LOCUS       sequence-495473-        2472 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2472)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30040
FEATURES             Location/Qualifiers
     source          1..2472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..69
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             109..198
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             139..228
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(313..330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(484..1072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(564..583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1243..2034)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2256..2360)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2428..2446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgggctcggg ccgtggcgtg cctcatatcg tgatggtgga cgcctacaag
       61 cgttacaagt cgggctcgga gaatttgtat tttcagggtt cgggctcgga acaaaagttg
      121 atctctgaag aggaccttga gcagaaattg atctctgagg aagatcttga gcagaagctt
      181 atctcagagg aggatttgga gcaaaaactt atttctgaag aggacctttc gggaatgtga
      241 gaccacgagt gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      481 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      841 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      901 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      961 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1021 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1081 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1141 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1201 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttatttgc cgactacctt
     1261 ggtgatctcg ccttttacgt aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     1321 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     1381 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     1441 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     1501 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     1561 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     1621 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     1681 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     1741 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     1801 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1861 aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca ggccgccatc
     1921 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1981 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
     2041 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     2101 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     2161 aaaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2221 tcggtcaagg ttctggacca gttgcgtgag cgcatactct tcctttttca atattattga
     2281 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2341 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2401 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccactg
     2461 aagagccact gt
//
