821 accccgtaga aaagatcaaa ggatcttc
//
