LOCUS       sequence-495489-        2658 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2658)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30062
FEATURES             Location/Qualifiers
     source          1..2658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(499..516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(670..1258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(750..769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1429..2220)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2442..2546)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2614..2632
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tggcaaagta tgatagcgaa gtgaaccagg aagcgaaacc ggaagttaaa
       61 ccggaagtga aaccggaaac ccatattaat ctgaaagtta gcgacggcag cagcgaaatc
      121 ttttttaaaa ttaaaaaaac caccccgctg cgtcgcctga tggaagcctt tgcgaaacgt
      181 cagggtaaag aaatggatag cctgcgcttt ctgtatgacg gcatccgtat tcaggccgat
      241 cagaccccgg aagatctgga tatggaagat aacgatatta ttgaagcgca tcgcgaacag
      301 atcagcagcg gcgtgaaaat tgtgagccgc aaaagcctgg gcgtgcagaa tgtgtatgat
      361 attggcgtgg aaaaagatca taactttctg ctggcgaatg gcctgattgc gagcaacgga
      421 atgtgagacc acgagtgatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      481 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1081 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1321 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1381 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt atttgccgac
     1441 taccttggtg atctcgcctt ttacgtaatg gacaaattct tccaactgat ctgcgcgcga
     1501 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     1561 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     1621 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     1681 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     1741 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     1801 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     1861 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     1921 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     1981 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     2041 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcgctgtgtg gcttcaggcc
     2101 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     2161 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     2221 gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
     2281 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
     2341 taccccaaaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2401 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tactcttcct ttttcaatat
     2461 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2521 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2581 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2641 ccactgaaga gccactgt
//
