LOCUS       sequence-495478-        2352 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2352)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30050
FEATURES             Location/Qualifiers
     source          1..2352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             85..108
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(193..210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(364..952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(444..463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1123..1914)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2136..2240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2308..2326
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggatgtatt caagaaagga ttttcaatag ccgatgaggg agttgtggga
       61 gctgttgaga gcagcggtag cggtctggaa gttctgtttc agggcccagg agcaatgtga
      121 gaccacgagt gattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
      181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttatttgc cgactacctt
     1141 ggtgatctcg ccttttacgt aatggacaaa ttcttccaac tgatctgcgc gcgaggccaa
     1201 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
     1261 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
     1321 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
     1381 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
     1441 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
     1501 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
     1561 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
     1621 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
     1681 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
     1741 aagccttacg gtcaccgtaa ccagcaaatc aatatcgctg tgtggcttca ggccgccatc
     1801 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
     1861 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatgatgtt
     1921 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1981 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     2041 aaaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     2101 tcggtcaagg ttctggacca gttgcgtgag cgcatactct tcctttttca atattattga
     2161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2281 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccactg
     2341 aagagccact gt
//
