LOCUS       sequence-495494-        2934 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2934)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30068
FEATURES             Location/Qualifiers
     source          1..2934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..663
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     621..643
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             673..696
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(775..792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(946..1534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1026..1045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1705..2496)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2718..2822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2890..2908
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtctcacca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact
       61 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa
      121 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat
      181 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac
      241 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga
      301 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact
      361 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta
      421 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac
      481 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt
      541 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag
      601 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca
      661 aaatcggatg gtctggaagt tctgtttcag ggcccaatgt gagaccacga gtgattaatg
      721 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1381 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1681 atatgagtaa acttggtctg acagttattt gccgactacc ttggtgatct cgccttttac
     1741 gtaatggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     1801 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     1861 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     1921 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     1981 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     2041 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     2101 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     2161 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     2221 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     2281 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
     2341 aaccagcaaa tcaatatcgc tgtgtggctt caggccgcca tccactgcgg agccgtacaa
     2401 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
     2461 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg
     2521 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     2581 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaaa acagtcataa
     2641 caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac
     2701 cagttgcgtg agcgcatact cttccttttt caatattatt gaagcattta tcagggttat
     2761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2821 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2881 acctataaaa ataggcgtat cacgaggccc tttcgtccac tgaagagcca ctgt
//
