LOCUS       sequence-495526-        3070 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50061
FEATURES             Location/Qualifiers
     source          1..3070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             15..104
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             45..134
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(870..888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        956..1060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1282..2073
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2244..2832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2733..2752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2986..3003
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggaacag aagctcatca gcgaagagga tctcgagcag aagctgatct
       61 ctgaggaaga tcttgagcaa aagctcattt ccgaagagga cttggagcaa aagttgatta
      121 gtgaagagga cctcatggtg agcaagggcg aagctgtgat caaagaattc atgaggttca
      181 aggtgcacat ggaaggcagc atgaacggac atgagttcga gatcgaaggt gaaggtgagg
      241 gtagacctta cgagggaact cagactgcta agctcaaggt gacaaaaggt ggaccgcttc
      301 cgttctcttg ggatatcctt tcaccgcagt tcatgtacgg cagcagggct ttcatcaagc
      361 accctgctga tatcccggac tactacaagc aatctttccc agagggattc aagtgggagc
      421 gtgtgatgaa tttcgaggat ggtggtgctg tgactgtgac ccaggatact tctcttgagg
      481 atgggaccct catctacaag gtgaagctca ggggaactaa cttccctcct gatggacctg
      541 tgatgcagaa aaagaccatg ggctgggaag cttctaccga gagactttat cctgaggacg
      601 gtgtgctcaa gggcgatatc aagatggctc ttaggcttaa ggacggtgga cgttacctcg
      661 ctgatttcaa gactacctac aaggccaaga aaccggtgca aatgcctggg gcttacaacg
      721 ttgaccgtaa gctcgatatc accagccaca acgaggatta cactgtggtt gagcagtacg
      781 agagatctga gggtcgtcat tctaccggtg gtatggacga gctttacaag tgagctttga
      841 gaccacgtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      901 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac gcaactggtc
     1081 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     1141 tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     1201 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     1261 cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
     1321 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     1381 tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt gctggttacg
     1441 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     1501 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     1561 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     1621 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     1681 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     1741 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     1801 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     1861 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     1921 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     1981 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccattacgta
     2041 aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta ctcatatata
     2101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3001 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3061 taatcactgt
//
