LOCUS       sequence-495528-        3151 bp    DNA     circular SYN 17-DEC-2025
DEFINITION  Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3151)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL50063
FEATURES             Location/Qualifiers
     source          1..3151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..719
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(157..175)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             720..809
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             822..848
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             852..878
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             885..911
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(951..969)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1037..1141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1363..2154
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2325..2913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2814..2833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3067..3084
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtctcattc gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
       61 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      121 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      181 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      241 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      301 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      361 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      421 acggccccgt aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc
      481 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      541 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      601 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      661 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      721 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat
      781 tgtatccata tgacgttcca gattacgcca ctagagctgc ttacccatac gatgttcctg
      841 actatgcggg ctatccctat gacgtcccgg actatgcagg attgtacccg tacgatgtgc
      901 ccgactacgc ttgagctttg agaccacgtg gctcttcagt ggacgaaagg gcctcgtgat
      961 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     1021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1141 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     1201 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     1261 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1321 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1381 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1441 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     1501 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1561 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     1621 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     1681 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     1741 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     1801 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     1861 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     1921 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     1981 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2041 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2101 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     2161 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2221 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2281 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2341 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2401 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2461 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2521 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2581 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2641 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2701 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2761 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2821 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2881 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2941 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3001 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3061 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3121 cccgcgcgtt ggccgattca ttaatcactg t
//
