LOCUS       sequence-495474-        2469 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2469)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30041
FEATURES             Location/Qualifiers
     source          1..2469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..69
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     CDS             76..99
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             106..195
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             136..225
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(310..327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(481..1069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(561..580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1240..2031)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2253..2357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2425..2443
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgggctcggg ccgtggcgtg cctcatatcg tgatggtgga cgcctacaag
       61 cgttacaagt cgggcctgga agttctgttt cagggcccat cgtcggaaca aaagttgatc
      121 tctgaagagg accttgagca gaaattgatc tctgaggaag atcttgagca gaagcttatc
      181 tcagaggagg atttggagca aaaacttatt tctgaagagg acctttcggg aatgtgagac
      241 cacgagtgat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      301 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1201 atcaatctaa agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt
     1261 gatctcgcct tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     1321 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     1381 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     1441 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     1501 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     1561 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     1621 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     1681 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     1741 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     1801 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     1861 ccttacggtc accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac
     1921 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     1981 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
     2041 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     2101 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     2161 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     2221 gtcaaggttc tggaccagtt gcgtgagcgc atactcttcc tttttcaata ttattgaagc
     2281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2401 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tccactgaag
     2461 agccactgt
//
