LOCUS       sequence-495470-        2769 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2769)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30031
FEATURES             Location/Qualifiers
     source          1..2769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             412..435
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             460..483
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             508..531
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(610..627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(781..1369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(861..880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1540..2331)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2553..2657)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2725..2743
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggagagtaa acgtaataag ccaggtaagg caacaggtaa aggtaaacca
       61 gttggtgata aatggcttga tgatgcaggt aaagattcag gagcaccaat tccagatcgt
      121 attgctgata agttgcgtga taaagaattt aaaagcttcg acgattttcg taaggctgtt
      181 tgggaagagg tgtcaaagga tcctgagctt agtaaaaatt tgaacccaag caataagtct
      241 agtgtttcaa aaggttattc tccatttact ccaaagaatc aacaggtcgg aggtagaaaa
      301 gtctatgaac ttcatgctga caagccaatt agtcaaggtg gtgaggttta tgacatggat
      361 aatatcagag tgactacacc taagagacat atcgatattc accgtggtaa gtggagccac
      421 cctcagtttg agaaaggtgg aggaagcggt ggatcagctt ggtcacaccc tcaattcgag
      481 aagggtggtt ctggaggtgg tgctagcctt gaggttttgt tccagggtcc aatgtgagac
      541 cacgagtgat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      601 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1501 atcaatctaa agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt
     1561 gatctcgcct tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     1621 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     1681 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     1741 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     1801 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     1861 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     1921 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     1981 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     2041 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     2101 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     2161 ccttacggtc accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac
     2221 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     2281 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
     2341 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     2401 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     2461 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     2521 gtcaaggttc tggaccagtt gcgtgagcgc atactcttcc tttttcaata ttattgaagc
     2581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2701 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tccactgaag
     2761 agccactgt
//
