LOCUS       sequence-495524-        2362 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2362)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50059
FEATURES             Location/Qualifiers
     source          1..2362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             45..65
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             102..122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(162..180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        248..352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             574..1365
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1536..2124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2025..2044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2278..2295
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggtaaaaga accgccgacg gcagcgaatt cgagcctaag aagaagagga
       61 aggtttcagg aaagagaact gctgatggaa gtgagttcga gccaaagaag aagagaaagg
      121 tttgagcttt gagaccacgt ggctcttcag tggacgaaag ggcctcgtga tacgcctatt
      181 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgcgctc
      361 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
      421 catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc atccaagcag
      481 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      541 cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat cgccgaagta
      601 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
      661 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag cgatattgat
      721 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
      781 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
      841 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
      901 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
      961 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     1021 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     1081 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     1141 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     1201 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     1261 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     1321 gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc agaccaagtt
     1381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2341 tggccgattc attaatcact gt
//
