LOCUS       sequence-495485-        3941 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part RPS5a promoter, GGAG - CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL13019
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2568..2586)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2654..2758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2980..3771
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 ctccccataa tcgtgagtag atatattact caacttttga ttcgctattt gcagtgcacc
      901 tgtggcgttc atcacatctt ttgtgacact gtttgcactg gtcattgcta ttacaaagga
      961 ccttcctgat gttgaaggag atcgaaagta agtaactgca cgcataacca ttttctttcc
     1021 gctctttggc tcaatccatt tgacagtcaa agacaatgtt taaccagctc cgtttgatat
     1081 attgtcttta tgtgtttgtt caagcatgtt tagttaatca tgcctttgat tgatcttgaa
     1141 taggttccaa atatcaaccc tggcaacaaa acttggagtg agaaacattg cattcctcgg
     1201 ttctggactt ctgctagtaa attatgtttc agccatatca ctagctttct acatgcctca
     1261 ggtgaattca tctatttccg tcttaactat ttcggttaat taaagcacga acaccattac
     1321 tgcatgtaga agcttgataa actatcgcca ccaatttatt tttgttgcga tattgttact
     1381 ttcctcagta tgcagctttg aaaagaccaa ccctcttatc ctttaacaat gaacaggttt
     1441 ttagaggtag cttgatgatt cctgcacatg tgatcttggc ttcaggctta attttccagg
     1501 taaagcatta tgagatactc ttatatctct tacatacttt tgagataatg cacaagaact
     1561 tcataactat atgctttagt ttctgcattt gacactgcca aattcattaa tctctaatat
     1621 ctttgttgtt gatctttggt agacatgggt actagaaaaa gcaaactaca ccaaggtaaa
     1681 atacttttgt acaaacataa actcgttatc acggaacatc aatggagtgt atatctaacg
     1741 gagtgtagaa acatttgatt attgcaggaa gctatctcag gatattatcg gtttatatgg
     1801 aatctcttct acgcagagta tctgttattc cccttcctct agctttcaat ttcatggtga
     1861 ggatatgcag ttttctttgt atatcattct tcttcttctt tgtagcttgg agtcaaaatc
     1921 ggttccttca tgtacataca tcaaggatat gtccttctga atttttatat cttgcaataa
     1981 aaatgcttgt accaattgaa acaccagctt tttgagttct atgatcactg acttggttct
     2041 aaccaaaaaa aaaaaaatgt ttaatttaca tatctaaaag taggtttagg gaaacctaaa
     2101 cagtaaaata tttgtatatt attcgaattt cactcatcat aaaaacttaa attgcaccat
     2161 aaaattttgt tttactatta atgatgtaat ttgtgtaact taagataaaa ataatattcc
     2221 gtaagttaac cggctaaaac cacgtataaa ccagggaacc tgttaaaccg gttctttact
     2281 ggataaagaa atgaaagccc atgtagacag ctccattaga gcccaaaccc taaatttctc
     2341 atctatataa aaggagtgac attagggttt ttgttcgtcc tcttaaagct tctcgttttc
     2401 tctgccgtct ctctcattcg cgcgacgcaa acgatcttca ggtgatcttc tttctccaaa
     2461 tcctctctca taactctgat ttcgtacttg tgtatttgag ctcacgctct gtttctctca
     2521 ccacagcccc attgagacca cgaagtggct cttcagtgga cgaaagggcc tcgtgatacg
     2581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2761 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     2821 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     2881 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2941 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     3001 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     3061 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     3121 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     3181 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     3241 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     3301 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     3361 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     3421 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     3481 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     3541 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     3601 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     3661 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     3721 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     3781 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3841 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3901 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
