LOCUS       sequence-495496-        3776 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Extensin-Truncated-EU + NbACT terminator, 
            GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3776)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60026
FEATURES             Location/Qualifiers
     source          1..3776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2403..2421)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2489..2593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2815..3606
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 caaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac aatgagaaaa
      901 gaagaagaag aagaagaaga agaagaagaa gaaaagagta aataataaag ccccacagga
      961 ggcgaagttc ttgtagctcc atgttatcta agttattgat attgtttgcc ctatatttta
     1021 tttctgtcat tgtgtatgtt ttgttcagtt tcgatctcct tgcaaaatgc agagattatg
     1081 agatgaataa actaagttat attattatac gtgttaatat tctcctcctc tctctagcta
     1141 gccttttgtt ttctcttttt cttatttgat tttctttaaa tcaatccatt ttaggagagg
     1201 gccagggagt gatccagcaa aacatgaaga ttagaagaaa cttccctctt ttttttcctg
     1261 aaaacaattt aacgtcgaga tttatctctt tttgtaatgg aatcatttct acagttatga
     1321 ctagaataca gcattcccag aaagagaaac agaagaaata tacaaacttt cattttgaga
     1381 gcagcacctc gtctattgat tgcagataat atgcttctca tttgtatttc cttttgatta
     1441 tttttgtttc tatccctttg tttgagtcaa tctcaaatat tcggtcattg ttggtatgaa
     1501 aaatcaagca gttcatgtta agagtcaatt taaaattaat atttttatat agagttgtat
     1561 gtgaaatgat gttgtgattt ggtatatatg gataaagagc ttgtcagttc atttttgtct
     1621 catttttttg gtatccaaat aagaaacaca aaagggatat gtccctctac tatcaaatat
     1681 tagttataag tattcatgtt atactattcg atattttcta ccccaatcgt tacctattta
     1741 aaagtattta cccctccatc tatcaaaccc ctggacccag ctttcctatt acatgtggct
     1801 tcatcttaag cccccaaacc tttttcttat ttttgatttt taaaggctca tcttaaaatt
     1861 tattactcaa attaatacct cttaataacc cacctcaagg acccagtaat taaatatcca
     1921 attagctcca gtaattgggg ttcatattag ctccagtctt aaattttaaa ggcgatgatc
     1981 gtattcctcc acttggttca tttatactca aagaatactc aatgtcttta gtgtttagat
     2041 aactttttgt aaatcatata gattgtttta acaaaaaaca attcaatagt agattttcac
     2101 atgaaagtta cataaaaatt ctttaaaatt actttctcaa aaaattgttc caaacatatt
     2161 atcccacaat taaactcaat ctgtttttcg aaacctaaat caaaaccaat ccaactacct
     2221 tatataatat ataatcaata cattgtaaag aactgcatgt tcttttaaat tttgggggca
     2281 aagttattcc gtacgttcac acatgtacta ataggaggta ataaatgata tgtgaaacaa
     2341 tcgaggtgta aacaagctag catcgcttga gaccacgaag tggctcttca gtggacgaaa
     2401 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2461 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2521 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2581 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     2641 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     2701 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     2761 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     2821 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     2881 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     2941 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     3001 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     3061 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     3121 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     3181 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     3241 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     3301 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     3361 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     3421 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     3481 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     3541 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     3601 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
