LOCUS       sequence-495523-        3426 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3426)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50058
FEATURES             Location/Qualifiers
     source          1..3426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1145..1186
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1226..1244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1312..1416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1638..2429
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2600..3188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3089..3108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3342..3359
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggaggcggt tcaggtggcg gatcgggcgg tggatcaatg gctcgggatc
       61 caccggtcgc caccatgaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta
      121 atggcgagtt ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta
      181 tcaacaagca cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa
      241 agggctactc tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc
      301 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
      361 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
      421 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
      481 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
      541 tggcagaagc gctgcgaaag ctgggagcag acaaggtact tcatgcttca acgtgtaact
      601 taagagatac tgtgtgaaat tttatatttc catacatttg cttgaccttt gctttttgtc
      661 aatttttttc cccttacagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag
      721 aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt
      781 cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg
      841 ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctacgct
      901 gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct
      961 gccacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga
     1021 caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca
     1081 ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc
     1141 tagcggcaag cccatcccca accccctgct gggcctggac agcacctaag ctttgagacc
     1201 acgtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     1261 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1381 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     1441 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     1501 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     1561 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     1621 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
     1681 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     1741 gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg gttacggtga
     1801 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     1861 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     1921 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     1981 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     2041 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     2101 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     2161 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     2221 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     2281 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     2341 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat tacgtaaaag
     2401 gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt
     2461 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2521 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2581 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2641 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2701 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2761 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2821 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2881 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2941 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3001 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3061 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3121 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3181 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3301 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3361 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3421 cactgt
//
