LOCUS       sequence-495480-        2445 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2445)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30052
FEATURES             Location/Qualifiers
     source          1..2445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             142..171
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             181..204
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(286..303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(457..1045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(537..556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1216..2007)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2229..2333)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2401..2419
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggatgtatt catgaaagga ctttcaaagg ccaaggaggg agttgtggca
       61 gctgctgaga aaaccaaaca gggtgtggca gaagcagcag gaaagacaaa agagggtgtt
      121 ctcagcagcg gtagcggtgc acatcatcac caccatcacc atcatcacca tagcagcggt
      181 ctggaagttc tgtttcaggg cccagcaatg tgagaccacg agtgattaat gaatcggcca
      241 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      301 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      361 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      421 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      481 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      541 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      601 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      661 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      721 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      781 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      841 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      901 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      961 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1021 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1081 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1141 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1201 aacttggtct gacagttatt tgccgactac cttggtgatc tcgcctttta cgtaatggac
     1261 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     1321 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     1381 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     1441 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     1501 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     1561 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     1621 aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc
     1681 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     1741 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     1801 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     1861 atcaatatcg ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     1921 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     1981 tacttcggcg atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg
     2041 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     2101 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa aacagtcata acaagccatg
     2161 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     2221 gagcgcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2341 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2401 aataggcgta tcacgaggcc ctttcgtcca ctgaagagcc actgt
//
