LOCUS       sequence-495482-        2968 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2968)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50068
FEATURES             Location/Qualifiers
     source          1..2968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(318..337)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(768..786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        854..958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1180..1971
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2142..2730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2631..2650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2884..2901
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggtgagc aagggcgaag agttgttcac tggtgtggtg cctattctgg
       61 ttgagctgga tggtgatgtg aacggccata agttctccgt gtctggtgaa ggtgagggtg
      121 atgctactta cggtaagctg accctgaagt tcatctgtac caccggtaag ttgcctgtgc
      181 cttggcctac tcttgtgact actctttcat ggggtgtgca gtgcttcgct aggtatcctg
      241 atcatatgaa gcagcacgac ttcttcaaga gcgctatgcc tgagggttac gtgcaagaga
      301 ggaccatttt cttcaaggac gacggcaact acaagaccag agctgaggtt aagttcgagg
      361 gtgataccct ggtgaacagg attgagctga agggcatcga cttcaaagag gatggtaaca
      421 tcctgggcca caagctcgag tacaactact tcagcgacaa cgtgtacatc accgccgaca
      481 agcagaagaa cggtatcaag gccaacttca agatccggca caacatcgag gatggtggtg
      541 ttcagcttgc tgatcactac cagcagaaca cccctattgg tgatggtcct gtgcttctgc
      601 ctgataacca ctaccttagc acccagagca agctgtccaa ggatcctaat gagaagaggg
      661 accacatggt tctgcttgag tttgttaccg ctgctggtat taccctcggt atggatgagc
      721 tgtacaagta ggctttgaga ccacgtggct cttcagtgga cgaaagggcc tcgtgatacg
      781 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      841 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      901 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      961 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1021 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     1081 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1141 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     1201 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1261 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     1321 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1381 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     1441 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1501 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     1561 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1621 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1681 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1741 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1801 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     1861 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     1921 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2281 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2521 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2581 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2641 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2701 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2761 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2821 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2881 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2941 gcgcgttggc cgattcatta atcactgt
//
