LOCUS       sequence-495525-        3148 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50060
FEATURES             Location/Qualifiers
     source          1..3148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..53
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             57..98
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             117..887
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(948..966)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1034..1138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1360..2151
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2322..2910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2811..2830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3064..3081
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gggtaagcca atccctaatc ctctcttggg tttggattct actggtggaa
       61 aacctattcc aaacccattg ctcggactcg actcaacccg cggatccgaa ttcatgatcc
      121 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
      181 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
      241 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
      301 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
      361 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
      421 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
      481 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
      541 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
      601 tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg atccgagagc
      661 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg
      721 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
      781 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
      841 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagaag cttgcggccg
      901 cactcgagtg agctttgaga ccacgtggct cttcagtgga cgaaagggcc tcgtgatacg
      961 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1141 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     1201 ggttttcatg gcttgttatg actgtttttt tttggggtac agtctatgcc tcgggcatcc
     1261 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1321 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc
     1381 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     1441 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagcgat
     1501 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     1561 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     1621 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     1681 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     1741 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     1801 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     1861 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     1921 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     1981 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     2041 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     2101 gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata actgtcagac
     2161 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2221 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2461 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3061 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3121 gcgcgttggc cgattcatta atcactgt
//
