LOCUS       sequence-495499-        2881 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate partAtUbi10 terminator, GCTT - CGCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  TITLE     Plant Golden-gate Promoters, Terminators and Level0/Level1 cloning 
            acceptors.
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL60038
FEATURES             Location/Qualifiers
     source          1..2881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1508..1526)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1594..1698
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1920..2711
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg gtctcagctt
      841 atctcgtctc tgttatgctt aagaagttca atgtttcgtt tcatgtaaaa ctttggtggt
      901 ttgtgttttg gggccttgta taatccctga tgaataagtg ttctactatg tttccgttcc
      961 tgttatctct ttctttctaa tgacaagtcg aacttcttct ttatcatcgc ttcgttttta
     1021 ttatctgtgc ttcttttgtt taatacgcct gcaaagtgac tcgactctgt ttagtgcagt
     1081 tctgcgaaac ttgtaaatag tccaattgtt ggcctctagt aatagatgta gcgaaagtgt
     1141 tgagctgttg ggttctaagg atggcttgaa catgttaatc ttttaggttc tgagtatgat
     1201 gaacattcgt tgttgctaag aaatgcctgt aatgtcccac aaatgtagaa aatggttcgt
     1261 acctttgtcc aagcattgat atgtctgatg agaggaaact gcaagatact gagcttggtt
     1321 taacgaagga gaggcagttt cttccttcca aagcatttca tttgacaatg ccttgatcat
     1381 cttaagtaga gtttctgttg tggaaagttt gaaactttga agaaacgact ctcaagtaaa
     1441 ttgatgatca caagtgaaag tgtatgttcg cttgagacca cgaagtggct cttcagtgga
     1501 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1561 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     1621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     1681 tattgaaaaa ggaagagtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1741 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tttggggtac
     1801 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1861 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1921 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1981 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     2041 gcctgaagcc acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     2101 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     2161 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     2221 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     2281 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     2341 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     2401 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     2461 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2521 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2581 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2641 cgcgcgcaga tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag
     2701 tcggcaaata actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2881 c
//
