LOCUS       sequence-495516-        2812 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2812)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50043
FEATURES             Location/Qualifiers
     source          1..2812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..92
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             117..140
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(612..630)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        698..802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1024..1815
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1986..2574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2475..2494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2728..2745
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc ggattataag gaccatgacg gagactataa ggaccatgac ctcgacgctg
       61 cagcagctga ttataaggac gatgacgata agggtggttc tggaggtggt gctagccttg
      121 aggttttgtt ccagggtcct ggaagcggag gtggatcagg tggatcaatg gagagtaaac
      181 gtaataagcc aggtaaggca acaggtaaag gtaaaccagt tggtgataaa tggcttgatg
      241 atgcaggtaa agattcagga gcaccaattc cagatcgtat tgctgataag ttgcgtgata
      301 aagaatttaa aagcttcgac gattttcgta aggctgtttg ggaagaggtg tcaaaggatc
      361 ctgagcttag taaaaatttg aacccaagca ataagtctag tgtttcaaaa ggttattctc
      421 catttactcc aaagaatcaa caggtcggag gtagaaaagt ctatgaactt catgctgaca
      481 agccaattag tcaaggtggt gaggtttatg acatggataa tatcagagtg actacaccta
      541 agagacatat cgatattcac cgtggtaagt aatgagcttt gagaccacgt ggctcttcag
      601 tggacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      661 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      721 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      781 ataatattga aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      841 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg
      901 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
      961 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
     1021 atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
     1081 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
     1141 ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat
     1201 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
     1261 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
     1321 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
     1381 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
     1441 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
     1501 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
     1561 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
     1621 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
     1681 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
     1741 gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag
     1801 gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2761 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact gt
//
