LOCUS       sequence-495495-        3132 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Rb7MAR (D144-437 variant) terminator, GCTT 
            - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3132)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60025
FEATURES             Location/Qualifiers
     source          1..3132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1759..1777)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1845..1949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2171..2962
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 tcgattaaaa atcccaatta tatttggtct aatttagttt ggtattgagt aaaacaaatt
      901 cgaaccaaac caaaatataa atatatagtt tttatatata tgcctttaag actttttata
      961 gaattttctt taaaaaatat ctaggtacat caacgaaaaa ttagtcaaac gactaaaata
     1021 aataaatatc atgtgttatt aagaaaattc tcctataaga atattttaat agatcatatg
     1081 tttgtaaaaa aaattaattt ttactaacac atatatttac ttatcaaaaa tttgacaaag
     1141 taagattaaa ataatattca tctaacaaaa aaaaaaccag aaaatgctga aaacccggca
     1201 aaaccgaacc aatccaaacc gatatagttg gtttggtttg attttgatat aaaccgaacc
     1261 aactcggtcc atttgcaccc ctaatcataa tagctttaat atttcaagat attattaagt
     1321 taacgttgtc aatatcctgg aaattttgca aaatgaatca agcctatatg gctgtaatat
     1381 gaatttaaaa gcagctcgat gtggtggtaa tatgtaattt acttgattct aaaaaaatat
     1441 cccaagtatt aataatttct gctaggaaga aggttagcta cgatttacag caaagccaga
     1501 atacaaagaa ccataaagtg attgaagctc gaaatatacg aaggaacaaa tatttttaaa
     1561 aaaatacgca atgacttgga acaaaagaaa gtgatatatt ttttgttctt aaacaagcat
     1621 cccctctaaa gaatggcagt tttcctttgc atgtaactat tatgctccct tcgttacaaa
     1681 aattttggac tactattggg aacttcttct gaaaatagtc gcttgagacc acgaagtggc
     1741 tcttcagtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1801 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1921 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
     1981 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2041 ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg
     2101 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa
     2161 gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
     2221 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
     2281 agtggatggc ggcctgaagc cacacagcga tattgatttg ctggttacgg tgaccgtaag
     2341 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     2401 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     2461 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     2521 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     2581 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     2641 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     2701 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     2761 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     2821 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     2881 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cattacgtaa aaggcgagat
     2941 caccaaggta gtcggcaaat aactgtcaga ccaagtttac tcatatatac tttagattga
     3001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3121 caaaggatct tc
//
