LOCUS       sequence-495521-        2851 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2851)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50050
FEATURES             Location/Qualifiers
     source          1..2851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..101
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             42..131
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             156..179
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(651..669)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        737..841
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1063..1854
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2025..2613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2514..2533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2767..2784
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc ggaacaaaag ttgatctctg aagaggacct tgagcagaaa ttgatctctg
       61 aggaagatct tgagcagaag cttatctcag aggaggattt ggagcaaaaa cttatttctg
      121 aagaggacct tggtggttct ggaggtggtg ctagccttga ggttttgttc cagggtcctg
      181 gaagcggagg tggatcaggt ggatcaatgg agagtaaacg taataagcca ggtaaggcaa
      241 caggtaaagg taaaccagtt ggtgataaat ggcttgatga tgcaggtaaa gattcaggag
      301 caccaattcc agatcgtatt gctgataagt tgcgtgataa agaatttaaa agcttcgacg
      361 attttcgtaa ggctgtttgg gaagaggtgt caaaggatcc tgagcttagt aaaaatttga
      421 acccaagcaa taagtctagt gtttcaaaag gttattctcc atttactcca aagaatcaac
      481 aggtcggagg tagaaaagtc tatgaacttc atgctgacaa gccaattagt caaggtggtg
      541 aggtttatga catggataat atcagagtga ctacacctaa gagacatatc gatattcacc
      601 gtggtaagta atgagctttg agaccacgtg gctcttcagt ggacgaaagg gcctcgtgat
      661 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      781 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      841 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
      901 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
      961 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1021 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1081 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1141 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     1201 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1261 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     1321 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1381 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     1441 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1501 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1561 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1621 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     1681 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     1741 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1801 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     1861 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1921 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1981 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2041 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2101 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2161 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2221 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2281 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2341 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2401 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2461 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2521 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2581 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2641 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2701 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2761 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2821 cccgcgcgtt ggccgattca ttaatcactg t
//
