LOCUS       sequence-495520-        2407 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2407)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50049
FEATURES             Location/Qualifiers
     source          1..2407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             30..47
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..101
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             102..167
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(207..225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        293..397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             619..1410
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1581..2169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2070..2089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2323..2340
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggttccgga agaggatcgc atcaccatca ccatcacgat tatgatattc
       61 caactactgc tagcggcctg gaagttctgt ttcagggccc agactacaaa gaccatgacg
      121 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtga gctttgagac
      181 cacgtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      241 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      301 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      361 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
      421 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      481 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
      541 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
      601 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
      661 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
      721 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
      781 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
      841 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
      901 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
      961 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1021 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1081 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1141 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1201 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1261 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1321 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1381 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1441 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1501 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1561 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1621 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1681 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1741 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1801 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1861 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1921 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1981 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2041 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2101 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2161 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2221 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2281 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     2341 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2401 tcactgt
//
