LOCUS       sequence-495518-        3523 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3523)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50047
FEATURES             Location/Qualifiers
     source          1..3523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..93)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             1260..1283
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1323..1341)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1409..1513
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1735..2526
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2697..3285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3186..3205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3439..3456
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc ggaggacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag
       61 aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
      121 gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg
      181 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
      241 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
      301 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
      361 tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga
      421 acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct aaaacggatt
      481 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      541 aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt
      601 cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca
      661 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      721 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
      781 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc
      841 aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa
      901 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac
      961 ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac
     1021 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     1081 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     1141 ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta
     1201 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggg
     1261 actacaagga tgacgatgac aagtaagctt tgagaccacg tggctcttca gtggacgaaa
     1321 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     1381 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1441 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1501 aaaaaggaag agtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     1561 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttttgg ggtacagtct
     1621 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     1681 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg
     1741 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     1801 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     1861 aagccacaca gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     1921 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     1981 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     2041 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     2101 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     2161 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     2221 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     2281 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     2341 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     2401 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     2461 gcagatcagt tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc
     2521 aaataactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2581 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2641 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2701 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2761 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2821 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3241 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3301 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3361 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3421 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3481 aaaccgcctc tccccgcgcg ttggccgatt cattaatcac tgt
//
