LOCUS       sequence-495500-        2734 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2734)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50003
FEATURES             Location/Qualifiers
     source          1..2734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(30..32,33..35,36..494)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(75..96)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(336..355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(534..552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        620..724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             946..1737
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1908..2496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2397..2416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2650..2667
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gaacgcggct attagatcaa tggtgagcaa gggcgaggag ctgttcaccg
       61 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca
      181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt
      241 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      481 tctatatcat ggcctgagct ttgagaccac gtggctcttc agtggacgaa agggcctcgt
      541 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      601 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      661 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      721 gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
      781 agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg
      841 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      901 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
      961 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     1021 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     1081 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     1141 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     1201 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     1261 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     1321 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     1381 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     1441 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     1501 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     1561 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     1621 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     1681 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
     1741 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2041 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     2701 ctccccgcgc gttggccgat tcattaatca ctgt
//
