LOCUS       sequence-495486-        3611 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, 
            GGAG - CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3611)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL13020
FEATURES             Location/Qualifiers
     source          1..3611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2238..2256)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2324..2428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2650..3441
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcaggag
      841 gaataaaagc atttgcgttt ggtttatcat tgcgtttata caaggacaga gatccactga
      901 gctggaatag cttaaaacca ttatcagaac aaaataaacc attttttgtt aagaatcaga
      961 gcatagtaaa caacagaaac aacctaagag aggtaacttg tccaagaaga tagctaatta
     1021 tatctatttt ataaaagtta tcatagtttg taagtcacaa aagatgcaaa taacagagaa
     1081 actaggagac ttgagaatat acattcttgt atatttgtat tcgagattgt gaaaatttga
     1141 ccataagttt aaattcttaa aaagatatat ctgatctagg tgatggttat agactgtaat
     1201 tttaccacat gtttaatgat ggatagtgac acacatgaca catcgacaac actatagcat
     1261 cttatttaga ttacaacatg aaatttttct gtaatacatg tctttgtaca taatttaaaa
     1321 gtaattccta agaaatatat ttatacaagg agtttaaaga aaacatagca taaagttcaa
     1381 tgagtagtaa aaaccatata cagtatatag cataaagttc aatgagttta ttacaaaagc
     1441 attggttcac tttctgtaac acgacgttaa accttcgtct ccaataggag cgctactgat
     1501 tcaacatgcc aatatatact aaatacgttt ctacagtcaa atgctttaac gtttcatgat
     1561 taagtgacta tttaccgtca atcctttccc attcctccca ctaatccaac tttttaatta
     1621 ctcttaaatc accactaagc tagtaacgcc tatcatgaat tagctctact aaatctagca
     1681 acctttcaaa tttgcagtat tgcaggtgtc tctgtgtctt taaaatagtt gccttatgat
     1741 ttcttcggtt tcaagatgat caaatagtta tagatttcat gctcacacat gctcattaga
     1801 tgtgtacata ctttacttac ccaaatctat tttctcgcaa agattttgat ggtaaagctg
     1861 atttggttct attgaactaa atcaaacgag tttcagactg agtgattcta atccggccca
     1921 ttagccccta aacagaccca ctaattacgc agcttttaat agagtaatta cacctagttt
     1981 acccactaaa ccactaagca ctaattatct cacaatctaa tgagcttccc tcgtaattac
     2041 ttgggctttc actctaccat ttatttgtaa cagtcaagtc tctactgtct ctatataaac
     2101 tctctaaagt taacacacaa ttctcatcac aaacaaatca accaaagcaa cttctactct
     2161 ttcttctttc gaccttatca atctgttgag aaatctagcc attgagacca cgaagtggct
     2221 cttcagtgga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2281 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2341 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2401 gcttcaataa tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac
     2461 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2521 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     2581 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     2641 ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     2701 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     2761 gtggatggcg gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg
     2821 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     2881 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     2941 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     3001 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     3061 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     3121 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     3181 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     3241 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     3301 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     3361 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc
     3421 accaaggtag tcggcaaata actgtcagac caagtttact catatatact ttagattgat
     3481 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3541 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3601 aaaggatctt c
//
