LOCUS       sequence-495487-        2673 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part pUbiquitin (At5g42300) terminator, GCTT - 
            CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2673)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60002
FEATURES             Location/Qualifiers
     source          1..2673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1300..1318)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1386..1490
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1712..2503
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 caatcgaagg agctaagctg aaggagtgtt gttgatttgg acacctacat aaactcatat
      901 caaccatgtt acatctaaaa aagacacaag tatgttgtaa gacgtgataa cttcaaattt
      961 gtggtaataa taatacattc actgtcaatg gttttaaacc cttccaaatt gtcagattga
     1021 tttctggtta tctctaagtt tgtagtatat tagtcttttt ggttgtattg atgtgctctt
     1081 tctccaaatg ttacccatct tccaaatacc actagtgctt attgcttact gcttaccact
     1141 gattagacac caaacttgta ggcctttaag cagtgcaata tgaatgcaag aacagatttt
     1201 ctagcttaag acgctctaga aatcagtttc ttgtttaaga attaagatac tctcactagt
     1261 cgcttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
     1321 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1381 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1441 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
     1501 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1561 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
     1621 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     1681 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
     1741 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     1801 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
     1861 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     1921 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     1981 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     2041 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     2101 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     2161 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     2221 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     2281 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     2341 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     2401 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     2461 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     2521 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2581 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2641 gtcagacccc gtagaaaaga tcaaaggatc ttc
//
