LOCUS       sequence-495488-        2691 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Oleosin terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2691)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60005
FEATURES             Location/Qualifiers
     source          1..2691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1318..1336)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1404..1508
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1730..2521
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 accccactga tgtcatcgtc atagtccaat aactccaatg tcggggagtt agtttatgag
      901 gaataaagtg tttagaattt gatcaggggg agataataaa agccgagttt gaatcttttt
      961 gttataagta atgtttatgt gtgtttctat atgttgtcaa atggtaccat gttttttttc
     1021 ctctcttttt gtaacttgca agtgttgtgt tgtactttat ttggcttctt tgtaagttgg
     1081 taacggtggt ctatatatgg aaaaggtctt gttttgttaa acttatgtta gttaactgga
     1141 ttcgtcttta accacaaaaa gttttcaata agctacaaat ttagacacgc aagccgatgc
     1201 agtcattagt acatatattt attgcaagtg attacatggc aacccaaact tcaaaaacag
     1261 taggttgctc catttagtcg cttgagacca cgaagtggct cttcagtgga cgaaagggcc
     1321 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1381 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1441 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1501 ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1561 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc
     1621 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     1681 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
     1741 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1801 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1861 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     1921 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     1981 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2041 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2101 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2161 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2221 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2281 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2341 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2401 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2461 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     2521 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2581 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2641 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
