LOCUS       sequence-495497-        4655 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part Extensin-Truncated-EU + NbACT + Rb7MAR 
            (D144-437 variant) terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60027
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3282..3300)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3368..3472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             3694..4485
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 caaagcagaa tgctgagcta aaagaaaggc tttttccatt ttcgagagac aatgagaaaa
      901 gaagaagaag aagaagaaga agaagaagaa gaaaagagta aataataaag ccccacagga
      961 ggcgaagttc ttgtagctcc atgttatcta agttattgat attgtttgcc ctatatttta
     1021 tttctgtcat tgtgtatgtt ttgttcagtt tcgatctcct tgcaaaatgc agagattatg
     1081 agatgaataa actaagttat attattatac gtgttaatat tctcctcctc tctctagcta
     1141 gccttttgtt ttctcttttt cttatttgat tttctttaaa tcaatccatt ttaggagagg
     1201 gccagggagt gatccagcaa aacatgaaga ttagaagaaa cttccctctt ttttttcctg
     1261 aaaacaattt aacgtcgaga tttatctctt tttgtaatgg aatcatttct acagttatga
     1321 ctagaataca gcattcccag aaagagaaac agaagaaata tacaaacttt cattttgaga
     1381 gcagcacctc gtctattgat tgcagataat atgcttctca tttgtatttc cttttgatta
     1441 tttttgtttc tatccctttg tttgagtcaa tctcaaatat tcggtcattg ttggtatgaa
     1501 aaatcaagca gttcatgtta agagtcaatt taaaattaat atttttatat agagttgtat
     1561 gtgaaatgat gttgtgattt ggtatatatg gataaagagc ttgtcagttc atttttgtct
     1621 catttttttg gtatccaaat aagaaacaca aaagggatat gtccctctac tatcaaatat
     1681 tagttataag tattcatgtt atactattcg atattttcta ccccaatcgt tacctattta
     1741 aaagtattta cccctccatc tatcaaaccc ctggacccag ctttcctatt acatgtggct
     1801 tcatcttaag cccccaaacc tttttcttat ttttgatttt taaaggctca tcttaaaatt
     1861 tattactcaa attaatacct cttaataacc cacctcaagg acccagtaat taaatatcca
     1921 attagctcca gtaattgggg ttcatattag ctccagtctt aaattttaaa ggcgatgatc
     1981 gtattcctcc acttggttca tttatactca aagaatactc aatgtcttta gtgtttagat
     2041 aactttttgt aaatcatata gattgtttta acaaaaaaca attcaatagt agattttcac
     2101 atgaaagtta cataaaaatt ctttaaaatt actttctcaa aaaattgttc caaacatatt
     2161 atcccacaat taaactcaat ctgtttttcg aaacctaaat caaaaccaat ccaactacct
     2221 tatataatat ataatcaata cattgtaaag aactgcatgt tcttttaaat tttgggggca
     2281 aagttattcc gtacgttcac acatgtacta ataggaggta ataaatgata tgtgaaacaa
     2341 tcgaggtgta aacaagctag cattcgatta aaaatcccaa ttatatttgg tctaatttag
     2401 tttggtattg agtaaaacaa attcgaacca aaccaaaata taaatatata gtttttatat
     2461 atatgccttt aagacttttt atagaatttt ctttaaaaaa tatctaggta catcaacgaa
     2521 aaattagtca aacgactaaa ataaataaat atcatgtgtt attaagaaaa ttctcctata
     2581 agaatatttt aatagatcat atgtttgtaa aaaaaattaa tttttactaa cacatatatt
     2641 tacttatcaa aaatttgaca aagtaagatt aaaataatat tcatctaaca aaaaaaaaac
     2701 cagaaaatgc tgaaaacccg gcaaaaccga accaatccaa accgatatag ttggtttggt
     2761 ttgattttga tataaaccga accaactcgg tccatttgca cccctaatca taatagcttt
     2821 aatatttcaa gatattatta agttaacgtt gtcaatatcc tggaaatttt gcaaaatgaa
     2881 tcaagcctat atggctgtaa tatgaattta aaagcagctc gatgtggtgg taatatgtaa
     2941 tttacttgat tctaaaaaaa tatcccaagt attaataatt tctgctagga agaaggttag
     3001 ctacgattta cagcaaagcc agaatacaaa gaaccataaa gtgattgaag ctcgaaatat
     3061 acgaaggaac aaatattttt aaaaaaatac gcaatgactt ggaacaaaag aaagtgatat
     3121 attttttgtt cttaaacaag catcccctct aaagaatggc agttttcctt tgcatgtaac
     3181 tattatgctc ccttcgttac aaaaattttg gactactatt gggaacttct tctgaaaata
     3241 gtcgcttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt
     3301 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3361 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3421 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc
     3481 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     3541 catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag
     3601 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     3661 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
     3721 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     3781 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
     3841 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     3901 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     3961 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     4021 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     4081 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     4141 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     4201 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     4261 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     4321 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     4381 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     4441 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
     4501 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4561 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4621 gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
